Therapy with Dendritic Cells (DCs) control fetal abortion rate in the CBA/J × DBA/2J mouse model

npj Biofilms Microbiomes

Feng

et al. 2021

A dysregulation of cytokine networks has been suggested to be involved in the pathogenesis of unexplained pregnancy loss. Gut microbiota affects host immune response and induces an imbalance in cytokine levels. However, how gut microbial dysbiosis disturbs cellular immune function in miscarriage remains inconclusive. Here we report that IL-2, IL-17A, IL-17F, TNF-α, and IFN-γ are significantly increased in serum of miscarriage patients. Fecal microbiome analyses indicate that microbial diversity and the relative abundances of Prevotella_1, Prevotellaceae_UCG_003 and Selenomonas_1 are significantly reduced in the cases. Correlation analyses indicate that some microbe-associated metabolites are positively associated with changes in levels of Th1/Th17 cytokines in the miscarriage group. Moreover, we identify that imidazolepropionic acid and 1,4-methylimidazoleacetic acid are associated with subsequent recurrent miscarriage. Our study highlights the network among gut microbiota, fecal metabolites and Th1/Th17-mediated immune response in miscarriage patients and explores the potential predictive values of two fecal metabolites for recurrent miscarriages.

Section: Introductionmentioning

confidence: 99%

Interactions between gut microbiota and metabolites modulate cytokine network imbalances in women with unexplained miscarriage

npj Biofilms Microbiomes

Feng

et al. 2021

“…Thus, pregnancy has been labeled a 'Th2 phenomenon'. Blois et al showed that Th1 cytokines led to pregnancy loss in mice [6], and Chaouat et al demonstrated that this loss can be prevented by Th2 cytokines [7]. Inflammatory processes alter the balance of Th1 and Th2 cytokines and lead to a shift toward Th1 predominance.…”

Section: Introductionmentioning

confidence: 99%

Gut Dysbacteriosis Is Associated with an Imbalanced Cytokines Network in Women with Unexplained Miscarriage

et al. 2020

Preprint

Background: A dysregulation of cytokine networks has been suggested to be involved in the pathogenesis of unexplained pregnancy loss. Gut microbiota affects host immune response and induces an imbalance in cytokine levels. However, how gut microbial dysbiosis affects the disturbance of cellular immune function in spontaneous abortion (SA) patients remains inconclusive.Results: Serum proinflammatory cytokine levels were significantly increased in SA patients. Microbial diversity in patients with SA was reduced compared with that in the controls, as were the relative abundances of Prevotella_1, Prevotellaceae_UCG_003, and Selenomonas_1 were significantly reduced in the cases. Fecal metabolomics profiles were altered in SA patients, and the correlation analyses indicated that some microbe-associated metabolites, which were significantly enriched in bile secretion and histidine metabolism pathways, were positively associated with changes in levels of IL-17A, IL-17F, TNF-α, and IFN-γ in the SA group. Moreover, the microbial-associated metabolites, imidazolepropionic acid and 1, 4-Methylimidazoleacetic acid, are associated with recurrent pregnancy loss.Conclusions: Our study highlights the network among gut microbiome, fecal metabolites and Th1/Th17-mediated immune response in miscarriage patients and identifies potential novel biomarkers and an intervention target for recurrent miscarriage.

“…Thus, pregnancy has been labeled a 'Th2 phenomenon'. Blois et al showed that Th1 cytokines led to pregnancy loss in mice [6], and Chaouat et al demonstrated that this loss can be prevented by Th2 cytokines [7]. In ammatory processes alter the balance of Th1 and Th2 cytokines and lead to a shift toward Th1 predominance.…”

Section: Introductionmentioning

confidence: 99%

Gut Dysbacteriosis Is Associated with an Imbalanced Cytokines Network in Women with Unexplained Miscarriage

et al. 2020

Preprint

Background: A dysregulation of cytokine networks has been suggested to be involved in the pathogenesis of unexplained pregnancy loss. Gut microbiota affects host immune response and induces an imbalance in cytokine levels. However, how gut microbial dysbiosis affects the disturbance of cellular immune function in spontaneous abortion (SA) patients remains inconclusive.Results: Serum proinflammatory cytokine levels were significantly increased in SA patients. Microbial diversity in patients with SA was reduced compared with that in the controls, as were the relative abundances of Prevotella_1, Prevotellaceae_UCG_003, and Selenomonas_1 were significantly reduced in the cases. Fecal metabolites profiles were altered in SA patients, and the correlation analyses indicated that some microbe-associated metabolites, which were significantly enriched in bile secretion and histidine metabolism pathways, were positively associated with changes in levels of IL-17A, IL-17F, TNF-α, and IFN-γ in the SA group. Moreover, the microbial-associated metabolites, imidazolepropionic acid and 1, 4-Methylimidazoleacetic acid, are associated with recurrent pregnancy loss.Conclusions: Our study highlights the network among gut microbiota, fecal metabolites and Th1/Th17-mediated immune response in miscarriage patients and identifies potential novel biomarkers and an intervention target for recurrent miscarriage.