Therapeutic effect of chitosan on CCl4‑induced hepatic fibrosis in rats

Wang, Zhong‐Feng; Wang, Mao‐Yu; Yu, Dehai; Zhao, Yan; Xu, Hongmei; Zhong, Sheng; Sun, Weili; He, Yuhang; Niu, Junqi; Gao, Pujun; Li, Haijun

doi:10.3892/mmr.2018.9343

Cited by 18 publications

(19 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The histomicrographs of Group II demonstrated vivid features of acute toxicity in the form of inflammation and extensive tissue damage ( Figure 9 ). The sinusoids were abruptly disturbed, showing that tissue fibrosis and nuclear damage were in most of the hepatocytes, as previously documented [ 51 , 54 ]. The sections from this group demonstrated typical liver injury evidenced by noticeable macrosteatosis, ballooning of several hepatocytes, loss of cytoplasm, and pushing of nuclei towards one side, depicting the cellular necrosis.…”

Section: Resultssupporting

confidence: 70%

Bioactive Tryptophan-Based Copper Complex with Auxiliary β-Carboline Spectacle Potential on Human Breast Cancer Cells: In Vitro and In Vivo Studies

et al. 2021

View full text Add to dashboard Cite

Biocompatible tryptophan-derived copper (1) and zinc (2) complexes with norharmane (β-carboline) were designed, synthesized, characterized, and evaluated for the potential anticancer activity in vitro and in vivo. The in vitro cytotoxicity of both complexes 1 and 2 were assessed against two cancerous cells: (human breast cancer) MCF7 and (liver hepatocellular cancer) HepG2 cells with a non-tumorigenic: (human embryonic kidney) HEK293 cells. The results exhibited a potentially decent selectivity of 1 against MCF7 cells with an IC50 value of 7.8 ± 0.4 μM compared to 2 (less active, IC50 ~ 20 μM). Furthermore, we analyzed the level of glutathione, lipid peroxidation, and visualized ROS generation to get an insight into the mechanistic pathway and witnessed oxidative stress. These in vitro results were ascertained by in vivo experiments, which also supported the free radical-mediated oxidative stress. The comet assay confirmed the oxidative stress that leads to DNA damage. The histopathology of the liver also ascertained the low toxicity of 1.

show abstract

Section: Resultssupporting

confidence: 70%

Bioactive Tryptophan-Based Copper Complex with Auxiliary β-Carboline Spectacle Potential on Human Breast Cancer Cells: In Vitro and In Vivo Studies

et al. 2021

View full text Add to dashboard Cite

show abstract

“…Several chitosan‐based biomaterials have been applied with promising results as drug, cell, and gene delivery vehicles 13 . Wang et al 14 reported that chitosan treatment significantly improved CCl 4 ‐induced hepatic fibrosis, by decreasing the serum levels of AST, ALT, and ALP; improving liver histology, and decreasing the expression levels of collagen type 3 (COL) 3 and alpha‐smooth muscle actin (α‐SMA) and was recommended as an alternative approach for the clinical treatment of hepatic fibrosis. Pretreatment with chitosan nanoparticles exhibited a significant reduction in cadmium chloride‐induced gastric mucosal hemorrhagic injury in a dose‐dependent manner 15 .…”

Section: Introductionmentioning

confidence: 99%

Chitosan attenuated the neurotoxicity‐induced titanium dioxide nanoparticles in brain of adult rats

