Therapeutic effects of thymoquinone or capsaicin on acrylamide-induced reproductive toxicity in rats mediated by their effect on oxidative stress, inflammation, and tight junction integrity

Haleem, Ekram Nemr Abd Al; Hasan, Walaa Yousef Soliman; Arafa, Hossam M.M.

doi:10.1080/01480545.2021.1942485

Cited by 13 publications

(10 citation statements)

References 59 publications

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“…We thought that this decrease is due to testicular damage. In a study, it has been observed that Thymoquinone and Capsaicin administration inhibited the elimination of ACR‐induced reproductive hormone disorders 35 . Similar to the findings obtained in this study, we determined that serum FHS, LH, and dihydrotestosterone levels were normalized in the groups treated with especially high doses of NA.…”

Section: Discussionsupporting

confidence: 88%

“…In a study, it has been observed that Thymoquinone and Capsaicin administration inhibited the elimination of ACR-induced reproductive hormone disorders. 35 Similar to the findings obtained in this study, we determined that serum FHS, LH, and dihydrotestosterone levels were normalized in the groups treated with especially high doses of NA. This is an important indication that NA inhibits ACR-induced damage to testicular tissue and hormonal disorders.…”

Section: Discussionsupporting

confidence: 88%

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Effects of naringin on oxidative stress, inflammation, some reproductive parameters, and apoptosis in acrylamide‐induced testis toxicity in rat

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Yıldırım

et al. 2023

Environmental Toxicology

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Acrylamide (ACR) is used in many fields such as cosmetics, paper, and textile industries. It also occurs at very high temperatures in some foods. Gonadotoxic effects of ACR have been found in experimental animals. Many studies use flavonoids to prevent the reproductive side effects of ACR. Naringin (NA) is a flavonoid and it has been determined by studies that it has no toxic effect on tissues. In our study, we aimed to determine the protective effect of NA against the damage of ACR on testicular tissue and the reproductive system in rats. In our study, 50 Spraque Dawley male rats weighing 220‐250 grams were used. Control: Only intragastric saline was administered for 10 days. ACR: Animals received ACR (40 mg/kg, intraperitoneally) for 10 days. NA50+ACR: Animals were given NA for 10 days and each NA was one hour after the administration of ACR. NA100+ACR: Animals received NA for 10 days and one hour after each NA was given ACR. NA100: Animals were given NA for 10 days. At the end of the applications, the rats were euthanized by cervical dislocation under anesthesia. Serum FSH, LH, and Dihydrotestosterone levels were compared between the groups. In addition, oxidative stress, inflammation, expression of some reproductive enzymes, and apoptosis markers were determined in testicular tissues. When these parameters were compared between groups, ACR induced testicular dysfunction and tissue damage in rats. We determined that only the NA application did not cause tissue damage. and the administration of NA along with ACR significantly reduced ACR‐induced testis toxicity.

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Section: Discussionsupporting

confidence: 88%

Section: Discussionsupporting

confidence: 88%

Effects of naringin on oxidative stress, inflammation, some reproductive parameters, and apoptosis in acrylamide‐induced testis toxicity in rat

Şengül

Gelen

Yıldırım

et al. 2023

Environmental Toxicology

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“…Capsaicin administration effectively counteracted the inflammatory effects induced by Bleomycin by reducing the expression of TNF-α, NF-κB, TGF-β1 and COX-2, as well as decreasing NO production. These results align with previous studies demonstrating Capsaicin’s anti-inflammatory effects against cyclophosphamide and acrylamide toxicities, as elucidated by Melekoglu et al (2018) ; Abd Al Haleem et al (2022) .…”

Section: Discussionsupporting

confidence: 92%

Capsaicin ameliorate pulmonary fibrosis via antioxidant Nrf-2/ PPAR- γ pathway activation and inflammatory TGF-β1/ NF-κB/COX II pathway inhibition

Abdulaal,

Asfour,

Helmi

et al. 2024

Front. Pharmacol.

