L-glutaminase is an enzyme that catalyse the conversion L-glutamine to glutamic acid and ammonia. The important application of the L-glutaminase is a chemotherapy agent. In this study a novel strain, Bacillus B12. that isolated from Diyala soil was explored for production of extracellular L-glutaminase. The enzyme has been purified 23.9-fold from cell-free extract with 45.4% recovery ( specific activity 76.6 U/mg protein). Enzyme has molecular weight of 199 kDa, and pH 8 and stable in pH range 6-9.5. Temperature optimum is 40 ⁰C and completely stable between 25-45 ⁰C. The kinetics studies revealed that the km and V max of purified L-glutaminase were estimated in 0.4mmol/L and 0.133mmol/min, respectively. The result showed that, the enzyme was active when incubated with 10 mM of Mn 2+ , Mg 2+ ,Ca +2 and Na + . Whereas, K + , Co 2+ , and Ni 2+ show no effect. However, Hg 2+ , Cu 2+ ,Fe 2+ and Zn 2+ decreased L-glutaminase activity . The 10mM of DEAE, PMSF, and sodium azide did not show a clear effect against the enzyme activity. Higher decrease in enzyme activity was observed by using Cysteine and 2-Mercaptoethanol ( 30 and 25%, respectively).
Fertilization need increased the concentration of Ca + for egg activation. Inositol 1,4,5trisphosphate (IP3), the second messenger of phospholipase Cγ, regulates the initial calcium rise from egg's endoplasmic reticulum. However, FAK (focal adhesion kinase) is a non-receptor tyrosine kinase, which is known to create docking sites for signaling molecules that contain SH2 domains, including PLC. PLC participates in other signaling pathways besides fertilization and the role FAK may play in fertilization has not been determined. The goal of these experiments is to determine is the FAK is the link between Src and PLC, as well as, confirming or not confirming if Src directly or indirectly activates PLC.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
customersupport@researchsolutions.com
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.