A Gustatory Receptor Used for Rapid Detection of Tyrophagus putrescentiae in Fungi Hosts

Qu, Shaoxuan; Wang, Xiaofei; Li, Huiping; Luo, Xin; Ma, Lin

doi:10.1038/s41598-018-29729-4

Cited by 2 publications

(2 citation statements)

References 28 publications

(29 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…There are 68 GRs in D. melanogaster , 90 in Anopheles gambiae , 107 in Aedes aegypti and 126 in Culex quinquefasciatus 52 . Similarly, among different mites, 54 and 21 GRs are found in Metaseiulus occidentalis and Ixodes scapularis , respectively 53 . Phylogenetic analysis has shown that there are conserved orthologs of sugar, carbon dioxide and several orphan receptors between mosquitoes and D. melanogaster 54 .…”

Section: Discussionmentioning

confidence: 99%

“…52 Similarly, among different mites, 54 and 21 GRs are found in Metaseiulus occidentalis and Ixodes scapularis, respectively. 53 Phylogenetic analysis has shown that there are conserved orthologs of sugar, carbon dioxide and several orphan receptors between mosquitoes and D. melanogaster. 54 For example, AaGR34 and AgGR25 are orthologs of DGR43a.…”

Section: Mode Of Actions and Molecular Sensors Of Ir3535mentioning

confidence: 99%

See 1 more Smart Citation

Cellular and molecular basis of IR3535 perception in Drosophila

Shrestha

Pradhan

Nath

et al. 2021

Pest Management Science

View full text Add to dashboard Cite

BACKGROUND: IR3535 is among the most widely used synthetic insect repellents, particularly for the mitigation of mosquitoborne diseases such as malaria, yellow fever, dengue and Zika, as well as to control flies, ticks, fleas, lice and mites. These insects are well-known vectors of deadly diseases that affect humans, livestock and crops. Moreover, global warming could increase the populations of these vectors.RESULTS: Here, we performed IR3535 dose-response analyses on Drosophila melanogaster, a well-known insect model organism, using electrophysiology and binary food choice assays. Our findings indicated that bitter-sensing gustatory receptor neurons (GRNs) are indispensable to detect IR3535. Further, potential candidate gustatory receptors were screened, among which GR47a was identified as a key molecular sensor. IR3535 concentrations in the range 0.1-0.4% affected larval development and mortality. In addition, N,N-diethyl-m-toluamide (DEET, another commonly used insecticide) was found to exert synergistic effects when co-administered with IR3535. CONCLUSION: Our findings confirmed that IR3535 directly activates bitter-sensing GRNs, which are mediated by GR47a. This relatively safe and highly potent insecticide can be largely used in combination with DEET to increase its efficiency to protect livestock and crops. Collectively, our findings suggest that the molecular sensors elucidated herein could be used as targets for the development of alternative insecticides.

show abstract

Section: Discussionmentioning

confidence: 99%