Thermal lens spectrometry in electromigration methods of analysis

Проскурнин, М. А.; Bendrysheva, S. N.; Smirnova, A. P.

doi:10.1134/s1061934816050105

Cited by 10 publications

(2 citation statements)

References 103 publications

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“…This may be linked with slow heat dissipation from the separation capillary and further experiments are currently underway to understand and fully optimize this approach. 47 As shown in the previous figures, photothermal absorption combined with BSI detection can improve both the selectivity and sensitivity of analyte detection in PCE-BSI separations. Photothermal signal enhancement may also provide new opportunities for studying binding interactions with CE.…”

Section: Analyticalmentioning

confidence: 73%

“…Surprisingly, preliminary studies using modulation frequencies ranging from 50 Hz to 1 kHz did not result in a significant improvement in signal-to-noise over direct excitation measurements. This may be linked with slow heat dissipation from the separation capillary and further experiments are currently underway to understand and fully optimize this approach …”

Section: Resultsmentioning

confidence: 99%

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Photothermal Backscatter Interferometry for Enhanced Detection in Capillary Electrophoresis

Opallage,

De Silva,

Kariuki

et al. 2024

Anal. Chem.

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Refractive index (RI) detection using backscatter interferometry (BSI) enables universal detection in capillary electrophoresis (CE). BSI detection is a versatile on-capillary approach that is easily integrated with capillary or microfluidic channels, straightforward to miniaturize, and inexpensive. The focused BSI light source can also double as the excitation source for fluorescence, enabling simultaneous universal (BSI) and specific (fluorescence) signals from the same detection volume. To improve BSI detection and expand orthogonal content, we integrate photothermal absorption with BSI detection. Nonradiative relaxation of an excited analyte releases heat into the surroundings, which modifies both the local RI and conductivity (viscosity) of the analyte zone. We recently showed that the BSI signal is sensitive to both RI and conductivity, which makes photothermal absorption a promising route to signal enhancement. Here, we use coaxially delivered BSI and photothermal absorption beams to characterize BSI, photothermal BSI, and fluorescence detection using the separation of test samples. We show that photothermal absorption leads to 3 orders of magnitude improvement in BSI detection limits at the powers studied and provides new opportunities for studying binding interactions with CE.

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Section: Analyticalmentioning

confidence: 73%

Section: Resultsmentioning

confidence: 99%