Therapeutic Enzymes

Vijayan, Nimisha; Swapna, T. S.; Haridas, M.; Sabu, Abdulhameed

doi:10.1016/b978-0-444-63662-1.00011-7

Cited by 7 publications

(2 citation statements)

References 35 publications

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“…Producing extracellular enzymes which are needed to break down organic material for sustainable soil productivity. L-glutaminase plays a major role in the nitrogen metabolism of both prokaryotes and eukaryotes [2] . It is an important enzyme therapy for a variety disease of the lymphatic system especially acute lymphocytic leukemia [3] .…”

Section: Introductionmentioning

confidence: 99%

Purification of L-Glutaminase from Bacillus sp. B12 and study its properties

Abdelhameed¹,

Alqaysi²,

Hilal³

et al. 2020

IJFMT

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L-glutaminase is an enzyme that catalyse the conversion L-glutamine to glutamic acid and ammonia. The important application of the L-glutaminase is a chemotherapy agent. In this study a novel strain, Bacillus B12. that isolated from Diyala soil was explored for production of extracellular L-glutaminase. The enzyme has been purified 23.9-fold from cell-free extract with 45.4% recovery ( specific activity 76.6 U/mg protein). Enzyme has molecular weight of 199 kDa, and pH 8 and stable in pH range 6-9.5. Temperature optimum is 40 ⁰C and completely stable between 25-45 ⁰C. The kinetics studies revealed that the km and V max of purified L-glutaminase were estimated in 0.4mmol/L and 0.133mmol/min, respectively. The result showed that, the enzyme was active when incubated with 10 mM of Mn 2+ , Mg 2+ ,Ca +2 and Na + . Whereas, K + , Co 2+ , and Ni 2+ show no effect. However, Hg 2+ , Cu 2+ ,Fe 2+ and Zn 2+ decreased L-glutaminase activity . The 10mM of DEAE, PMSF, and sodium azide did not show a clear effect against the enzyme activity. Higher decrease in enzyme activity was observed by using Cysteine and 2-Mercaptoethanol ( 30 and 25%, respectively).

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Section: Introductionmentioning

confidence: 99%

Purification of L-Glutaminase from Bacillus sp. B12 and study its properties

Abdelhameed¹,

Alqaysi²,

Hilal³

et al. 2020

IJFMT

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show abstract

“…Glutaminase is an inner mitochondrial membrane enzyme predominant in the brain . In neurons, glutaminase is the predominant glutamine‐utilizing and glutamate‐producing enzyme; hence, glutaminase has the potential to induce neurotoxicity by elevating glutamate .…”

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confidence: 99%