Therapeutic Potential of low-cost Nanocarriers Produced By Green Synthesis: Macrophage Uptake of Superparamagnetic Iron Oxide Nanoparticles

Verçoza, Brunno Renato Farias; Bernardo, Robson Roney; Pentón-Madrigal, A.; Sinnecker, J.P.; Rodrigues, Juliany Cola Fernandes; Oliveira, L. A. S. de

doi:10.2217/nnm-2018-0500

Cited by 17 publications

(27 citation statements)

References 64 publications

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“…The characteristic blue color developing from the reaction of potassium ferrocyanide with NP ferric ions highlighted a comparable macrophage dose-dependent uptake of nanoconjugated antibiotics or of their nanocarriers in 24h. That macrophages engulfed IONPs, NP-TEICO, and NP-VANCO in a comparable mode confirmed what was already reported by other authors, i.e., that when macrophages are exposed in vitro to nanomaterials, they usually engulf them ( Qie et al, 2016 ; Verçoza et al, 2019 ).…”

Section: Resultssupporting

confidence: 90%

“…We hereby reported that in vitro cultures of macrophages engulfed both bare and antibioticconjugated IONPs. This is not surprising since several studies have shown that macrophages internalize NPs (Verçoza et al, 2019), although, in principle, their surface modifications might allow evasion of phagocytic clearance (Qie et al, 2016). In any case, little is known about the in vivo recruitment of macrophages by nanomaterials and, a fortiori, by nanoantibiotics, depending again on their administration routes, body district distribution and physicochemical properties of their surface.…”

Section: Nanotoxicity Of Gpa-np Systemsmentioning

confidence: 99%

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Antimicrobial Activity of Nanoconjugated Glycopeptide Antibiotics and Their Effect on Staphylococcus aureus Biofilm

et al. 2021

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In the era of antimicrobial resistance, the use of nanoconjugated antibiotics is regarded as a promising approach for preventing and fighting infections caused by resistant bacteria, including those exacerbated by the formation of difficult-to-treat bacterial biofilms. Thanks to their biocompatibility and magnetic properties, iron oxide nanoparticles (IONPs) are particularly attractive as antibiotic carriers for the targeting therapy. IONPs can direct conjugated antibiotics to infection sites by the use of an external magnet, facilitating tissue penetration and disturbing biofilm formation. As a consequence of antibiotic localization, a decrease in its administration dosage might be possible, reducing the side effects to non-targeted organs and the risk of antibiotic resistance spread in the commensal microbiota. Here, we prepared nanoformulations of the ‘last-resort’ glycopeptides teicoplanin and vancomycin by conjugating them to IONPs via surface functionalization with (3-aminopropyl) triethoxysilane (APTES). These superparamagnetic NP-TEICO and NP-VANCO were chemically stable and NP-TEICO (better than NP-VANCO) conserved the typical spectrum of antimicrobial activity of glycopeptide antibiotics, being effective against a panel of staphylococci and enterococci, including clinical isolates and resistant strains. By a combination of different methodological approaches, we proved that NP-TEICO and, although to a lesser extent, NP-VANCO were effective in reducing biofilm formation by three methicillin-sensitive or resistant Staphylococcus aureus strains. Moreover, when attracted and concentrated by the action of an external magnet, NP-TEICO exerted a localized inhibitory effect on S. aureus biofilm formation at low antibiotic concentration. Finally, we proved that the conjugation of glycopeptide antibiotics to IONPs reduced their intrinsic cytotoxicity toward a human cell line.

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Section: Resultssupporting

confidence: 90%

Section: Nanotoxicity Of Gpa-np Systemsmentioning

confidence: 99%

Antimicrobial Activity of Nanoconjugated Glycopeptide Antibiotics and Their Effect on Staphylococcus aureus Biofilm

et al. 2021

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“…Coconut water also finds application in nanotechnology, more particularly in the synthesis of nanoparticles (NPs). Gold [39], silver [40], BaTiO 3 [41], Y 2 O 3 :Nd 3+ [42], and superparamagnetic iron oxide [43] NPs have been synthesized using coconut water.…”

Section: Introductionmentioning

confidence: 99%

Nanostructured LiFe5O8 by a Biogenic Method for Applications from Electronics to Medicine

et al. 2021

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The physical properties of the cubic and ferrimagnetic spinel ferrite LiFe5O8 has made it an attractive material for electronic and medical applications. In this work, LiFe5O8 nanosized crystallites were synthesized by a novel and eco-friendly sol-gel process, by using powder coconut water as a mediated reaction medium. The dried powders were heat-treated (HT) at temperatures between 400 and 1000 °C, and their structure, morphology, electrical and magnetic characteristics, cytotoxicity, and magnetic hyperthermia assays were performed. The heat treatment of the LiFe5O8 powder tunes the crystallite sizes between 50 nm and 200 nm. When increasing the temperature of the HT, secondary phases start to form. The dielectric analysis revealed, at 300 K and 10 kHz, an increase of ε′ (≈10 up to ≈14) with a tanδ almost constant (≈0.3) with the increase of the HT temperature. The cytotoxicity results reveal, for concentrations below 2.5 mg/mL, that all samples have a non-cytotoxicity property. The sample heat-treated at 1000 °C, which revealed hysteresis and magnetic saturation of 73 emu g−1 at 300 K, showed a heating profile adequate for magnetic hyperthermia applications, showing the potential for biomedical applications.

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“…However, despite all their potential, the synthesis processes of the SPIONs are characterized by being expensive and toxic to humans and the environment [6]. In this scenario, our group demonstrated the therapeutic potential of low-cost biocompatible SPIONs produced by green synthesis [18]. Thus, the present study aimed to evaluate in vitro the therapeutic potential of SPIONs produced with coconut water to treat cutaneous leishmaniasis caused by Leishmania amazonensis.…”

Section: Discussionmentioning

confidence: 97%

Green SPIONs as a novel highly selective treatment for leishmaniasis: an in vitro study against Leishmania amazonensis intracellular amastigotes

Verçoza¹,

Bernardo²,

Oliveira³

et al. 2023

Preprint

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Objective: The main goal of this work was to evaluate the therapeutic potential of green SPIONs produced with coconut water for treating cutaneous leishmaniasis caused by Leishmania amazonensis. Materials and methods: Optical and electron microscopy techniques were used to evaluate the effects on cell proliferation, infectivity percentage, and ultrastructure. Results: SPIONs were internalized by both parasite stages, randomly distributed by the cytosol located mainly in membrane-bound compartments. The selectivity index for intracellular amastigotes was higher than 240 times compared to current drugs used to treat the disease. Conclusion: The synthesized SPIONs showed an important and promising anti-Leishmania activity and can be considered a strong candidate for a new therapeutic approach for treating leishmaniases.

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Therapeutic Potential of low-cost Nanocarriers Produced By Green Synthesis: Macrophage Uptake of Superparamagnetic Iron Oxide Nanoparticles

Cited by 17 publications

References 64 publications

Antimicrobial Activity of Nanoconjugated Glycopeptide Antibiotics and Their Effect on Staphylococcus aureus Biofilm

Antimicrobial Activity of Nanoconjugated Glycopeptide Antibiotics and Their Effect on Staphylococcus aureus Biofilm

Nanostructured LiFe5O8 by a Biogenic Method for Applications from Electronics to Medicine

Green SPIONs as a novel highly selective treatment for leishmaniasis: an in vitro study against Leishmania amazonensis intracellular amastigotes

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