Therapeutic Potential of Biochanin-A Against Isoproterenol-Induced Myocardial Infarction in Rats

Abstract

Background: This study determined the effect of Biochanin A (BCA) on isoproterenol (ISO) induced Myocardial Infarction (MI) in male Wistar rats. Methods: Animals (weighing 150-180 g) were divided into four groups, with six animals in each group and pretreated with BCA (10mg/kg Body Weight [BW]) and ɑ-tocopherol (60mg/kg BW) for 30 days; and ISO (20mg/kg BW) was administrated subcutaneously on the 31st and 32nd day. Results: ISO-induced MI rats demonstrated the significant elevation of serum glutamic oxaloacetic transaminase, serum glutamic pyruvic transaminase, lactate dehydrogenase, creatine kinase-MB and cardiac troponin; however, concomitant pretreatment with BCA protected the rats from cardiotoxicity caused by ISO. Activities of antioxidant enzymes, such as superoxide dismutase, catalase, glutathione peroxidase, glutathione-S-transferase and glutathione reductase significantly reduced in the heart with ISO-induced MI. Pretreatment with BCA produced a marked reversal of these antioxidant enzymes related to MI-induced by ISO. Conclusion: In conclusion, this study suggested that BCA exerts cardioprotective effects through modulating lipid peroxidation, enhancing antioxidants, and detoxifying enzyme systems.

Red Propolis and Its Dyslipidemic Regulator Formononetin: Evaluation of Antioxidant Activity and Gastroprotective Effects in Rat Model of Gastric Ulcer

“…The therapeutic potential of biochanin-A against myocardial infarction has also been proposed, and attributed to its capacity in compromising lipid peroxidation. Male Wistar rats were first subjected to isoproterenol-induced myocardial infarction [ 68 ], resulting in a significant increase of creatine kinase-MB and cardiac troponin, serum glutamic oxaloacetic transaminase, serum glutamic pyruvic transaminase, and lactate dehydrogenase, and reduction of the activity of antioxidant enzymes (i.e., SOD, catalase, glutathione peroxidase, glutathione-S-transferase, and glutathione reductase) in the heart. Such antioxidant enzymes catalyze reactions to counterbalance free radicals and ROS, thus forming endogenous defense mechanisms.…”

“…Such antioxidant enzymes catalyze reactions to counterbalance free radicals and ROS, thus forming endogenous defense mechanisms. The authors reported the cardioprotective effects of biochanin-A by modulation of lipid peroxidation, through enhancing antioxidants and detoxifying enzyme systems [ 68 ]. The effect of biochanin-A on a high-fat diet-induced hyperlipidemia in mice has been studied by Xue et al [ 69 ].…”

Phenolic fraction extracted from Kedrostis foetidissima leaves ameliorated isoproterenol‐induced cardiotoxicity in rats through restoration of cardiac antioxidant status

“…Changes in the serum levels of creatine kinase‐muscle/brain (CK‐MB), LDH, serum glutamic oxaloacetic transaminase (SGOT), and troponin‐T could help in the assessment of MI‐related cardiac injury (Hemalatha & Prince, 2015). Cytosolic enzymes, such as ALT, AST, LDH, and CK, released as a result of ISO administration into bloodstream indicated the cytotoxic property of ISO (Govindasami et al., 2020). The levels of these cellular enzymes point out the alteration in permeability or integrity of the plasma membrane (Hassan, Akhtar, Ahmed, Ahmad, & Najmi, 2017).…”

“…ISO causes imbalance in energy reserve of cardiac muscles and results in a sequence of structural and biochemical changes that contributes to the pathogenesis of cell damage in heart muscles (Mahammad & Lakshmi Devi, 2013). ISO-induced MI rat model is widely used to demonstrate the alterations in hematological, biochemical, and oxidative stress markers in order to validate cardioprotective effects of natural products (Govindasami, Uddandrao, Raveendran, & Sasikumar, 2020;Lobo et al, 2011). Kedrostis foetidissima (Jacq.)…”

Cardioprotection of Sodium–Glucose Cotransporter 2 Inhibition in Rats With Isoproterenol-Induced Cardiomyopathy

“…A recent nationwide population-based longitudinal cohort study revealed that patients with type 2 diabetes prescribed with SGLT2i were associated with a lower risk of all-cause mortality and new-onset arrhythmias compared with those not taking SGLT2i in real-world practice [9]. Isoproterenol (ISO) as a synthetic nonselective β-adrenoceptor agonist is well accepted to induce myocardial damage in rats for evaluating cardiac dysfunctions [10]. The pathophysiological and morphological changes of ISO-induced myocardial changes are similar to those observed in human with myocardial infarction or heart failure.…”