Therapeutic effects of rectal administration of basic fibroblast growth factor on experimental murine colitis

Matsuura, Minoru; Okazaki, Kazuichi; Nishio, Akiyoshi; Nakase, Hiroshi; Tamaki, Hiroyuki; Uchida, Kazushige; Nishi, Tatsushi; Asada, Masanori; Kawasaki, Kohkichi; Fukui, Toshiro; Yoshizawa, Hazuki; Ohashi, Shinya; Inoue, Satoko; Kawanami, Chiharu; Hayashi, Hiroshi; Tabata, Yasuhiko; Chiba, Tsutomu

doi:10.1053/j.gastro.2005.01.006

Cited by 66 publications

(49 citation statements)

References 59 publications

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“…EGF is involved in the maintenance of mucosal integrity and is also a potent mitogen of the gastric epithelium; it stimulates mucin production and enhances prostaglandin synthesis. [27,28] In the present case, neither skin toxicity nor intestinal toxicity was experienced with erlotinib treatment.…”

Section: Case Reportcontrasting

confidence: 46%

Medical Treatment with Two Targeted Therapies for an Oncology Patient with Double Primary Cancer: Case Report

Namal¹

2016

TJ Oncol

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SUMMARYWhile multiple primary tumors are rarely reported in literature, we expect that it will be seen more often in the future as a result of improvements in diagnosis and treatment of cancer. Molecular targeted therapies are promising modalities that are now used more commonly. Presently described is oncological response for a 70-year-old women with multiple primary cancers -breast and lung -in which 2 targeted therapies were administered. While there is currently no standard therapy in the literature for multiple primary cancers, it is the authors' suggestion that targeted therapies may be a promising choice for these cases.

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Section: Case Reportcontrasting

confidence: 46%

Medical Treatment with Two Targeted Therapies for an Oncology Patient with Double Primary Cancer: Case Report

Namal¹

2016

TJ Oncol

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“…Thus, it is not astonishing that in a randomized, double-blind clinical trial, after a 2-wk treatment period, patients receiving EGF enemas had a significant lower disease activity score than the control patients [51] . Concerning basic FGF, in a mouse DSS-model of experimental colitis, rectal administration of human recombinant basic FGF ameliorated the inflammation score and suppressed TNF-α gene expression in the colonic tissue [52,53] . Recently, the important role of Glucagon-like-peptide-2 (GLP-2), which is secreted from local neuroendocrine epithelial cells and promotes epithelial cell proliferation via stimulation of enteric neurons [54] , has received more attention.…”

Section: The Role Of Regulatory Peptides For Intestinal Epithelial Womentioning

confidence: 99%

Epithelial restitution and wound healing in inflammatory bowel disease

Sturm¹,

Dignaß²

2008

WJG

288

260

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disorders including inflammatory bowel diseases and require constant repair of the epithelium. Enhancement of intestinal repair mechanisms by regulatory peptides or other modulatory factors may provide future approaches for the treatment of diseases that are characterized by injuries of the epithelial surface. THE MUCOSAL DEFENSE SYSTEMThe surface of the digestive tract is covered by epithelial cells that constitute an efficient physical barrier between the dietary and enteric flora pathogens found in the intestinal lumen and the individuum, but also allows an exchange between nutrients and the systemic circulation [1] . The epithelial defense mechanism can be categorized into three key components: pre-epithelial, epithelial and post-epithelial, the latter is represented by the lamina propria [2] . The pre-epithelial mucus barrier is composed of mucin associated with other proteins and lipids and forms a continuous gel into which a bicarbonate-rich fluid is secreted, maintaining a neutralizing pH at the epithelial surface. Phosphatidylcholine is the predominant surface bioactive phospholipid found within the gastrointestinal tract [3] . Intestinal epithelial cells secrete mucins and glycocalyx, which contain membrane-anchored negatively charged mucin-like glycoproteins and hydrophobic phospholipids [4] . The tight adherence of mucin to the apical surfaces of epithelia is owed to the existence of the specific complex between mucin oligosaccharides and the mucin binding protein of the apical mucosal membrane [5] . AbstractInflammatory bowel disease is characterized by a chronic inflammation of the intestinal mucosa. The mucosal epithelium of the alimentary tract constitutes a key element of the mucosal barrier to a broad spectrum of deleterious substances present within the intestinal lumen including bacterial microorganisms, various dietary factors, gastrointestinal secretory products and drugs. In addition, this mucosal barrier can be disturbed in the course of various intestinal disorders including inflammatory bowel diseases. Fortunately, the integrity of the gastrointestinal surface epithelium is rapidly reestablished even after extensive destruction. Rapid resealing of the epithelial barrier following injuries is accomplished by a process termed epithelial restitution, followed by more delayed mechanisms of epithelial wound healing including increased epithelial cell proliferation and epithelial cell differentiation. Restitution of the intestinal surface epithelium is modulated by a range of highly divergent factors among them a broad spectrum of structurally distinct regulatory peptides, variously described as growth factors or cytokines. S e ve ra l re g u l a t or y pe pt ide fac t or s ac t from t he basolateral site of the epithelial surface and enhance epithelial cell restitution through TGF-β-dependent pathways. In contrast, members of the trefoil factor family (TFF peptides) appear to stimulate epithelial restitution in conjunction with mucin glycoproteins through a TGF-β-independent mechanism from...

