Therapeutic Protection Against H. pylori Infection in Mongolian Gerbils by Oral Immunization With a Tetravalent Epitope-Based Vaccine With Polysaccharide Adjuvant

Guo, Le; Hong, Dantong; Wang, Shue; Zhang, Fan; Tang, Feng; Wu, Tao; Chu, Yuankui; Liu, Hongpeng; Meng, He; Yang, Hua; Yin, Runsheng; Liu, Kunmei

doi:10.3389/fimmu.2019.01185

Cited by 24 publications

(19 citation statements)

References 42 publications

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“…In addition to these experiments in vitro, we also verified the effect of hydrogen peroxide on H. pylori in vivo. Mongolian gerbils are the most commonly used animal for establishing an H. pylori-infection model (37,38). The symptoms of H. pylori-infected gerbils, such as the loss of appetite and weight, were consistent with human patients.…”

Section: Discussionmentioning

confidence: 95%

Hydrogen Peroxide-Mediated Oxygen Enrichment Eradicates Helicobacter pylori In Vitro and In Vivo

Dounan

Zhang

Cao

et al. 2020

Antimicrob Agents Chemother

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Helicobacter pylori is an important risk factor for gastric ulcers. However, antibacterial therapies increase the resistance rate and decrease the eradication rate of H. pylori. Inspired by the microaerophilic characteristics of H. pylori, we aimed at effectively establishing an oxygen-enriched environment to eradicate and prevent the recurrence of H. pylori. The effect and the mechanism of an oxygen-enriched environment in eradicating H. pylori and preventing the recurrence were explored in vitro and in vivo. During oral administration and after drug withdrawal, H. pylori counts were evaluated by Giemsa staining in animal cohorts. An oxygen-enriched environment in which H. pylori could not survive was successfully established by adding hydrogen peroxide into several solutions and rabbit gastric juice. Hydrogen peroxide effectively killed H. pylori in Columbia blood agar and special peptone broth. Minimum inhibition concentrations and minimum bactericidal concentrations of hydrogen peroxide were both relatively stable after promotion of resistance for 30 generations, indicating that hydrogen peroxide did not easily promote resistance in H. pylori. In models of Mongolian gerbils and Kunming mice, hydrogen peroxide has been shown to significantly eradicate and effectively prevent the recurrence of H. pylori without toxicity and damage to the gastric mucosa. The mechanism of hydrogen peroxide causing H. pylori death was related to the disruption of bacterial cell membranes. The oxygen-enriched environment achieved by hydrogen peroxide eradicates and prevents the recurrence of H. pylori by damaging bacterial cell membranes. Hydrogen peroxide thus provides an attractive candidate for anti-H. pylori treatment.

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Section: Discussionmentioning

confidence: 95%

Hydrogen Peroxide-Mediated Oxygen Enrichment Eradicates Helicobacter pylori In Vitro and In Vivo

Dounan

Zhang

Cao

et al. 2020

Antimicrob Agents Chemother

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“…Oral therapeutic immunisation with FVpE plus PA significantly reduced the H pylori colonisation density in Mongolian gerbils. The protection of FVpE was related to the mixed Th cell responses and epitope‐specific antibodies against various H pylori antigens 27 …”

Section: Vaccinesmentioning

confidence: 99%

Review ‐ Helicobacter, inflammation, immunology and vaccines

Robinson

Lehours

2020

Helicobacter

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Understanding the mechanisms involved in induction and regulation of the immune and inflammatory response to Helicobacter pylori is extremely important in determining disease outcomes. H pylori expresses a plethora of factors that influence the host response. Vaccines against H pylori are desperately needed for the prevention of gastric carcinogenesis, especially with the increasing trends in antimicrobial resistance. This review summarizes some important findings, published between 1 April 2019 and 31 March 2020, in the areas of H pylori‐mediated inflammation, immunity and vaccines.

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“…pylori infection than rUreB 19 . Guo 20 recently discovered the FVpE vaccine, which is composed of cytotoxin‐associated antigen (CagA), urease, and vacuolating cytotoxin A (VacA) epitopes and a neutrophil‐activating protein (NAP) adjuvant. However, FVpE is better than the CTBUE vaccine in preventing and treating H .…”

Section: Introductionmentioning

confidence: 99%

Oral multivalent epitope vaccine, based on UreB, HpaA, CAT, and LTB, for prevention and treatment of Helicobacter pylori infection in C57BL / 6 mice

et al. 2021

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Background As the resistance of Helicobacter pylori to traditional triple therapy is gradually revealed, an increasing number of people are focusing on vaccine treatments for H. pylori infection. Epitope vaccines are a promising strategy for the treatment of H. pylori infection, and multivalent vaccines will be more effective than monovalent vaccines. Materials and methods In this study, we designed a multivalent vaccine named LHUC, which consists of the adjuvant LTB as well as three Th cell epitopes (HpaA154‐171, UreB237‐251, and UreB546‐561) and five B‐cell epitopes (UreB349‐363, UreB327‐334, CAT394‐405, CAT387‐397, and HpaA132‐141) from UreB, HpaA, and catalase. In BALB/c mice, the specificity and immunogenicity of the fusion peptide LHUC and the neutralization of H. pylori urease and catalase by the specific IgG elicited by LHUC were evaluated. The preventive and therapeutic effects of LHUC were evaluated in C57BL/6 mice infected with H. pylori. Results The results showed that compared with LTB and PBS, LHUC induced specific IgG and IgA antibody production in mice, and IgG antibodies significantly inhibited the H. pylori urease and catalase activities in vitro. Additionally, by detecting the levels of IFN‐γ, IL‐4, and IL‐17 in lymphocyte supernatants, we proved that LHUC could activate Th1, Th2, and Th17 mixed T‐cell immune responses in vivo. Finally, a C57BL/6 mouse model of gastric infection with H. pylori was established. The results showed that compared with the effects of LTB and PBS, the prevention and treatment effects of oral inoculation with LHUC significantly inhibited bacterial colonization. Conclusions In conclusion, LHUC, a multivalent vaccine based on multiple H. pylori antigens, is a promising and safe vaccine that can effectively reduce the colonization of H. pylori in the stomach.

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Therapeutic Protection Against H. pylori Infection in Mongolian Gerbils by Oral Immunization With a Tetravalent Epitope-Based Vaccine With Polysaccharide Adjuvant

Cited by 24 publications

References 42 publications

Hydrogen Peroxide-Mediated Oxygen Enrichment Eradicates Helicobacter pylori In Vitro and In Vivo

Hydrogen Peroxide-Mediated Oxygen Enrichment Eradicates Helicobacter pylori In Vitro and In Vivo

Review ‐ Helicobacter, inflammation, immunology and vaccines

Oral multivalent epitope vaccine, based on UreB, HpaA, CAT, and LTB, for prevention and treatment of Helicobacter pylori infection in C57BL / 6 mice

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