Therapeutic faecal microbiota transplantation controls intestinal inflammation through IL10 secretion by immune cells

Burrello, Claudia; Garavaglia, Federica; Cribiù, Fulvia Milena; Ercoli, Giulia; Lopez, Gianluca; Troisi, Jacopo; Colucci, Angelo; Guglietta, Silvia; Carloni, Sara; Guglielmetti, Simone; Taverniti, Valentina; Nizzoli, Giulia; Bòsari, Silvano; Caprioli, Flavio; Rescigno, María; Facciotti, Federica

doi:10.1038/s41467-018-07359-8

Cited by 205 publications

(201 citation statements)

References 80 publications

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“…Studies have shown that FMT is involved in the low-grade inflammation characterized by metabolic disorders. It has been reported that therapeutic FMT could decrease the secretion of inflammatory factors and triggered several immune-mediated signal-pathways in colitis (Burrello et al, 2018). As mentioned in another study, transplantation of gut microbiota like F. prausnitzii prevented the inflammation damage in the pancreas (Ganesan et al, 2018).…”

Section: Discussionmentioning

confidence: 80%

“…In our study, we established T2DM animal models by feeding mice high-fat diet combined with streptozotocin and observed that FMT treatment successfully reduced FBG and improved glucose tolerance on diabetic mice (Figures 1A,B). It was possible to restore the balance of intestinal microflora to promote host homeostasis (Burrello et al, 2018). According to a recent study, in metabolic syndrome, when commensal bacteria from various phyla stay in a certain range, they contribute to control the ratio of pathogenic species and support optimal physical conditions (Burrello et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

“…It was possible to restore the balance of intestinal microflora to promote host homeostasis (Burrello et al, 2018). According to a recent study, in metabolic syndrome, when commensal bacteria from various phyla stay in a certain range, they contribute to control the ratio of pathogenic species and support optimal physical conditions (Burrello et al, 2018). Glycated hemoglobin test usually reflects the patient's blood glucose control in the last 8-10 weeks and our ELISA results of HbA1c further provided evidence of the hypoglycemic effect of FMT ( Figure 1C).…”

Section: Discussionmentioning

confidence: 99%

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Promising Treatment for Type 2 Diabetes: Fecal Microbiota Transplantation Reverses Insulin Resistance and Impaired Islets

Wang

Lei

Yan

et al. 2020

Front. Cell. Infect. Microbiol.

137

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Type 2 diabetes is a common metabolic disorder related to insulin resistance, or deficiency of insulin secretion, caused by decreased insulin sensitivity and destruction of islet structure and function. As the second human genome, the microbiota has been observed to have a growing relationship with diabetes in recent years. Microbiota imbalance has been hypothesized to be involved in the regulation of energy metabolism and the inflammatory immune response in diabetes. The present study aimed to investigate whether fecal microbiota transplantation (FMT) could alleviate the symptoms associated with type 2 diabetes. To this end, a type 2 diabetes mouse model was first established through the consumption of a high-fat diet combined with streptozotocin (100 mg/kg), and FMT was used to rebuild the gut microbiota of diabetic mice. Fasting blood glucose, oral glucose tolerance tests, and HbA1c levels were monitored, while the hypoglycemic effects of FMT were also observed. Insulin levels were tested by ELISA and related indexes such as HOMA-IR, HOMA-IS, and HOMA-β were calculated. We found that insulin resistance and pancreatic islet β-cells were improved after FMT treatment. Meanwhile, the markers of inflammation in the pancreatic tissue were detected by ELISA and immunohistochemistry, which indicated that inflammatory response decreased following FMT treatment. Furthermore, flow cytometry and western blot results revealed that FMT inhibited the β-cell apoptosis. Here, the effect of FMT on hypoglycemia in type 2 diabetes was addressed by improving insulin resistance and repairing impaired islets, thereby providing a potential treatment strategy for type 2 diabetes.

