Therapeutic Potential of Reactive Oxygen Species: State of the Art and Recent Advances

Graceffa, Valeria

doi:10.1177/2472630320977450

Cited by 11 publications

(7 citation statements)

References 219 publications

(274 reference statements)

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“…Based on these results, we could propose that artesunate induces cell senescence in SW480 and HCT116. ROS is considered to be a leading mediator of cell senescence [ 37 ]. Through NAC, a ROS scavenger, we revealed that artesunate caused ROS-dependent cell senescence ( Figure 3 B and Figure S1D ).…”

Section: Discussionmentioning

confidence: 99%

Artesunate Inhibits the Cell Growth in Colorectal Cancer by Promoting ROS-Dependent Cell Senescence and Autophagy

Zhong-jing

Gan

Zhuang

et al. 2022

Cells

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Although artesunate has been reported to be a promising candidate for colorectal cancer (CRC) treatment, the underlying mechanisms and molecular targets of artesunate are yet to be explored. Here, we report that artesunate acts as a senescence and autophagy inducer to exert its inhibitory effect on CRC in a reactive oxygen species (ROS)-dependent manner. In SW480 and HCT116 cells, artesunate treatment led to mitochondrial dysfunction, drastically promoted mitochondrial ROS generation, and consequently inhibited cell proliferation by causing cell cycle arrest at G0/G1 phase as well as subsequent p16- and p21-mediated cell senescence. Senescent cells underwent endoplasmic reticulum stress (ERS), and the unfolded protein response (UPR) was activated via IRE1α signaling, with upregulated BIP, IRE1α, phosphorylated IRE1α (p-IRE1α), CHOP, and DR5. Further experiments revealed that autophagy was induced by artesunate treatment due to oxidative stress and ER stress. In contrast, N-Acetylcysteine (NAC, an ROS scavenger) and 3-Methyladenine (3-MA, an autophagy inhibitor) restored cell viability and attenuated autophagy in artesunate-treated cells. Furthermore, cellular free Ca2+ levels were increased and could be repressed by NAC, 3-MA, and GSK2350168 (an IRE1α inhibitor). In vivo, artesunate administration reduced the growth of CT26 cell-derived tumors in BALB/c mice. Ki67 and cyclin D1 expression was downregulated in tumor tissue, while p16, p21, p-IRE1α, and LC3B expression was upregulated. Taken together, artesunate induces senescence and autophagy to inhibit cell proliferation in colorectal cancer by promoting excessive ROS generation.

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Section: Discussionmentioning

confidence: 99%

Artesunate Inhibits the Cell Growth in Colorectal Cancer by Promoting ROS-Dependent Cell Senescence and Autophagy

Zhong-jing

Gan

Zhuang

et al. 2022

Cells

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“…This may be the result of the nanostructured and hydroxylated surface of the H-TNTs/f-Ti composite, which is important for accelerating initial protein adsorption and influencing the cell proliferation [26]. In addition, low oxidative stress was furthermore proven to activate pathways leading to proliferation, migration and collagen synthesis of cells [49]. Following visible light activation, H-TNTs/f-Ti composite showed strong antimicrobial activity against bacteria and were also biocompatible with preosteoblast cells.…”

Section: Discussionmentioning

confidence: 99%

Visible light-induced antibacterial and osteogenic cell proliferation properties of hydrogenated TiO₂ nanotubes/Ti foil composite

Zhao

Wang

et al. 2021

Nanotechnology

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We successfully fabricated the hydrogenated TiO2 nanotubes/Ti foil (H-TNTs/f-Ti) composite via one-step anodization and two-step annealing. H-TNTs/f-Ti composite had a higher visible light-induced photoelectric response and more hydroxyl functional groups compared with Ti foil and unmodified TiO2 nanotubes/Ti foil composite, which contributed to limiting the proliferation of Streptococcus mutans and Porphyromonas gingivalis, promoting the proliferation of MC3T3-E1 cell on the hydroxylated surface, and improving the biocompatibility with osteogenic cells. Our study provides a simple and effective method for significantly improving dental implant efficacy.

