Thermal analysis of synovial fluids in different stages of osteoarthritis and after bacterial infections

Abstract

The analysis of synovial fluid is an important method in diagnosing and handling septic arthritis. To achieve a quick diagnosis could be a great advantage in the therapy. The differential scanning calorimetry (DSC) proved to be a useful technique in the differential diagnosis of tumors using blood plasma or sera. The aim of this paper is to show up some characteristic thermal parameters in the diagnosis of different grades of arthritis, which are in good agreement with the severity of disease checked by conventional X-ray supported grading. To follow the effect of different bacterial strains, the synovial fluids were inoculated by three types of bacterial strains (with 103–105 CFU mL−1 concentrations) at 37 °C and stored trough 24 h. After that, they were denatured in 20–90 °C temperature range with 0.3 K min−1 scanning rate. The change in the maximum denaturation temperature (Tm) and calorimetric enthalpy (∆H) monitored the severity of sepsis and depended on the type of bacteria. The proliferation characteristics of bacteria should be strain specific. The synovial fluid samples inoculated with the most frequently occurring bacteria were monitored in isotherm mode (isoperibol calorimeter) at 37 °C up to the end of the proliferation. The isoperibolic scans clearly demonstrated specific, concentration-dependent representative curves in case of each bacterium (duration of proliferation, maximum of proliferation rates, etc.). Therefore, thermal analysis of human synovial fluid samples by DSC or isoperibolic calorimetry could be a useful tool in the staging of osteoarthritis and the diagnostics of septic arthritis.

Calorimetric Markers for Detection and Monitoring of Multiple Myeloma

“…This biophysical technique is classically applied to study and thermodynamically describe the stability and conformational transitions of biomolecules in solution. However, DSC is also suitable to analyze complex biofluids such as blood plasma/serum [ 3 , 4 , 5 ], cerebrospinal fluid [ 6 , 7 ], and synovial fluid [ 8 ], the calorimetric profiles (thermograms or scans) of which are a complex mixture of the thermal transitions of the individual biofluid components. DSC profiles depend not only on the plasma/serum proteins content and conformation but also on factors that moderate their stability such as ligand binding, macromolecular complex formation, mutations, chemical changes, protein oligomerization, aggregation and misfolding.…”

Increased Synovial CD14 mRNA Expression and Proportion of CD14high Subsets in Early-Stage Hip Osteoarthritis: Propensity Matched Score Analysis

“…It was difficult to distinguish between CD14 high and CD14 low cells using immunohistochemical procedures; the localization of these cells in the synovium remains unclear. Recently, methods such as the thermal analysis of synovial fluid, which are lower in cost, were developed to diagnose EOA and LOA [ 33 ]. Further investigation regarding the actual cost and accuracy will be necessary.…”

Thermal analysis of the biceps tendon samples obtained from patients underwent reversed shoulder arthroplasty for comminuted proximal humerus fractures

“…Detachment and tenodesis of this part of LHBT is a standard surgical procedure during shoulder replacements. Then the samples were preserved for further measurements as detailed elsewhere [11][12][13]. The control sample was taken from a macroscopically healthy segment of a biceps tendon, confirmed by histological examination.…”