Therapeutic effect of co-transplantation of neuregulin 1-transfected-Schwann cells and bone marrow stromal cells on spinal cord hemisection syndrome

Zhang, Ji Fei; Zhao, Fu Sheng; Wu, Geng; Kong, Qing Fei; Sun, Bo; Cao, Jingyan; Zhang, Yao; Wang, Jing-Hua; Zhang, Jing; Xu, Jin; Li, Hu Lun

doi:10.1016/j.neulet.2011.04.045

Cited by 9 publications

(10 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Previously, our study revealed that transplantation of Schwann cells (SCs) overexpressing NRG1 could de nitely alleviate histological damage after SCI [24]. Moreover, we discovered that cotransplantation of NRG1 overexpressing SCs and BMSCs could reduce spinal cord cystic cavities, and improve motor functional recovery in rats after SCI [45]. In this study, we found that transplantation of NRG1-BMSCs notably reduced neuronal chromatolysis after SCI.…”

Section: Discussionmentioning

confidence: 57%

Transplantation of Neuregulin1-Overexpressing Bone Marrow Mesenchymal Cells Accelerates Motor Functional Recovery by Facilitating Neurite Outgrowth and Reducing Cell Apoptosis in Rat after Spinal Cord Injury

Wu¹,

Liu²,

Zhu³

et al. 2020

Preprint

Self Cite

View full text Add to dashboard Cite

Background: Bone marrow mesenchymal stem cells (BMSCs) transplantation offers an attractive strategy for treating multiply neurological diseases. Neuregulin1 (NRG1) plays fundamental roles in nervous system development and nerve repair. In this study, we aimed to investigate whether transplantation of NRG1-overexpressing BMSCs could alleviate spinal cord injury (SCI), and to explore the possible underling mechanisms. Methods: In vitro, NRG1-overexpressing BMSCs were constructed via plasmid transfection, and co-cultured with PC12 cells subjected to oxygen-glucose deprivation (OGD). Neurite outgrowth, cell viability and apoptosis of PC12 cells were evaluated. In vivo, BMSCs, empty-vector BMSCs and NRG1-overexpressing BMSCs were transplanted respectively into rats with SCI. Rat locomotor functions, neuronal chromatolysis, neurite outgrowth and cell apoptosis were assessed respectively. Results: The results showed that NRG1-overexpressing BMSCs in vitro significantly expedited neurite growth, elevated growth-associated protein 43 expression, enhanced cell viability and rescued ODG-induced apoptosis in PC12 cells. In vivo, transplantation of NRG1-overexpressing BMSCs notably accelerated rat motor functional recovery, attenuated neuronal chromatolysis, promoted neurite outgrowth and reduced cell apoptosis after SCI. Moreover, NRG1-overexpressing BMSCs were also able to regulate apoptosis-related proteins expression after SCI. Conclusions: These findings demonstrate that NRG1-overexpressing BMSCs can accelerate motor functional recovery by facilitating neurite outgrowth and reducing cell apoptosis after SCI, suggesting that NRG1-overexpressing BMSCs may be a promising candidate for the treatment of SCI.

show abstract

Section: Discussionmentioning

confidence: 57%

Transplantation of Neuregulin1-Overexpressing Bone Marrow Mesenchymal Cells Accelerates Motor Functional Recovery by Facilitating Neurite Outgrowth and Reducing Cell Apoptosis in Rat after Spinal Cord Injury

Wu¹,

Liu²,

Zhu³

et al. 2020

Preprint

Self Cite

View full text Add to dashboard Cite

show abstract

“…However, more research is needed in adult animals to determine differences between age, size, and an animal’s ability to regenerate. Previous studies noted the pro‐survival effect of the Nrg1 pathway on nervous cells during neurodegenerative diseases and SCI (Zhang et al , ; ; Modol‐Caballero et al , ) as well as its effect on remyelination (Taveggia et al , ; Fricker et al , ) and stem cell proliferation and differentiation (Ghashghaei et al , ; Sato et al , ; Reeve et al , ; Zhao et al , ). This well‐documented role of Nrg1 in pro‐regenerative processes in the nervous system led us to suspect that it could contribute to spinal cord regeneration in the highly regenerative axolotl salamander.…”

