2005
DOI: 10.1053/j.gastro.2005.01.006
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Therapeutic effects of rectal administration of basic fibroblast growth factor on experimental murine colitis

Abstract: Rectal administration of bFGF might be a promising option for the treatment of inflammatory bowel disease.

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Cited by 66 publications
(49 citation statements)
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References 59 publications
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“…EGF is involved in the maintenance of mucosal integrity and is also a potent mitogen of the gastric epithelium; it stimulates mucin production and enhances prostaglandin synthesis. [27,28] In the present case, neither skin toxicity nor intestinal toxicity was experienced with erlotinib treatment.…”
Section: Case Reportcontrasting
confidence: 46%
“…EGF is involved in the maintenance of mucosal integrity and is also a potent mitogen of the gastric epithelium; it stimulates mucin production and enhances prostaglandin synthesis. [27,28] In the present case, neither skin toxicity nor intestinal toxicity was experienced with erlotinib treatment.…”
Section: Case Reportcontrasting
confidence: 46%
“…Thus, it is not astonishing that in a randomized, double-blind clinical trial, after a 2-wk treatment period, patients receiving EGF enemas had a significant lower disease activity score than the control patients [51] . Concerning basic FGF, in a mouse DSS-model of experimental colitis, rectal administration of human recombinant basic FGF ameliorated the inflammation score and suppressed TNF-α gene expression in the colonic tissue [52,53] . Recently, the important role of Glucagon-like-peptide-2 (GLP-2), which is secreted from local neuroendocrine epithelial cells and promotes epithelial cell proliferation via stimulation of enteric neurons [54] , has received more attention.…”
Section: The Role Of Regulatory Peptides For Intestinal Epithelial Womentioning
confidence: 99%
“…Total RNA was extracted from 5 × 10 5 sorted cells and purified using the RNeasy kit according to the manufacturer's instructions (Qiagen, Valencia, CA) and converted to cDNA by moloney murine leukemia virus (M-MLV) reverse transcriptase with oligo(dT) primer in the presence of RNAsin (Promega, Madison, WI). Semi-quantitative PCR for Foxp3, TGF-β 1 , IL-10, CCR1, CCR5, CCR6, CCR7, and β-actin was perfor med as described previously [24][25][26][27][28] . The cDNA generated was subjected to 30-35 cycles of PCR amplification on a DNA thermal cycler (Perkin Elmer, GeneAmp PCR System).…”
Section: Semi-quantitative and Real-time Pcrmentioning
confidence: 99%
“…The primer and TaqMan probe sequences used as previously described [26] are as follows: Foxp3 primers (5'-CCCAGGAAAGACAGCAACCTT-3' and 5'-TTCTCACAACCAGGCCACTTG-3'), Foxp3 probe (5'-FAM-ATCCTACCCACTGCTGGCAAATGG AGTC-3'), HPRT primers (5'-TGAAGAGCTACTGTA ATGATCAGTCAAC-3' and 5'-AGCAAGCTTGCA ACCTTAACCA-3'), HPRT probe (5'-FAM-TGCTTT CCCTGGTTAAGCAGTACAGCCC-3'). Standard curves of cDNAs from ICR mice CD4 + CD25 + T cells were used as previously described [27] . The normalized values for Foxp3 mRNA were calculated as the relative quantity of Foxp3 mRNA levels divided by the relative quantity of HPRT mRNA levels.…”
Section: Semi-quantitative and Real-time Pcrmentioning
confidence: 99%