Therapeutic potential of triterpenoid saponin anemoside B4 from Pulsatilla chinensis

Li, Yan Hong; Zhang, Min; Han, Qiang; Deng, Li rong; Weinshilboum, Richard M.

doi:10.1016/j.phrs.2020.105079

Cited by 55 publications

(42 citation statements)

References 41 publications

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“…AB4 is one of the major active chemical ingredients in P. chinensis [ 11 ]. Previous studies supported its activity in anti-inflammatory and immune-modulatory properties [ 13 ].…”

Section: Discussionmentioning

confidence: 99%

“…Anemoside B4 (AB4) is a main effective triterpene saponin from the roots of Pulsatilla chinensis (Bunge) [ 10 ]. P. chinensis shows a considerable activity on anti-inflammatory and antioxidant [ 11 ]. Recent studies also identified the therapeutic potential of triterpenoid saponin AB4 in the treatment of hepatocellular carcinoma and chronic myeloid leukemia [ 12 , 13 ].…”

Section: Introductionmentioning

confidence: 99%

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Anemoside B4 sensitizes human colorectal cancer to fluorouracil-based chemotherapy through src-mediated cell apoptosis

He¹,

Tang²,

Huang³

et al. 2021

Aging

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Currently, 5-Fluorouracil (5-FU) based chemotherapy is the primary option for colorectal cancer after surgery, whereas chemotherapy resistance related mortality is observed in a large proportion of patients. Anemoside B4 (AB4) is a triterpene saponin, which exhibits a considerable activity in oncotherapy. In this study, we explored the efficacy of AB4 in FU-based chemotherapy in colorectal cancer cells and the underlying molecular mechanisms. Our results indicated a significant synergistic activity of AB4 in 5-FU treated colorectal cancer cells. Furthermore, AB4 treatment eliminated colorectal cancer stem cells by promoting apoptotic cell death in 5-FU resistant colorectal cancer cells. Mechanically, AB4 activated caspase-9 pathway in 5-FU resistant colorectal cancer cells. Elevated Src activity induced cell apoptosis and cancer stem cells elimination effects in AB4 treated colorectal cancer cells. In conclusion, AB4 showed promising sensitization effect in the FU-based chemotherapy of colorectal cancer. Our study may pave a way to ameliorate FU-based chemotherapeutic efficiency in colorectal cancer.

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“…AB4 is one of the major active chemical ingredients in P. chinensis [ 11 ]. Previous studies supported its activity in anti-inflammatory and immune-modulatory properties [ 13 ].…”

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Anemoside B4 sensitizes human colorectal cancer to fluorouracil-based chemotherapy through src-mediated cell apoptosis

He¹,

Tang²,

Huang³

et al. 2021

Aging

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“…Pulsatilla Decoction is famous formula prescription, and it is rich in triterpenoid saponins, such as Pulsatilla Saponin B4, Pulsatilla Saponin A3, and 23-hydroxybetulinic acid [12,13]. As the major active component of Pulsatilla chinensis, Pulsatilla saponin B4 has also received attention as a pure compound for its therapeutic potential.…”

Section: Discussionmentioning

confidence: 99%

Effect of a natural plant ingredient — Pulsatilla saponin B4 on clinical mastitis and serum inflammatory indices in dairy cows