Section: Discussionmentioning

confidence: 95%

“…Additionally, Nrg1/Erbb2 plays a role in neuronal signaling, including axonal growth and guidance (Bermingham‐McDonogh et al , ; Lopez‐Bendito et al , ; Hancock et al , ), neural stem cell proliferation (Liu et al , ; Sato et al , ), and neural migration (Rio et al , ). Furthermore, SCI in mammals leads to an acute and sustained decrease in Nrg1, and supplementation of Nrg1 after injury can induce neuroprotection (Alizadeh et al , ; ), increase neural progenitor cell proliferation into oligodendrocytes (Whittaker et al , ; Gauthier et al , ), and promote axonal regeneration (Zhang et al , ) and remyelination of naked axons (Bartus et al , ; ). Moreover, Nrg1 is required for multiple regeneration paradigms, including axolotl limb regeneration (Farkas et al , ), axolotl lung regeneration (Jensen et al , ), and heart regeneration in zebrafish (Gemberling et al , ) and mice (Mahmoud et al , ).…”

Section: Introductionmentioning

confidence: 99%

Investigating Nrg1 Signaling in the Regenerating Axolotl Spinal Cord Using Multiplexed FISH

Freitas

Lovely

Monaghan

2019

Developmental Neurobiology

View full text Add to dashboard Cite

Amputation of a salamander tail leadsto functional spinal cord regeneration through activation of endogenous stem cells. Identifying the signaling pathways that control cell proliferation in these neural stem cells will help elucidate the mechanisms underlying the salamander's regenerative ability. Here, we show that neuregulin 1 (Nrg1)/ErbB2 signaling is an important pathway in the regulation of neural stem cell proliferation in the spinal cord of the axolotl salamander (Ambystoma mexicanum ). Simultaneous localization of nrg1 mRNA and Nrg1 protein was performed by utilizing a hybridization chain reaction fluorescence in situ hybridization (FISH) methodology in tissue sections. Multiplexed FISH also permitted the phenotyping of multiple cell types on a single fixed section allowing the characterization of mRNA expression, protein expression, and tissue architecture. Pharmacological inhibition of ErbB2 showed that intact Nrg1/ErbB2 signaling is critical for adult homeostatic regeneration as well as for injury-induced spinal cord regeneration. Overall, our results highlight the importance of the NRG1/ErbB2 signaling pathway in neural stem cell proliferation in the axolotl.

show abstract

“…The BBB results here presented for injured animals without treatment are in concordance with other previously published experiments. 20,21 However, others also report a greater motor recovery from control animals (between 6 and 8 on the . The laminectomy alone did not affect the spinal cord of sham animals (c).…”

Section: Discussionmentioning

confidence: 99%

Benefits of Spine Stabilization with Biodegradable Scaffolds in Spinal Cord Injured Rats

Silva

Sousa

Fraga

et al. 2013

Tissue Engineering Part C: Methods

View full text Add to dashboard Cite

Therapeutic effect of co-transplantation of neuregulin 1-transfected-Schwann cells and bone marrow stromal cells on spinal cord hemisection syndrome

Cited by 9 publications

References 13 publications

Transplantation of Neuregulin1-Overexpressing Bone Marrow Mesenchymal Cells Accelerates Motor Functional Recovery by Facilitating Neurite Outgrowth and Reducing Cell Apoptosis in Rat after Spinal Cord Injury

Transplantation of Neuregulin1-Overexpressing Bone Marrow Mesenchymal Cells Accelerates Motor Functional Recovery by Facilitating Neurite Outgrowth and Reducing Cell Apoptosis in Rat after Spinal Cord Injury

Investigating Nrg1 Signaling in the Regenerating Axolotl Spinal Cord Using Multiplexed FISH

Benefits of Spine Stabilization with Biodegradable Scaffolds in Spinal Cord Injured Rats

Contact Info

Product

Resources

About