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“…They also led to higher cell spreading and transfection efficiency, measured 2, 4 and 6 days after seeding [161]. The reason NIH3T3 murine fibroblasts PEI polyplexes In RGD-functionalised PAA brushes, luciferase expression (luminescence/mg protein) was 2.5 × 10 8 . In non-functionalised PAA brushes, it was ∼ 7 × 10 6 .…”

Section: Peptide Specificitymentioning

confidence: 99%

“…DNA polyplexes or lipoplexes can be embedded, immobilised or coated on biomaterials. Not only do the biomaterials increase localisation at the desired site, offer a support for cells to grow and a mechanical support [2,8], but they also directly modulate transfection efficiency. As a matter of fact, cell embedding [9], cell adhesion [10,11] and migration [12,13] influence cytoskeletal arrangement and focal adhesions, ultimately affecting DNA internalisation and nuclear transport.…”

Section: Introductionmentioning

confidence: 99%

Physical and mechanical cues affecting biomaterial-mediated plasmid DNA delivery: insights into non-viral delivery systems

Graceffa

2021

Journal of Genetic Engineering and Biotechnology

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Background Whilst traditional strategies to increase transfection efficiency of non-viral systems aimed at modifying the vector or the polyplexes/lipoplexes, biomaterial-mediated gene delivery has recently sparked increased interest. This review aims at discussing biomaterial properties and unravelling underlying mechanisms of action, for biomaterial-mediated gene delivery. DNA internalisation and cytoplasmic transport are initially discussed. DNA immobilisation, encapsulation and surface-mediated gene delivery (SMD), the role of extracellular matrix (ECM) and topographical cues, biomaterial stiffness and mechanical stimulation are finally outlined. Main text Endocytic pathways and mechanisms to escape the lysosomal network are highly variable. They depend on cell and DNA complex types but can be diverted using appropriate biomaterials. 3D scaffolds are generally fabricated via DNA immobilisation or encapsulation. Degradation rate and interaction with the vector affect temporal patterns of DNA release and transgene expression. In SMD, DNA is instead coated on 2D surfaces. SMD allows the incorporation of topographical cues, which, by inducing cytoskeletal re-arrangements, modulate DNA endocytosis. Incorporation of ECM mimetics allows cell type-specific transfection, whereas in spite of discordances in terms of optimal loading regimens, it is recognised that mechanical loading facilitates gene transfection. Finally, stiffer 2D substrates enhance DNA internalisation, whereas in 3D scaffolds, the role of stiffness is still dubious. Conclusion Although it is recognised that biomaterials allow the creation of tailored non-viral gene delivery systems, there still are many outstanding questions. A better characterisation of endocytic pathways would allow the diversion of cell adhesion processes and cytoskeletal dynamics, in order to increase cellular transfection. Further research on optimal biomaterial mechanical properties, cell ligand density and loading regimens is limited by the fact that such parameters influence a plethora of other different processes (e.g. cellular adhesion, spreading, migration, infiltration, and proliferation, DNA diffusion and release) which may in turn modulate gene delivery. Only a better understanding of these processes may allow the creation of novel robust engineered systems, potentially opening up a whole new area of biomaterial-guided gene delivery for non-viral systems.

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Therapeutic Potential of Reactive Oxygen Species: State of the Art and Recent Advances

Cited by 11 publications

References 219 publications

Artesunate Inhibits the Cell Growth in Colorectal Cancer by Promoting ROS-Dependent Cell Senescence and Autophagy

Artesunate Inhibits the Cell Growth in Colorectal Cancer by Promoting ROS-Dependent Cell Senescence and Autophagy

Visible light-induced antibacterial and osteogenic cell proliferation properties of hydrogenated TiO₂ nanotubes/Ti foil composite

Physical and mechanical cues affecting biomaterial-mediated plasmid DNA delivery: insights into non-viral delivery systems

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Therapeutic Potential of Reactive Oxygen Species: State of the Art and Recent Advances

Cited by 11 publications

References 219 publications

Artesunate Inhibits the Cell Growth in Colorectal Cancer by Promoting ROS-Dependent Cell Senescence and Autophagy

Artesunate Inhibits the Cell Growth in Colorectal Cancer by Promoting ROS-Dependent Cell Senescence and Autophagy

Visible light-induced antibacterial and osteogenic cell proliferation properties of hydrogenated TiO2 nanotubes/Ti foil composite

Physical and mechanical cues affecting biomaterial-mediated plasmid DNA delivery: insights into non-viral delivery systems

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Product

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Visible light-induced antibacterial and osteogenic cell proliferation properties of hydrogenated TiO₂ nanotubes/Ti foil composite