Shen

Qian

You

et al. 2020

Preprint

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Background: Bovine mastitis is an inflammatory reaction of the breast caused by pathogenic bacteria infection or breast injury, which affect milk yield and quality and poses severe threat to the health of dairy cows. Pulsatilla chinensis has antibacterial, anti-inflammatory, and immune enhancing effects, etc. However, there was no research about the therapeutic effect of it towards the treatment of cow mastitis. In the study, twelve healthy cows were selected as the control group (group A), and 36 cows with mastitis were equally and randomly divided into group B, C and D. Group A didn’t accept treatment, while group B, C and D were treated with 15, 30 and 60 mL Pulsatilla saponin B4 (a purified extract form Pulsatilla chinensis) injection by brachiocephalicus intramuscular injection once a day for 4-6 day and the optimal dose was selected from them. Then, we investigated the effect of this extract on the clearance rates of pathogenic bacteria, the regulation of somatic cell count (SCC) and inflammatory factors the in appropriate dose group.Results: Pulsatilla saponin B4 was effective in all test groups and showed a dose-effect relationship. The extract had inhibitory effects on Streptococcus agalactiae, Streptococcus dysenteriae, Klebsiella species, Mycoplasma bovis, Escherichia coli and Staphylococcus aureus. Group C cured more cows than group B and D during the 6 days of treatment and was determined as the optimal dose group. The milk SCC, serum haptoglobin (HP), prostaglandin E2 (PGE2), interleukin-1 alpha (IL-1α), interleukin-1 beta (IL-1β), interleukin-2 (IL-2), interleukin-8 (IL-8), interleukin-10 (IL-10) and tumour necrosis factor alpha (TNF-α), interleukin-4 (IL-4) and leukotriene B4 (LTB4) in Group C were significantly higher than those of healthy cows (P < 0.05) on the first day but had no significant difference with group A after treatment. Conclusion: 30 mL Pulsatilla saponin B4 applied through intramuscular injection once a day for 4-6 days leaded to the significant amelioration of bacterial infection, SCC and levels of serum inflammatory factors in cows with clinical mastitis.

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“…There are numerous glycosyl derivatives of 23‐OH‐BA, such as the bidesmosidic saponins bourneiosides A and B, 75 and anemoside B4, a potent anti‐inflammatory and immune‐modulatory compound acting as an inhibitor of the NF‐κB signaling pathway 76,77 . This latter compound has been proposed for the treatment of ulcerative colitis and other inflammatory diseases 78–80 . We can also mention monodesmosidic saponins, such as (1) pulchinenoside A, isolated from Pulsatilla chinensis and endowed with a modest antiproliferative activity 18 and (2) wujiapiosides A and B, both isolated from Acanthopanax gracilistylus 81,82 .…”

Section: Acankoreosides: Glycoside Derivatives Of Ack and Analogsmentioning

confidence: 99%

Acankoreagenin and acankoreosides, a family of lupane triterpenoids with anti‐inflammatory properties: an overview

Bailly¹

2021

Annals of the New York Academy of Sciences

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Acankoreagenin (ACK, also known as acankoreanogenin and HLEDA) and impressic acid are two lupane‐type triterpenes that can be isolated from various Acanthopanax and Schefflera species. They efficiently block activation of the NF‐κB signaling pathway and the release of proinflammatory cytokines and/or the action of inflammation mediators (HMGB1, iNOS, and NO). These effects are the basis for the antiviral and anticancer activities reported with these pentacyclic compounds or their various glycoside derivatives. More than 15 acankoreosides (Ack‐A to ‐O, and ‐R) and a few other mono‐ and bidesmosidic saponins (acantrifoside A and acangraciliside S) derive from the ACK aglycone. Compounds like Ack‐A and ‐B are remarkable anti‐inflammatory agents, inhibiting cytokine release from activated macrophages. Despite their effectiveness, ACK and impressic acid are far much less known and studied than the structurally related compounds betulinic acid and 23‐hydroxybetulinic acid (anemosapogenin). The structural differences (notably the R/S stereoisomerism of the 3‐hydroxyl group) and functional similarities of these compounds are discussed. The complete series of acankoreosides is presented for the first time. These natural products deserve further attention as anti‐inflammatory agents, and ACK is recommended as a template for the design of new anticancer and antiviral drugs.

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Therapeutic potential of triterpenoid saponin anemoside B4 from Pulsatilla chinensis

Cited by 55 publications

References 41 publications

Anemoside B4 sensitizes human colorectal cancer to fluorouracil-based chemotherapy through src-mediated cell apoptosis

Anemoside B4 sensitizes human colorectal cancer to fluorouracil-based chemotherapy through src-mediated cell apoptosis

Effect of a natural plant ingredient — Pulsatilla saponin B4 on clinical mastitis and serum inflammatory indices in dairy cows

Acankoreagenin and acankoreosides, a family of lupane triterpenoids with anti‐inflammatory properties: an overview

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