Thermal Denaturation of Tobacco Mosaic Virus

A Bioengineered Positive Control for Rapid Detection of the Ebola Virus by Reverse Transcription Loop-Mediated Isothermal Amplification (RT-LAMP)

“…We demonstrated the stability of the EBOV-LAMP-TMVshort probe and its application as a positive control for diagnostics using RT-LAMP. Data indicate that the synthesized particle is stable for over a month when stored in aqueous buffer and at room temperature and, based on the established thermostability of TMV, , the engineered control samples are expected withstand the temperature conditions in West Africa. Nevertheless, if deemed necessary, the TMV-based EBOV control could be protected by storing and shipping the samples in a simple polystyrene cooler.…”

“…In fact, it has been shown that TMV nucleoprotein complexes are extremely stable and preparations of TMV have been reported to be stable for at least 50 years in nonsterile extracts stored at room temperature. 30 Furthermore, studies on the thermostability of TMV indicate that purified virus is stable at temperatures of up to 75 °C, 31 well above temperatures that the particle would be exposed to in West Africa. While we have not performed detailed thermostability studies on the proposed EBOV-LAMP-TMVshort control, based on the inherent stability of the TMV nucleoprotein complex, we hypothesize that also the shorter EBOV-LAMP-TMVshort assembly will have matched or comparable thermostability profiles.…”

Polymerization-Depolymerization of Tobacco Mosaic Virus Protein. VIII. Light-Scattering Studies*

“…Thus, it seems reasonable to assume that eq 1 is valid for the present study. Ansevin and Lauffer (1963) have demonstrated that the polymerization of tobacco mosaic virus protein is controlled by a second-order process, and point out that other data (Lauffer and Price, 1940;Lauffer and Dow, 1941;Lauffer, 1943) indicate that depolymerization may reasonably be considered a first-order process. They have suggested, therefore, that the equilibrium constant (K) for the polymerization of TMV-P can be expressed as the ratio of a secondorder rate constant (ki) for polymerization to a firstorder rate constant (k-i) for dissociation.…”

Kinetics of Thrombin Inactivation as Influenced by Physical Conditions, Trypsin, and Serum

“…), the value of it increases mark-edly to over 40,000 calories per mol (at constant pH). Such high values (under similar conditions) are characteristic of the thermal denaturation of proteins (3) and the heat inactivation of enzymes (38) and viruses (18). There is, therefore, a definite suggestion that, at about 60°C., thrombin is directly influenced by heating.…”

“…In enzyme systems, which show analogous behavior, such variability is often associated with the impurities present in the preparations, although many of the essential data are the same for crude preparations as for highly purified enzymes (37). In analogous systems, we also have the suggestion of specific intramolecular groups which act as negative catalysts (inhibitors), their effectiveness being lessened by dilution (18). Such an explanation may account for the greater stability of highly concentrated thrombin solutions, and for the marked deviations from the inverse law which they exhibit.…”