Therapeutic effect of Dongbai-Tonglin-Fang, a Chinese herbal formula, on urinary tract infection in rat model

Li, Xiaoqin; Zhu, Jian-Yong; Pan, Rongrong; Shen, Yu-Li; Rahman, Khalid; Zhang, Lijun; Luan, Xin; Zhang, Hong

doi:10.1016/j.jep.2019.112028

Cited by 6 publications

(2 citation statements)

References 19 publications

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“…Female rats were water fasted for 24 h and randomly divided into five groups of nine rats each: Saline group, UPEC group, 100-Shi (Shionone) group, 200-Shi group, and 100-LVX (levofloxacin hydrochloride, positive control) group. Levofloxacin is a quinolone antibiotic that is relatively effective against the common pathogens of urinary tract infections, such as Gramnegative rods and Gram-positive cocci [14]. After the rats were anesthetized and their perineum disinfected, the F4 ureteral catheter was passed through the urethra for about 3 cm to reach the bladder, the bladder was emptied of residual fluid, and the prepared bacterial solution (300 μL, 1 × 10 7 CFU/each rat) was pushed into the bladder, after which the urethra was clamped with a paper clip for 2-3 h and then released.…”

Section: Animals and Experimentalmentioning

confidence: 99%

Shionone Relieves Urinary Tract Infections by Removing Bacteria from Bladder Epithelial Cells

Yin

Zhu

Jiang

et al. 2023

Cellular Microbiology

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In clinical practice, urinary tract infections (UTIs) are second only to respiratory infections in terms of infectious diseases. In recent years, drug resistance of Escherichia coli (E. coli) has increased significantly. The therapeutic effects of Shionone on UTI were assessed by modelling UTI in SD rats and SV-HUC-1 cells with E. coli solution. After treatment of Shionone, the UTI rat model showed a decrease in wet weight/body weight of bladder, as well as a reduction in cellular inflammatory infiltration of bladder tissue and a decrease in urinary levels of IL-6, IL-1β, and TNF-α. In addition, the levels of proinflammatory factors were significantly reduced in a dose-dependent manner in UTI cell model treated with different doses of Shionone (5, 10, and 20 μg/kg). The results of immunofluorescence analysis in both in vivo and in vitro experiments revealed that Shionone reduced bacterial load and the number of E. coli colonies growing on the plates was greatly reduced. These results suggested that Shionone has a good therapeutic effect on UTI, achieved by reducing bacterial load in bladder epithelial cells. The data presented here provide a basis for further research into the treatment of UTI.

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Section: Animals and Experimentalmentioning

confidence: 99%

Shionone Relieves Urinary Tract Infections by Removing Bacteria from Bladder Epithelial Cells

Yin

Zhu

Jiang

et al. 2023

Cellular Microbiology

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“…AKT1 is a key reg-ulator of host cell survival, inflammatory responses, proliferation, and metabolism ( Wiles et al, 2008 ). It was reported that SRC could inhibit TLR4-induced inflammatory cytokines and promote the anti-inflammatory cytokine IL-10, which plays an important role in the treatment of acute and chronic inflammation ( Li et al, 2019 ). Wittmann et al ( Wittmann et al, 2015 ) found that the increased expression of TLRs in patients might result in counter regulation of the host and serve to prevent intestinal inflammation and disease.…”

Section: Discussionmentioning

confidence: 99%

Chemical profiling of Sanjin tablets and exploration of their effective substances and mechanism in the treatment of urinary tract infections

Wang

et al. 2023

Front. Chem.

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Introduction: Sanjin tablets (SJT) are a well-known Chinese patent drug that have been used to treat urinary tract infections (UTIs) for the last 40 years. The drug consists of five herbs, but only 32 compounds have been identified, which hinders the clarification of its effective substances and mechanism.Methods: The chemical constituents of SJT and their effective substances and functional mechanism involved in the treatment of UTIs were investigated by using high performance liquid chromatography-electrospray ionization-ion trap-time of flight-mass spectrometry (HPLC-ESI-IT-TOF-MSn), network pharmacology, and molecular docking.Results: A total of 196 compounds of SJT (SJT-MS) were identified, and 44 of them were unequivocally identified by comparison with the reference compounds. Among 196 compounds, 13 were potential new compounds and 183 were known compounds. Among the 183 known compounds, 169 were newly discovered constituents of SJT, and 93 compounds were not reported in the five constituent herbs. Through the network pharmacology method, 119 targets related to UTIs of 183 known compounds were predicted, and 20 core targets were screened out. Based on the “compound–target” relationship analysis, 94 compounds were found to act on the 20 core targets and were therefore regarded as potential effective compounds. According to the literature, 27 of the 183 known compounds were found to possess antimicrobial and anti-inflammatory activities and were verified as effective substances, of which 20 were first discovered in SJT. Twelve of the 27 effective substances overlapped with the 94 potential effective compounds and were determined as key effective substances of SJT. The molecular docking results showed that the 12 key effective substances and 10 selected targets of the core targets have good affinity for each other.Discussion: These results provide a solid foundation for understanding the effective substances and mechanism of SJT.

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Discovering inhibitor molecules for pathological crystallization of CaOx kidney stones from natural extracts of medical herbs

Macaringue

Zhou

et al. 2022

Journal of Ethnopharmacology

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Therapeutic effect of Dongbai-Tonglin-Fang, a Chinese herbal formula, on urinary tract infection in rat model

Cited by 6 publications

References 19 publications

Shionone Relieves Urinary Tract Infections by Removing Bacteria from Bladder Epithelial Cells

Shionone Relieves Urinary Tract Infections by Removing Bacteria from Bladder Epithelial Cells

Chemical profiling of Sanjin tablets and exploration of their effective substances and mechanism in the treatment of urinary tract infections

Discovering inhibitor molecules for pathological crystallization of CaOx kidney stones from natural extracts of medical herbs

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