Therapeutic effects of four strains of probiotics on experimental colitis in mice

Chen, Lin Lin; Wang, Xue Hong; Chen, Yi; Lian, Guang Hui; Zhang, Jie; Ouyang, Chun Hui; Lu, Fang

doi:10.3748/wjg.15.321

Cited by 53 publications

(34 citation statements)

References 26 publications

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“…The intestinal anti-inflammatory/immunomodulatory properties of Lactobacillus sp. have been previously described in experimental models of colitis (7,8). Our MPO results are in accordance with previous studies of Chen et al (8) showing Ͼ50% (2-fold) reduction in MPO levels by L. acidophilus in Balb/c mice treated for 5 days with 5% DSS.…”

Section: Discussionsupporting

confidence: 93%

“…have been previously described in experimental models of colitis (7,8). Our MPO results are in accordance with previous studies of Chen et al (8) showing Ͼ50% (2-fold) reduction in MPO levels by L. acidophilus in Balb/c mice treated for 5 days with 5% DSS. The almost complete prevention of increased MPO levels by L. acidophilus observed in L. acidophilus ϩ DSS mice could be due to the lower concentration of DSS (3%) used in our current studies compared with 5% DSS used by Chen et al In addition, loose fecal pellets were observed in the DSS mice, but not in any other group, including the L. acidophilus ϩ DSS mice.…”

Section: Discussionsupporting

confidence: 93%

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Upregulation of P-glycoprotein by probiotics in intestinal epithelial cells and in the dextran sulfate sodium model of colitis in mice

Saksena

Goyal²,

Raheja³

et al. 2011

American Journal of Physiology-Gastrointestinal and Liver Physi

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. Upregulation of P-glycoprotein by probiotics in intestinal epithelial cells and in the dextran sulfate sodium model of colitis in mice. Am J Physiol Gastrointest Liver Physiol 300: G1115-G1123, 2011. First published February 24, 2011 doi:10.1152/ajpgi.00027.2011.-P-glycoprotein (P-gp) mediates efflux of xenobiotics and bacterial toxins from the intestinal mucosa into the lumen. Dysregulation of P-gp has been implicated in inflammatory bowel disease. Certain probiotics have been shown to be effective in treating inflammatory bowel disease. However, direct effects of probiotics on P-gp are not known. Current studies examined the effects of Lactobacilli on P-gp function and expression in intestinal epithelial cells. Caco-2 monolayers and a mouse model of dextran sulfate sodium-induced colitis were utilized. P-gp activity was measured as verapamil-sensitive [ 3 H]digoxin transepithelial flux. Multidrug resistant 1 (MDR1)/P-gp expression was measured by real-time quantitative PCR and immunoblotting. Culture supernatant (CS; 1:10 or 1:50, 24 h) of Lactobacillus acidophilus or Lactobacillus rhamnosus treatment of differentiated Caco-2 monolayers (21 days postplating) increased (ϳ3-fold) MDR1/P-gp mRNA and protein levels. L. acidophilus or L. rhamnosus CS stimulated P-gp activity (ϳ2-fold, P Ͻ 0.05) via phosphoinositide 3-kinase and ERK1/2 MAPK pathways. In mice, L. acidophilus or L. rhamnosus treatment (3 ϫ 10 9 colonyforming units) increased mdr1a/P-gp mRNA and protein expression in the ileum and colon (2-to 3-fold). In the dextran sulfate sodium (DSS)-induced colitis model (3% DSS in drinking water for 7 days), the degree of colitis as judged by histological damage and myeloperoxidase activity was reduced by L. acidophilus. L. acidophilus treatment to DSS-treated mice blocked the reduced expression of mdr1a/ P-gp mRNA and protein in the distal colon. These findings suggest that Lactobacilli or their soluble factors stimulate P-gp expression and function under normal and inflammatory conditions. These data provide insights into a novel mechanism involving P-gp upregulation in beneficial effects of probiotics in intestinal inflammatory disorders. multidrug resistance 1; Lactobacillus species; phosphoinositide 3-kinase; ERK1/2 kinase; dextran sulfate sodium-induced colitis; intestinal inflammation

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Section: Discussionsupporting

confidence: 93%

Section: Discussionsupporting

confidence: 93%

Upregulation of P-glycoprotein by probiotics in intestinal epithelial cells and in the dextran sulfate sodium model of colitis in mice

Saksena

Goyal²,

Raheja³

et al. 2011

American Journal of Physiology-Gastrointestinal and Liver Physi

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“…Probiotic foods containing LAB have been proposed as a natural alternative to improve the general health status, preventing various gastrointestinal disorders such as gastric ulcers and inflammation related to H. pylori infection, gastrointestinal infections (Lebeer et. al., 2010) or antibiotic-associated diarrhea (Chen et. al., 2009;Gill & Guarner, 2004;Penner et.…”

Section: Lactic Acid Bacteria In Gastritismentioning

confidence: 99%

New Approaches in Gastritis Treatment

Marcial¹,

Rodríguez²,

Medici³

et al. 2011

Gastritis and Gastric Cancer - New Insights in Gastroprotection, Diagnosis and Treatments

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“…The results of Chen and colleagues 71 show that in the same model of colitis, different strains of probiotics are capable of achieving similar effects. When the model of colitis is changed however, so does the aetiology of the disease and the effects of probiotics.…”

Section: Therapeutic Effects Of Probiotics In Animalsmentioning

confidence: 97%

“…In addition to their prophylactic study, Mane and colleagues 55 ] were able to reduce, at least in part, the effects of colitis. Effects of DSS-induced colitis included: body mass loss, 70,71 higher disease activity index scores, 71 shortening of the colon, 70 reduced ZO-1 mRNA expression in IECs, 70 increased leukocyte infiltration in the colon, 70 increased Na + absorption in the colonic mucosa, 70 increased MPO activity 71 and colonic IL-1β production, coupled with lower IL-4 production. 71 All strains were effective in reducing the effects of colitis, EcN and E. faecalis proved most effective.…”

Section: Therapeutic Effects Of Probiotics In Animalsmentioning

confidence: 99%

The effects of multi-strain probiotics on liver disease

Briskey¹

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Therapeutic effects of four strains of probiotics on experimental colitis in mice

Abstract: The four strains of probiotics have beneficial effects on experimental colitis in mice. E. faecalis has a better effect on DSS-induced experimental colitis in mice than the other three strains. Supplement of probiotics provides a new therapy for UC.

Cited by 53 publications

References 26 publications

Upregulation of P-glycoprotein by probiotics in intestinal epithelial cells and in the dextran sulfate sodium model of colitis in mice

Upregulation of P-glycoprotein by probiotics in intestinal epithelial cells and in the dextran sulfate sodium model of colitis in mice

New Approaches in Gastritis Treatment

The effects of multi-strain probiotics on liver disease

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