Thermal effects of shoulder electrothermal arthroscopic capsulorrhaphy monitored by differential scanning calorimetry—A preliminary study

Bognár, G.; Szabó, I; Bálint, L.; Hepp, B.; Kereskai, László; Lőrinczy, D.

doi:10.1016/j.tca.2007.07.020

Cited by 6 publications

(3 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The SI increase in combination with the decrease indicated that this sub-population persisted in both L 1 and L 2 . This additional lower-temperature peak was reported to result from physical treatment of the collagenous samples [ 12 ],[ 36 , 37 ]. The formation of a damaged intermediate state prior to the degradation of molecules to random coils has been proposed leading to reduction of the molecular stability due to the lattice structural damage.…”

Section: Discussionmentioning

confidence: 96%

Laser-induced modification of the patellar ligament tissue: comparative study of structural and optical changes

et al. 2010

View full text Add to dashboard Cite

The effects of non-ablative infrared (IR) laser treatment of collagenous tissue have been commonly interpreted in terms of collagen denaturation spread over the laser-heated tissue area. In this work, the existing model is refined to account for the recently reported laser-treated tissue heterogeneity and complex collagen degradation pattern using comprehensive optical imaging and calorimetry toolkits. Patella ligament (PL) provided a simple model of type I collagen tissue containing its full structural content from triple-helix molecules to gross architecture. PL ex vivo was subjected to IR laser treatments (laser spot, 1.6 mm) of equal dose, where the tissue temperature reached the collagen denaturation temperature of 60 ± 2°C at the laser spot epicenterin the first regime, and was limited to 67 ± 2°C in the second regime. The collagen network was analyzed versus distance from the epicenter. Experimental characterization of the collagenous tissue at all structural levels included cross-polarization optical coherence tomography, nonlinear optical microscopy, light microscopy/histology, and differential scanning calorimetry. Regressive rearrangement of the PL collagen network was found to spread well outside the laser spot epicenter (>2 mm) and was accompanied by multilevel hierarchical reorganization of collagen. Four zones of distinct optical and morphological properties were identified, all elliptical in shape, and elongated in the direction perpendicular to the PL long axis. Although the collagen transformation into a random-coil molecular structure was occasionally observed, it was mechanical integrity of the supramolecular structures that was primarily compromised. We found that the structural rearrangement of the collagen network related primarily to the heat-induced thermo-mechanical effects rather than molecular unfolding. The current body of evidence supports the notion that the supramolecular collagen structure suffered degradation of various degrees, which gave rise to the observed zonal character of the laser-treated lesion.

show abstract

Section: Discussionmentioning

confidence: 96%

Laser-induced modification of the patellar ligament tissue: comparative study of structural and optical changes

et al. 2010

View full text Add to dashboard Cite

show abstract

“…This was a feasibility study undertaken to determine whether there were any structural changes in the cartilaginous tissue in the shoulder treated by ETC [34]. DSC results showed a significant difference in the melting transitions, which are reported as 1.5 °C for the untreated tissue compared to 8 °C for the treated tissue, indicating much less cooperativity and lower thermal stability.…”

Section: Disease Statesmentioning

confidence: 99%

Thermoanalytical methods applied to medicine

Glass

Brown

2009

Pure and Applied Chemistry

View full text Add to dashboard Cite

Thermoanalytical methods have found increasing application in medicine due to the improved sensitivity and usability of the available instrumentation. Studies have identified important findings applied to medicine, including information on the thermal properties of the skin and the effect of insertion into body cavities of implants and prosthetics. These studies have explored the thermal stability of various materials to provide insight into drug penetration in order to design drug delivery systems, which are not only safe but capable of delivering improved and predictable therapeutic outcomes for patients. Differential scanning calorimetry (DSC) has also been applied to the study of disease states such as diabetes, where changes in the collagen structure of the skin, which may lead to long-term complications in these patients, can be detected. Although these results may at this stage not have significant clinical implications, they do provide medical researchers with a starting point for future investigations. The application of these techniques has been further extended to examinations of body systems and other disease states. The key for the future will be the ability of these techniques not only to provide information on alterations in these biological systems, but also to determine whether these alterations are clinically relevant.

show abstract

“…From the point of view of therapy, a procedure faster than microbiological tests would be useful. Differential scanning calorimetry (DSC) is an increasingly widely used test method for various pathologic, traumatologic, and orthopedic cases [2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18]. Typical symptoms of septic arthritis (high pain, swelling, redness, fever, warmer tact) do not always occur.…”

Section: Introductionmentioning

confidence: 99%

Thermal analysis of synovial fluids in different stages of osteoarthritis and after bacterial infections

Dandé

Kocsis

Lőrinczy

2020

J Therm Anal Calorim

View full text Add to dashboard Cite

The analysis of synovial fluid is an important method in diagnosing and handling septic arthritis. To achieve a quick diagnosis could be a great advantage in the therapy. The differential scanning calorimetry (DSC) proved to be a useful technique in the differential diagnosis of tumors using blood plasma or sera. The aim of this paper is to show up some characteristic thermal parameters in the diagnosis of different grades of arthritis, which are in good agreement with the severity of disease checked by conventional X-ray supported grading. To follow the effect of different bacterial strains, the synovial fluids were inoculated by three types of bacterial strains (with 103–105 CFU mL−1 concentrations) at 37 °C and stored trough 24 h. After that, they were denatured in 20–90 °C temperature range with 0.3 K min−1 scanning rate. The change in the maximum denaturation temperature (Tm) and calorimetric enthalpy (∆H) monitored the severity of sepsis and depended on the type of bacteria. The proliferation characteristics of bacteria should be strain specific. The synovial fluid samples inoculated with the most frequently occurring bacteria were monitored in isotherm mode (isoperibol calorimeter) at 37 °C up to the end of the proliferation. The isoperibolic scans clearly demonstrated specific, concentration-dependent representative curves in case of each bacterium (duration of proliferation, maximum of proliferation rates, etc.). Therefore, thermal analysis of human synovial fluid samples by DSC or isoperibolic calorimetry could be a useful tool in the staging of osteoarthritis and the diagnostics of septic arthritis.

show abstract

Thermal effects of shoulder electrothermal arthroscopic capsulorrhaphy monitored by differential scanning calorimetry—A preliminary study

Cited by 6 publications

References 29 publications

Laser-induced modification of the patellar ligament tissue: comparative study of structural and optical changes

Laser-induced modification of the patellar ligament tissue: comparative study of structural and optical changes

Thermoanalytical methods applied to medicine

Thermal analysis of synovial fluids in different stages of osteoarthritis and after bacterial infections

Contact Info

Product

Resources

About