A Bioengineered Positive Control for Rapid Detection of the Ebola Virus by Reverse Transcription Loop-Mediated Isothermal Amplification (RT-LAMP)

Lam, Patricia; Keri, Ruth A.; Steinmetz, Nicole F.

doi:10.1021/acsbiomaterials.6b00769

Cited by 10 publications

(9 citation statements)

References 28 publications

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“…106,107 In addition to the rod structure, TMV can also take other forms such as stars and stripes, tripods, and boomerang structures. 108 The self-assembly of the TMV platform technology is well understood 109 and has led to wide-ranging applications in nanotechnology, including MRI imaging, 110 vaccine 111,112 and drug delivery, 78,113 Ebola diagnostics, 114,115 tissue engineering, 116,117 trinitrotoluene (TNT) sensing, 118 batteries and data storage, 119 and light harvesting. 120 Further applications are reviewed by Koch et al 121 Experiments to assess the potential for gene transfer using the TMV platform technology date back 30 years.…”

Section: The Cowpea Chlorotic Mottle Virus Platformmentioning

confidence: 99%

Plant viral and bacteriophage delivery of nucleic acid therapeutics

Lam

Steinmetz²

2017

WIREs Nanomed Nanobiotechnol

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Nucleic acid therapeutics have emerged as a powerful method for treatment of many diseases. However, the challenge lies in safe and efficient delivery of nucleic acids to their target site, as they need to cross various extracellular and intracellular barriers. Mammalian viruses have initially been favored for delivery of nucleic acid therapeutics, but safety concerns regarding their immunogenicity and potential of integration have fueled the search for alternative delivery strategies. For example, chemistry and bioengineering have led to advances in the use of nonviral vectors composed of lipids and other polymers; nevertheless, the synthetic systems often do not match the efficiency achieved using the biological systems. More recently, researchers have turned toward the development of plant viruses and bacteriophages and virus-like particles as an alternative or complementary approach. These systems unite the properties of both the viral and nonviral systems and as such are a new exciting avenue toward nucleic acid delivery. This review highlights the benefits of plant viral and bacteriophage delivery of nucleic acids and provides a summary of the current progress in research in this field. © 2017 Wiley Periodicals, Inc. How to cite this article:WIREs Nanomed Nanobiotechnol 2018, 10:e1487. doi: 10.1002/wnan.1487 NUCLEIC ACID THERAPEUTICS: OPPORTUNITIES AND CHALLENGESN ucleic acid-based therapeutics are comprised of wide-ranging forms of deoxyribonucleic acid (DNA) or ribonucleic acid (RNA). Therapeutic use of nucleic acids has had a long history, beginning with gene delivery, and its initial conceptualization over 40 years ago. 1 Since then, there has been an extensive body of work dedicated to gene therapy for treatment of diseases, such as Parkinson's, 2 cystic fibrosis, 3 hemophilia, 4 and cancer. 5 The discovery of small noncoding RNAs that regulate gene expression, 6 has led to the use of microRNAs (miRNAs) and small interfering RNAs (siRNAs) as possible therapeutics. 7 Nucleic acid-based therapies are on the horizon to become a clinical reality: the first approved RNA-based therapy in the market, Macugen, targets vascular endothelial growth factor (VEGF) in the eye to treat neovascular age-related macular degeneration. 8 UniQure's Glybera was approved for clinical use in Europe in 2012. 9 The adeno-associated virus (AAV)-based therapy is used to treat patients with lipoprotein lipase deficiency, an orphan metabolic disorder associated with increased levels of fat in the blood. Other nucleic acid-based therapies are poised to push toward commercialization in the coming year. 10,11 The road to bring gene of 18therapy to the market has not been an easy one, with tragedy striking in 1999 when a patient died during a clinical trial from adverse immune response instigated by the adenoviral vector meant to correct for ornithine transcarbamylase metabolic deficiency. 12 A few years later, despite great success for many children, retrovirus-based gene treatment for X-linked severe combined immunodeficiency resul...