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Section: Discussionmentioning

confidence: 80%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Promising Treatment for Type 2 Diabetes: Fecal Microbiota Transplantation Reverses Insulin Resistance and Impaired Islets

Wang

Lei

Yan

et al. 2020

Front. Cell. Infect. Microbiol.

137

View full text Add to dashboard Cite

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“…Recently, experimental evidence has linked the effectiveness of FMT for colitis with immunosuppression. FMT therapeutically controlled gut inflammation and colitis via the induction of IL-10 and TGF-β, cytokines critical for Treg cell accumulation in the intestine [67,68]. Whether FMT-directed immunosuppression aids in the recovery from C. difficile requires further investigation.…”

Section: Certain Microbes Can Elicit Immunosuppression After Colonizamentioning

confidence: 99%

Considering the Immune System during Fecal Microbiota Transplantation for Clostridioides difficile Infection

Frisbee

Petri

2020

Trends in Molecular Medicine

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Our understanding and utilization of fecal microbiota transplantation (FMT) has jump-started over the past two decades. Recent technological advancements in sequencing and metabolomics have allowed for better characterization of our intestinal microbial counterparts, triggering a surge of excitement in the fields of mucosal immunology and microbiology. This excitement is well founded, as demonstrated by 90% relapse-free cure rates in FMT treatment for recurrent Clostridioides difficile infections. Growing evidence suggests that in addition to bacterial factors, the host immune response during C. difficile infection greatly influences disease severity. In this review, we discuss recent advancements in understanding the interplay between immune cells and the microbiota and how they may relate to recovery from C. difficile through FMT therapy.

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“…While the mechanism has not been established in humans, it is likely to involve microbial activation of innate immune responses through pattern recognition receptors on macrophages and DCs, leading to activation of pathogenic Th1/Th17 cells. Studies in the DSS‐induced murine colitis model have shown that therapeutic FMT promotes IL‐10‐dependent suppression of APC and CD4 T cells that mediate colon inflammation [60]. Therefore, FMT in humans may also function through introduction of bacteria to the gut that promote regulatory immune responses, which suppress colon inflammation mediated by innate immune cells and T cells.…”

Section: Introductionmentioning

confidence: 99%

The microbiota and immune‐mediated diseases: Opportunities for therapeutic intervention

Fitzgibbon

Mills

2020

Eur J Immunol

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A multitude of diverse microorganisms, termed the microbiota, reside in the gut, respiratory tract, skin, and genital tract of humans and other animals. Recent advances in metagenomic sequencing and bioinformatics have enabled detailed characterization of these vital microbial communities. Studies in animal models have uncovered vital previously unrecognized roles for the microbiota in normal function of the immune responses, and when perturbed, in the pathogenesis of diseases of the gastrointestinal tract and lungs, but also at distant sites in the body including the brain. The composition of gut and respiratory microbiota can influence systemic inflammatory responses that mediate asthma, allergy, inflammatory bowel disease, obesity‐related diseases, and neurodevelopmental or neurodegenerative conditions. Experiments in mouse models as well as emerging clinical studies have revealed that therapeutic manipulation of the microbiota, using fecal microbiota transplantation, probiotics, or engineered probiotics represent effective nontoxic approaches for the treatment or prevention of Clostridium difficile infection, allergy, and autoimmune diseases and may enhance the efficacy of certain cancer immunotherapeutics. This review discusses how commensal bacteria can influence immune responses that mediate a range of human diseases and how the microbiota are being targeted to treat these diseases, especially those resistant to pharmacological therapies.

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Therapeutic faecal microbiota transplantation controls intestinal inflammation through IL10 secretion by immune cells

Cited by 205 publications

References 80 publications

Promising Treatment for Type 2 Diabetes: Fecal Microbiota Transplantation Reverses Insulin Resistance and Impaired Islets

Promising Treatment for Type 2 Diabetes: Fecal Microbiota Transplantation Reverses Insulin Resistance and Impaired Islets

Considering the Immune System during Fecal Microbiota Transplantation for Clostridioides difficile Infection

The microbiota and immune‐mediated diseases: Opportunities for therapeutic intervention

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