Bioactive Tryptophan-Based Copper Complex with Auxiliary β-Carboline Spectacle Potential on Human Breast Cancer Cells: In Vitro and In Vivo Studies

Alharbi, Walaa; Hassan, Iftekhar; Khan, Rais Ahmad; Parveen, Shazia; Alharbi, Khadijah H.; BinSharfan, Ibtisam I.; Alhazza, Ibrahim M.; Ebaid, Hossam; Alsalme, Ali

doi:10.3390/molecules26061606

Cited by 6 publications

(1 citation statement)

References 80 publications

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“…Nevertheless, MCF-7 cells treated with complex two produced a non-significant increase in MDA levels compared with untreated cells. MDA levels increased in MCF-7 cells treated with complex three (copper complex) as compared to untreated cells; similar results were reported by Khan et al [ 125 ] and Alharbi et al [ 126 ]. The highest MDA levels were seen after treatment with the copper complex.…”

Section: Resultssupporting

confidence: 87%

Study of novel bidentate heterocyclic amine-based metal complexes and their biological activities: cytotoxicity and antimicrobial activity evaluation

et al. 2023

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Metallic antitumor drugs with heterocyclic ligands, such as novel AMI (amino methyl imidazole) complexes [Pd(AMI)Cl2](1), [Cu(AMI)L1](2), and [Cu(AMI)L2·2H2O](3) where L1 = oxalate and L2 = malonate, were synthesized and characterized. Assessments included elemental analyses, mass spectrometry, Fourier transform-infrared spectroscopy, ultraviolet–visible spectroscopy, and thermal analysis. The cytotoxicity of AMI complexes compared to cisplatin was assessed using MTT (3-[4,5-dimethylthiazol-2-yl] 2,5diphenyl tetrazolium bromide) assay with breast (MCF-7) and cervical (HeLa) cancer cell lines. After treating these cells with the AMI complexes' IC50 values for 48 h, malondialdehyde levels and catalase activity were used to assess oxidative stress, antioxidant activity was evaluated with DPPH radical scavenging method, comet assays assessed DNA damage, and DNA fragmentation was evaluated using the gel electrophoresis. In vitro, antimicrobial activity was assessed using a disc diffusion method. The anticancer activity results showed that IC50 (half-maximal inhibitory concentration) values of complex one, two, and three against MCF-7 and HeLa cancer cells are 0.156 ± 0.0006, 0.125 ± 0.001, 0.277 ± 0.002 μM respectively for MCF-7 cells and 0.222 ± 0.0005, 0.126 ± 0.0009, 0.152 ± 0.001 μM respectively for HeLa cells. Complex two demonstrated strong anticancer activity against MCF-7 and Hela cells. The study of oxidative stress parameters revealed that Malondialdehyde levels increased in cancer cell lines treated with complexes compared to untreated cells. Catalase activity decreased in cells treated with palladium chelate. The DPPH radical scavenging assay results identified that complex one was a more potent antioxidant in MCF-7 and Hela cells than other complexes with SC50 values of 227.5 ± 0.28 and 361 ± 1.2 μL/mL, respectively. The comet assay results showed that complex two caused significant DNA damage in MCF-7 and HeLa cancer cells treated. Antimicrobial assays identified complex three as the most effective. Copper complexes give better antifungal activity against A. flavus than the palladium complex. We conclude that complex two is the most active in both cell types and might be assessed as a clinically useful drug for breast cancer treatment. The significance of the current study is the synthesis of antitumor drugs containing heterocyclic ligands, such as novel AMI complexes, and the study of their biological activities.

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Section: Resultssupporting

confidence: 87%

Study of novel bidentate heterocyclic amine-based metal complexes and their biological activities: cytotoxicity and antimicrobial activity evaluation

et al. 2023

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Anticancer Potential of β‐Carboline Alkaloids: An Updated Mechanistic Overview

Tshikhudo,

Mabhaudhi,

Koorbanally

et al. 2024

Chemistry & Biodiversity

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This comprehensive review is designed to evaluate the anticancer properties of β‐carbolines derived from medicinal plants, with the ultimate goal of assessing their suitability and potential in cancer treatment, management, and prevention. An exhaustive literature survey was conducted on a wide array of β‐carbolines including, but not limited to, harmaline, harmine, harmicine, harman, harmol, harmalol, pinoline, tetrahydroharmine, tryptoline, cordysinin C, cordysinin D, norharmane, and perlolyrine. Various analytical techniques were employed to identify and screen these compounds, followed by a detailed analysis of their anticancer mechanisms. Natural β‐carbolines such as harmaline and harmine have shown promising inhibitory effects on the growth of cancer cells, as evidenced by multiple in vitro and in vivo studies. Synthetically derived β‐carbolines also displayed noteworthy anticancer, neuroprotective, and cognitive‐enhancing effects. The current body of research emphasizes the potential of β‐carbolines as a unique source of bioactive compounds for cancer treatment. The diverse range of β‐carbolines derived from medicinal plants can offer valuable insights into the development of new therapeutic strategies for cancer management and prevention.

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A review on metal complexes and its anti-cancer activities: Recent updates from in vivo studies

Adhikari,

Nath,

Das

et al. 2024

Biomedicine & Pharmacotherapy

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Bioactive Tryptophan-Based Copper Complex with Auxiliary β-Carboline Spectacle Potential on Human Breast Cancer Cells: In Vitro and In Vivo Studies

Cited by 6 publications

References 80 publications

Study of novel bidentate heterocyclic amine-based metal complexes and their biological activities: cytotoxicity and antimicrobial activity evaluation

Study of novel bidentate heterocyclic amine-based metal complexes and their biological activities: cytotoxicity and antimicrobial activity evaluation

Anticancer Potential of β‐Carboline Alkaloids: An Updated Mechanistic Overview

A review on metal complexes and its anti-cancer activities: Recent updates from in vivo studies

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