Halawa

Elshopakey

El‐Adl

et al. 2021

Environmental Toxicology

View full text Add to dashboard Cite

In the current study, we aimed to investigate the neurotoxic effect of oral titanium dioxide nanoparticles (TiO2 NPs) as well as the possible neuroprotective effect of carboxymethyl chitosan in adult rats for 14 days. The results revealed that TiO2 NPs inhibited the activity of the acetylcholine esterase enzyme and the levels of serotonin, dopamine, and norepinephrine neurotransmitters. Additionally, it induced neuro‐oxidative stress and neuroinflammation via an elevation in MDA levels and IL‐6, while GSH concentration, as well as GPx and GST activities, were decreased. TiO2 NPs induced neuronal apoptosis through upregulation of the expression of caspase‐8 and ‐9 that was further confirmed by increasing caspases‐3 and ‐8 proteins in the hippocampus, cerebral cortex, and cerebellum. The expression of the immediate‐early gene BDNF was increased in response to TiO2 NPs, while that of Arc was reduced. Chitosan significantly attenuated the TiO2 NPs‐induced neurotoxicity regarding AChE, serotonin, MDA, GSH, GPx, GST, IL‐6, caspases‐8, ‐9, and ‐3. Chitosan inhibited the expression of Arc and alleviated the effect of TiO2 NPs on BDNF expression. Collectively, TiO2 NPs induced neurotoxicity via their action on vital neuronal biomarkers that might in turn cause brain dysfunction. Despite the neuroprotection of chitosan, its inhibitory effect on Arc expression should be considered.

show abstract

“…This also can explain the observed marked inhibitions in the serum activities of ASAT, ALAT, ALP, and CK, with increasing the dose of chitosan in HFD. These findings could be ascribed to the antioxidant activities of chitosan (Wang et al, 2018) and vitamin E (Rizvi et al, 2014) that improved protection of liver and heart against the adverse effects of fat accumulation. This can be also attributed to the above mentioned ability of chitosan to eliminate fats.…”

Section: Discussionmentioning

confidence: 99%

Ameliorative role of a combination of chitosan, calcium, vitamins A and E against high fat diets-induced adverse effects in rats

Ali¹,

Mansour²,

Hassanine³

et al. 2019

JoBAZ

View full text Add to dashboard Cite

Background: Chitosan is a promising natural source for controlling obesity. Obese people tend to use supplementary nutrients to compensate for their deficiencies in their diets. The present study aimed to investigate the combined effect of chitosan, calcium, and vitamins A and E supplements against the adverse effects of high-fat diet consumption in rats. Methods: Twenty-five male albino rats were assigned into five equal groups. Control rats were fed standard basal diet (SBD). Another group was fed high-fat diet (HFD) without supplements. The other three groups were fed HFD containing 0, 400, and 800 mg of chitosan per kilogram diet, in presence of calcium and vitamins A and E supplements, daily for 10 weeks. Results: As compared to controls, rats fed HFD without supplements showed significant elevations in body weight gain, feed consumption, relative weights of the heart and liver, serum levels of total cholesterol, triglycerides, low-density lipoprotein cholesterols, very low-lipoprotein triglycerides, urea, creatinine, and malondialdehyde, as well as serum activities of alanine and aspartate aminotransferases, alkaline phosphatase, and creatine kinase. Moreover, significant declines in the relative kidney weight, serum levels of high-density lipoprotein cholesterols, total proteins, albumin, globulin, calcium, vitamins A and E, erythrocyte glutathione content, and superoxide dismutase activity were recorded. Histopathological alterations were observed in rats fed HFD with or without supplements. On contrary, rats fed HFD containing chitosan and supplements showed remarkable improvement in all the studied parameters towards control values, in dose-dependent manner. Conclusion: Chitosan, calcium, and vitamins A and E attenuated the adverse effects caused by HFD intake in rats.

show abstract

Therapeutic effect of chitosan on CCl4‑induced hepatic fibrosis in rats

Cited by 18 publications

References 29 publications

Bioactive Tryptophan-Based Copper Complex with Auxiliary β-Carboline Spectacle Potential on Human Breast Cancer Cells: In Vitro and In Vivo Studies

Bioactive Tryptophan-Based Copper Complex with Auxiliary β-Carboline Spectacle Potential on Human Breast Cancer Cells: In Vitro and In Vivo Studies

Chitosan attenuated the neurotoxicity‐induced titanium dioxide nanoparticles in brain of adult rats

Ameliorative role of a combination of chitosan, calcium, vitamins A and E against high fat diets-induced adverse effects in rats

Contact Info

Product

Resources

About