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Bleomycin is an effective antibiotic with a significant anticancer properties, but its use is limited due to its potential to induce dose-dependent pulmonary fibrosis. Therefore, this study aimed to assess the therapeutic potential of Capsaicin as an additional treatment to enhance patient tolerance to Bleomycin compared to the antifibrotic drug Pirfenidone. Pulmonary fibrosis was induced in rats through by a single intratracheal Bleomycin administration in day zero, followed by either Capsaicin or Pirfenidone treatment for 7 days. After the animals were sacrificed, their lungs were dissected and examined using various stains for macroscopic and histopathological evaluation. Additionally, the study assessed various antioxidant, anti-inflammatory, and antifibrotic parameters were assessed. Rats exposed to Bleomycin exhibited visible signs of fibrosis, histopathological alterations, increased collagen deposition, and elevated mucin content. Bleomycin also led to heightened increased inflammatory cells infiltration in the bronchoalveolar lavage, elevated fibrosis biomarkers such as hydroxyproline, alpha-smooth muscle actin (α-SMA) and transforming growth factor-beta (TGF-β1), increased inflammatory markers including tumor necrosis factor-alpha (TNF-α), interlukine-6 (Il-6), interlukine-1β (Il-1β) nuclear factor-kappa B (NF-κB), and Cyclooxygenase-2 (COX-2), and transforming growth factor-beta (TGF-β1),. Furthermore, it reduced the expression of peroxisome proliferator-activated receptor-gamma (PPAR-γ), increased oxidative stress biomarkers like nitric oxide (NO), malondialdehyde (MDA), myeloperoxidase (MPO) and protein carbonyl. Bleomycin also decreased the expression of nuclear factor erythroid 2–related factor 2 (Nrf-2), reduced glutathione (GSH), total antioxidant capacity, and the activities of catalase and superoxide dismutase (SOD). Treating the animals with Capsaicin and Pirfenidone following Bleomycin exposure resulted in improved lung macroscopic and microscopic characteristics, reduced collagen deposition (collagen I and collagen III) and mucin content, decreased inflammatory cell infiltration, lowered levels of hydroxyproline, α-SMA, and TGF-β1, decreased TNF-α, Il-6, Il-1β, NF-κB, and COX-2, increased PPAR-γ and Nrf-2 expression, and improvement improved in all oxidative stress biomarkers. In summary, Capsaicin demonstrates significant antifibrotic activity against Bleomycin-induced lung injury that may be attributed, at least in part, to the antioxidant and anti-inflammatory activities of Capsaicin mediated by upregulation of PPAR-γ and Nrf-2 expression and decreasing. TGF-β1, NF-κB and COX II proteins concentrations.

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“…ACR is also present in cigarette smoke and carbohydrate-rich food ingredients processed by high-temperature frying or baking, such as fries, potato chips, coffee, and bread, as a product of Maillard reactions [ 2 ]. Due to its neurotoxicity [ 3 ], reproductive toxicity [ 4 ], genotoxicity, and carcinogenicity to animals [ 5 ], ACR has been classified as “reasonably anticipated to be a human carcinogen” by the National Toxicology Program and as a group 2A carcinogen by the International Agency for Research on Cancer [ 6 ]. Among the types of ACR-induced toxicity, neurotoxicity is the earliest to present and has been studied the most thoroughly.…”

Section: Introductionmentioning

confidence: 99%

AQP4 Attenuated TRAF6/NFκB Activation in Acrylamide-Induced Neurotoxicity

et al. 2022

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Acrylamide (ACR) is present in high-temperature-processed high-carbohydrate foods, cigarette smoke, and industrial pollution. Chronic exposure to ACR may induce neurotoxicity from reactive oxygen species (ROS); however, the mechanisms underlying ACR-induced neurotoxicity remain unclear. We studied 28-day subacute ACR toxicity by repeatedly feeding ACR (0, 15, or 30 mg/kg) to rats. We conducted RNA sequencing and Western blot analyses to identify differences in mRNA expression in the blood and in protein expression in the brain tissues, respectively, of the rats. AQP4 transient transfection was performed to identify potential associations with protein regulation. The rats treated with 30 mg/kg ACR exhibited hind-limb muscle weakness. Matrix metalloproteinase (MMP9) expression was higher in the ACR-treated group than in the control group. ACR induced MMP-9 and AQP4 protein expression in the brain tissues of the rats, which subsequently presented with neurotoxicity. In the in vitro study, Neuro-2a cells were transiently transfected with AQP4, which inhibited MMP-9 and TNF receptor-associated factor 6 (TRAF6) expression, and inhibited ACR induced expression of TRAF6, IκBα, and nuclear factor κB (NFκB). Using a combination of in vivo and in vitro experiments, this study revealed that depressive symptoms associated with ACR-induced neurotoxicity are associated with downregulation of AQP4 and induction of the TRAF6 pathway.

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Therapeutic effects of thymoquinone or capsaicin on acrylamide-induced reproductive toxicity in rats mediated by their effect on oxidative stress, inflammation, and tight junction integrity

Cited by 13 publications

References 59 publications

Effects of naringin on oxidative stress, inflammation, some reproductive parameters, and apoptosis in acrylamide‐induced testis toxicity in rat

Effects of naringin on oxidative stress, inflammation, some reproductive parameters, and apoptosis in acrylamide‐induced testis toxicity in rat

Capsaicin ameliorate pulmonary fibrosis via antioxidant Nrf-2/ PPAR- γ pathway activation and inflammatory TGF-β1/ NF-κB/COX II pathway inhibition

AQP4 Attenuated TRAF6/NFκB Activation in Acrylamide-Induced Neurotoxicity

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