Therapeutic Effects of Human Umbilical Cord-Derived Mesenchymal Stem Cells in Acute Lung Injury Mice

Abstract

The incidence and mortality of acute lung injury (ALI)/acute respiratory distress syndrome (ARDS) are still very high, but stem cells show some promise for its treatment. Here we found that intratracheal administration of human umbilical cord-mesenchymal stem cells (UC-MSCs) significantly improved survival and attenuated the lung inflammation in lipopolysaccharide (LPS)-induced ALI mice. We also used the proteins-chip and bioinformatics to analyze interactions between UC-MSCs treatment and immune-response alternations of ALI mice. Then we demonstrated that UC-MSCs could inhibit the inflammatory response of mouse macrophage in ALI mice, as well as enhance its IL-10 expression. We provide data to support the concept that the therapeutic capacity of UC-MSCs for ALI was primarily through paracrine secretion, particularly of prostaglandin-E2 (PGE2). Furthermore, we showed that UC-MSCs might secrete a panel of factors including GM-CSF, IL-6 and IL-13 to ameliorate ALI. Our study suggested that UC-MSCs could protect LPS-induced ALI model by immune regulation and paracrine factors, indicating that UC-MSCs should be a promising strategy for ALI/ARDS.

Mesenchymal stem cells: Cell therapy and regeneration potential

“…In a recent report, MSCs were intratracheally administrated into mice with an acute lung injury that caused inflammation. These MSCs demonstrated an inhibition of the inflammatory response of the macrophages by secreting prostaglandin‐E2, granulocyte‐macrophage colony‐stimulating factor (GM‐CSF), interleukin 6 (IL‐6), and interleukin 13 (IL‐13) and showed improvement in the diseased lungs (Zhu et al, ). Together, these new developments in the field of regenerative medicine have provided stimulus for the use of MSCs in clinical studies.…”

“…Results with * p ≤ .05, ** p ≤ .01 were considered statistically significant [Colour figure can be viewed at wileyonlinelibrary.com] an acute lung injury that caused inflammation. These MSCs demonstrated an inhibition of the inflammatory response of the macrophages by secreting prostaglandin-E2, granulocyte-macrophage colonystimulating factor (GM-CSF), interleukin 6 (IL-6), and interleukin 13 (IL-13) and showed improvement in the diseased lungs(Zhu et al, 2017). Together, these new developments in the field of regenerative medicine have provided stimulus for the use of MSCs in clinical studies.Currently, there are over 800 MSC-based clinical trials in the United States(ClinicalTrials.gov, 2017).…”

Mesenchymal stem/stromal cells: the therapeutic effects in animal models of acute pulmonary diseases

“…The differentiation power of these cells in vivo and in vitro has led them to be considered as a good tool for regenerative medicine and tissue engineering. With regard to the high proliferation and the ability MSCs differentiation and also because their prominent role in the effects of immune modulation, the ability to migrate to injury sites, the secretion of growth factors, anti-inflammatory cytokines, extracellular vesicles as well as action through differentiation and cell-cell junction [19,20,27,28] can be used as an applied tool for the purpose of cell therapy for the treatment of congenital disorders, degenerative diseases, autoimmune diseases and also as efficient carriers to deliver treatment to specific areas [15,29].…”

Stem Cells and Lung Injury

“…The stem cell therapy is a developing area of intervention to control the disease by treating with specific stem cell markers to an individual. The in vivo experiment on mouse model showed that the administration of human umbilical cordmesenchymal stem cells (UC-MSCs) results in the modulation of inflammatory markers for the survival of the epithelail cell [5]. The mesenchymal stem cells (MSCs) demonstrated the potential therapy in lung diseases [5,6].…”

“…The in vivo experiment on mouse model showed that the administration of human umbilical cordmesenchymal stem cells (UC-MSCs) results in the modulation of inflammatory markers for the survival of the epithelail cell [5]. The mesenchymal stem cells (MSCs) demonstrated the potential therapy in lung diseases [5,6]. These MSCs may collect from the umbilical cord (UC), adipose tissue, bone marrow, skeletal muscle and other tissues.…”