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Section: The Cowpea Chlorotic Mottle Virus Platformmentioning

confidence: 99%

Plant viral and bacteriophage delivery of nucleic acid therapeutics

Lam

Steinmetz²

2017

WIREs Nanomed Nanobiotechnol

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“…[14,22,24,25] As such efforts have been made to develop ways to lengthen the RNA or cDNA prior to the PCR step adding another layer of complexity and cost to the assay. [12,[25][26][27] To simplify the instrumentation requirements, much research has focused on developing isothermal amplification techniques like RT-loop mediated isothermal amplification (LAMP) [25,28] and rolling circle amplification (RCA) [29] for POC diagnostics.…”

Section: Introductionmentioning

confidence: 99%

“…One challenge of isothermal methods like RT-LAMP and RCA commonly used in POC applications, is that they often require more than one enzyme and higher temperatures, the latter necessitating the use of heating instrumentation. [28][29][30] For example, RT-LAMP, like RT-PCR, utilizes reverse transcriptase in the first step to transcribe RNA into cDNA, [28] while for RCA an RNA-templated DNA ligation is performed with ligase to generate a circular template. [29,30] Next the cDNA is liberated from the RNA by digestion or nicking enzymes followed by amplification by polymerase.…”

Section: Introductionmentioning

confidence: 99%

“…[29,30] Next the cDNA is liberated from the RNA by digestion or nicking enzymes followed by amplification by polymerase. [11,28] These sequential steps to form the cDNA and then amplify it often lead to the use of multiple temperatures even when isothermal amplification methods are employed [31]. To overcome these problems and present a complementary approach to RT-polymerase based methods, we describe a simple, instrument-free method for detecting RNA that is completely performed at room temperature, uses only one enzyme (a ligase) and colorimetric detection.…”

Section: Introductionmentioning

confidence: 99%

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Reverse transcription lesion-induced DNA amplification: An instrument-free isothermal method to detect RNA

Alladin-Mustan

Liu

et al. 2021

Analytica Chimica Acta

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One challenge in point-of-care diagnostics is the lack of room-temperature methods for RNA detection based on enzymatic amplification and visualization steps. Here we perform a reverse transcription ligase chain reaction using our isothermal lesion induced DNA amplification (LIDA) technique that can be tuned to operate at any desired temperature. Using RNA-triggered LIDA, we can detect as little as ~100 attomoles target RNA and can distinguish RNA target from total cellular RNA. Finally, we demonstrate that the resulting DNA amplicons can be detected colorimetrically, also at room temperature, by rapid, targettriggered disassembly of DNA-modified gold nanoparticles. This integrated amplification/detection platform requires no heating or visualization instrumentation, which is an important step towards realizing instrument-free POC testing.

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“…The GR genes were used for rapid detection of the storage mite from sample materials including bacteria, host fungi, competitive fungi, and fungus gnats using loop-mediated isothermal amplification (LMAP). LAMP of DNA is an alternative to PCR-based methods in food safety testing 17 and a variety of other applications including detection of plant diseases and viruses in animals and plants 18 – 20 . Its advantages over PCR-based techniques include shorter reaction time, no need for specific equipment, high sensitivity and specificity, and comparably low susceptibility to inhibitors present in sample materials.…”

Section: Introductionmentioning

confidence: 99%

A Gustatory Receptor Used for Rapid Detection of Tyrophagus putrescentiae in Fungi Hosts

Wang

et al. 2018

Sci Rep

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The storage mite, Tyrophagus putrescentiae, found worldwide in many habitats, is an important pest of edible fungi in China. Storage mites are tiny and difficult to observe, especially when they occur in fungi composts. In this study, one gustatory receptor protein (TputGR1) was identified from the transcriptome of T. putrescentiae. Phylogenetic analysis of GRs families from 10 arthropod species revealed that TputGR1 had high homology with the SccaGR1 of Sarcoptes scabiei and TurtGR1-2 of Tetranychus urticae, but low homology with other insect species, Drosophila melanogaster, Anopheles gambiae, Bombyx mori, Aedes aegypti, Culex quinquefasciatus, and Pediculus humanus. We developed a detection system for the mite on fungi hosts using the GR protein and the loop-mediated isothermal amplification (LAMP). This procedure was rapid (60 min from sampling to result) and had high sensitivity (0.5 ng/mL). LAMP provided rapid and reliable detection of T. putrescentiae. It has good specificity for single samples and for large-scale surveys.

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A Bioengineered Positive Control for Rapid Detection of the Ebola Virus by Reverse Transcription Loop-Mediated Isothermal Amplification (RT-LAMP)

Cited by 10 publications

References 28 publications

Plant viral and bacteriophage delivery of nucleic acid therapeutics

Plant viral and bacteriophage delivery of nucleic acid therapeutics

Reverse transcription lesion-induced DNA amplification: An instrument-free isothermal method to detect RNA

A Gustatory Receptor Used for Rapid Detection of Tyrophagus putrescentiae in Fungi Hosts

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