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“…Total RNA was extracted from 5 × 10 5 sorted cells and purified using the RNeasy kit according to the manufacturer's instructions (Qiagen, Valencia, CA) and converted to cDNA by moloney murine leukemia virus (M-MLV) reverse transcriptase with oligo(dT) primer in the presence of RNAsin (Promega, Madison, WI). Semi-quantitative PCR for Foxp3, TGF-β 1 , IL-10, CCR1, CCR5, CCR6, CCR7, and β-actin was perfor med as described previously [24][25][26][27][28] . The cDNA generated was subjected to 30-35 cycles of PCR amplification on a DNA thermal cycler (Perkin Elmer, GeneAmp PCR System).…”

Section: Semi-quantitative and Real-time Pcrmentioning

confidence: 99%

“…The primer and TaqMan probe sequences used as previously described [26] are as follows: Foxp3 primers (5'-CCCAGGAAAGACAGCAACCTT-3' and 5'-TTCTCACAACCAGGCCACTTG-3'), Foxp3 probe (5'-FAM-ATCCTACCCACTGCTGGCAAATGG AGTC-3'), HPRT primers (5'-TGAAGAGCTACTGTA ATGATCAGTCAAC-3' and 5'-AGCAAGCTTGCA ACCTTAACCA-3'), HPRT probe (5'-FAM-TGCTTT CCCTGGTTAAGCAGTACAGCCC-3'). Standard curves of cDNAs from ICR mice CD4 + CD25 + T cells were used as previously described [27] . The normalized values for Foxp3 mRNA were calculated as the relative quantity of Foxp3 mRNA levels divided by the relative quantity of HPRT mRNA levels.…”

Section: Semi-quantitative and Real-time Pcrmentioning

confidence: 99%

Depletion of CD4+CD25+ regulatory T cells can promote local immunity to suppress tumor growth in benzo[a]pyrene-induced forestomach carcinoma

Chen¹,

Fang²,

Lai³

et al. 2008

WJG

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AIM:To elucidate the distribution of CD4 + CD25 + regulatory T cells (Tregs) in different lymphoid tissues and its local enhancement on tumor growth before and after depletion of CD4 + CD25 + Tregs. METHODS: Female ICR mice were gavaged with b e n z o [ a ] p y r e n e ( B a P ) t o i n d u c e fo r e s t o m a c h carcinoma. CD4 + CD25 + Tregs were intraperitoneally depleted with monoclonal antibody PC61. These mice were divided into BaP-only, BaP + IgG, BaP + PC61, and control groups. The forestomach of mice was dissected for histological analysis, and tunnel test was performed for apoptosis of tumor cells. CD4 + CD25 + Tregs were sorted from different lymphoid tissues and expression of Foxp3, IL-10, and chemokine receptors was analyzed by flow cytometry, semi-quantitative and real-time polymerase chain reaction. RESULTS: The mice gavaged with only BaP showed increased forestomach papilloma and carcinoma at wk 16 and 32. The proportion of CD4 + CD25 + Tregs was significantly higher in peri-stomach regional lymph nodes than in other lymphoid tissues. These CD4 + CD25 + Tregs in regional lymph nodes expressed higher levels of Foxp3 and IL-10, enriched in the CD62L-subset, and CCR1 and CCR5 chemokine re c e pt or s . In mic e gavage d w i t h B aP + P C 6 1 , the number of tumor nodules and tumor volume decreased significantly with massive infiltrating cells and apoptosis of tumor cells. In the draining regional lymph nodes, the number of CD4 + CD25 + Tregs also decreased significantly.

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Therapeutic effects of rectal administration of basic fibroblast growth factor on experimental murine colitis

Abstract: Rectal administration of bFGF might be a promising option for the treatment of inflammatory bowel disease.

Cited by 66 publications

References 59 publications

Medical Treatment with Two Targeted Therapies for an Oncology Patient with Double Primary Cancer: Case Report

Medical Treatment with Two Targeted Therapies for an Oncology Patient with Double Primary Cancer: Case Report

Epithelial restitution and wound healing in inflammatory bowel disease

Depletion of CD4+CD25+ regulatory T cells can promote local immunity to suppress tumor growth in benzo[a]pyrene-induced forestomach carcinoma

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