Effects of Cardiolipin on Membrane Morphology: A Langmuir Monolayer Study

Phan, Minh Dinh; Shin, Kwanwoo

doi:10.1016/j.bpj.2015.03.026

Cited by 37 publications

(44 citation statements)

References 39 publications

(45 reference statements)

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“…32 CL can be considered as a dimer Figure 5 shows selected snapshots illustrating the organization of the mixed bilayers at the end of the simulations. Several features of the systems became apparent at the end of the simulation.…”

Section: Resultsmentioning

confidence: 99%

Effect of Phosphatidic Acid on Biomembrane: Experimental and Molecular Dynamics Simulations Study

et al. 2015

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We consider the impact of phosphatidic acid (namely, 1,2-dioleoyl-sn-glycero-3-phosphate, DOPA) on the properties of a zwitterionic (1,2-dipalmitoyl-sn-glycero-3-phosphocholine, DPPC) bilayer used as a model system for protein-free cell membranes. For this purpose, experimental measurements were performed using differential scanning calorimetry and the Langmuir monolayer technique at physiological pH. Moreover, atomistic-scale molecular dynamics (MD) simulations were performed to gain information on the mixed bilayer's molecular organization. The results of the monolayer studies clearly showed that the DPPC/DOPA mixtures are nonideal and the interactions between lipid species change from attractive, at low contents of DOPA, to repulsive, at higher contents of that component. In accordance with these results, the MD simulations demonstrated that both monoanionic and dianionic forms of DOPA have an ordering and condensing effect on the mixed bilayer at low concentrations. For the DOPA monoanions, this is the result of both (i) strong electrostatic interactions between the negatively charged oxygen of DOPA and the positively charged choline groups of DPPC and (ii) conformational changes of the lipid acyl chains, leading to their tight packing according to the so-called "umbrella model", in which large headgroups of DPPC shield the hydrophobic part of DOPA (the conical shape lipid) from contact with water. In the case of the DOPA dianions, cation-mediated clustering was observed. Our results provide a detailed molecular-level description of the lipid organization inside the mixed zwitterionic/PA membranes, which is fully supported by the experimental data.

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Section: Resultsmentioning

confidence: 99%

Effect of Phosphatidic Acid on Biomembrane: Experimental and Molecular Dynamics Simulations Study

et al. 2015

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“…The folds were observed in large 2D length-scales by mean of fluorescence microscopy Fig. 3A ; however, they were too local to be detected by X-ray reflectivity measurements 37 .…”

Section: Topography Of Collapsementioning

confidence: 89%

“…Consequently, polarization modulation-infrared reflection absorption spectroscopy PM-IRRAS 54 , X-ray diffuse scattering, X-ray diffraction 53 and X-ray reflectivity 37,52,53,55 studies have been used to examine collapse. For example, Ibn-Elhaj et al 52 performed X-ray reflectivity measurements of a three-block siloxane smectic liquid crystal on concentrated salt solutions Fig.…”

Section: E X P E R I M E N Ta L T E C H N I Q U E S To S T U Dy Collapsementioning

confidence: 99%

Collapsed States of Langmuir Monolayers

2016

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“…Indeed, several studies show CL content regulates microviscosity, packing, morphology, and phase behavior in isolated mitochondria and model membranes [25-34]. In contrast, no lab has completely discriminated the effects of CL concentration compared to CL acyl chain remodeling on membrane organization.…”

Section: 0 Introductionmentioning

confidence: 99%

Distinct membrane properties are differentially influenced by cardiolipin content and acyl chain composition in biomimetic membranes

Pennington¹,

Fix²,

Sullivan³

et al. 2017

Biochimica et Biophysica Acta (BBA) - Biomembranes

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Cardiolipin (CL) has a critical role in maintaining mitochondrial inner membrane structure. In several conditions such as heart failure and aging, there is loss of CL content and remodeling of CL acyl chains, which are hypothesized to impair mitochondrial inner membrane biophysical organization. Therefore, this study discriminated how CL content and acyl chain composition influenced select properties of simple and complex mitochondrial mimicking model membranes. We focused on monolayer excess area/molecule (a measure of lipid miscibility), bilayer phase transitions, and microdomain organization. In monolayer compression studies, loss of tetralinoleoyl [(18:2)4] CL content decreased the excess area/molecule. Replacement of (18:2)4CL acyl chains with tetraoleoyl [(18:1)4] CL or tetradocosahexaenoyl [(22:6)4] CL generally had little influence on monolayer excess area/molecule; in contrast, replacement of (18:2)4CL acyl chains with tetramyristoyl [(14:0)4] CL increased monolayer excess area/molecule. In bilayers, calorimetric studies showed that substitution of (18:2)4CL with (18:1)4CL or (22:6)4CL lowered the phase transition temperature of phosphatidylcholine vesicles whereas (14:0)4CL had no effect. Finally, quantitative imaging of giant unilamellar vesicles revealed differential effects of CL content and acyl chain composition on microdomain organization, visualized with the fluorescent probe Texas Red DHPE. Notably, microdomain areas were decreased by differing magnitudes upon lowering of (18:2)4CL content and substitution of (18:2)4CL with (14:0)4CL or (22:6)4CL. Conversely, exchanging (18:2)4CL with (18:1)4CL increased microdomain area. Altogether, these data demonstrate that CL content and fatty acyl composition differentially target membrane physical properties, which has implications for understanding how CL regulates mitochondrial activity and the design of CL-specific therapeutics.

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Effects of Cardiolipin on Membrane Morphology: A Langmuir Monolayer Study

Cited by 37 publications

References 39 publications

Effect of Phosphatidic Acid on Biomembrane: Experimental and Molecular Dynamics Simulations Study

Effect of Phosphatidic Acid on Biomembrane: Experimental and Molecular Dynamics Simulations Study

Collapsed States of Langmuir Monolayers

Distinct membrane properties are differentially influenced by cardiolipin content and acyl chain composition in biomimetic membranes

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