Therapeutic Effect Analysis of Sinomenine on Rat Cerebral Ischemia–Reperfusion Injury

Shen, Yang; Ning, Fangbo; Li, Juan; Guo, Dongmei; Zhang, Li; Cui, Ruiting; Liu, Yun-lin

doi:10.1016/j.jstrokecerebrovasdis.2016.02.023

Cited by 28 publications

(24 citation statements)

References 26 publications

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“…The findings of biochemical assessment showed that a cerebral I/R injury could lead to an increase in plasma LDH level, consistent with the previous study [20]. LDH, an indirect marker of hypoxia, was measured to evaluate cerebral ischemia damage.…”

Section: Discussionsupporting

confidence: 88%

A NMR-Based Metabonomics Approach to Determine Protective Effect of a Combination of Multiple Components Derived from Naodesheng on Ischemic Stroke Rats

Luo

Kang

et al. 2019

Molecules

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Naodesheng (NDS) is a widely used traditional Chinese medicine (TCM) prescription for the treatment of ischemic stroke. A combination of 10 components is derived from NDS. They are: Notoginsenoside R1, ginsenoside Rg1, ginsenoside b1, ginsenoside Rd, hydroxysafflor yellow A, senkyunolide I, puerarin, daidzein, vitexin, and ferulic acid. This study aimed to investigate the protective effect of the ten-component combination derived from NDS (TCNDS) on ischemic stroke rats with a middle cerebral artery occlusion (MCAO) model by integrating an NMR-based metabonomics approach with biochemical assessment. Our results showed that TCNDS could improve neurobehavioral function, decrease the cerebral infarct area, and ameliorate pathological features in MCAO model rats. In addition, TCNDS was found to decrease plasma lactate dehydrogenase (LDH) and malondialdehyde (MDA) production and increase plasma superoxide dismutase (SOD) production. Furthermore, 1H-NMR metabonomic analysis indicated that TCNDS could regulate the disturbed metabolites in the plasma, urine, and brain tissue of MCAO rats, and the possible mechanisms were involved oxidative stress, energy metabolism, lipid metabolism, amino acid metabolism, and inflammation. Correlation analysis were then performed to further confirm the metabolites involved in oxidative stress. Correlation analysis showed that six plasma metabolites had high correlations with plasma LDH, MDA, and SOD. This study provides evidence that an NMR-based metabonomics approach integrated with biochemical assessment can help to better understand the underlying mechanisms as well as the holistic effect of multiple compounds from TCM.

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Section: Discussionsupporting

confidence: 88%

A NMR-Based Metabonomics Approach to Determine Protective Effect of a Combination of Multiple Components Derived from Naodesheng on Ischemic Stroke Rats

Luo

Kang

et al. 2019

Molecules

View full text Add to dashboard Cite

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“…Mechanisms of cerebral ischemia-reperfusion injury are complex. In response to these mechanisms, traditional Chinese medicine and its effective ingredients have achieved some research progress on the prevention and treatment of cerebral ischemia-reperfusion injury (Zhang et al, 2016, Yang et al, 2016). At present, the research on drugs to prevent and treat cerebral ischemia focuses on studying the active sites or extracts of traditional Chinese medicine, and the research on compound prescription is mere research on single traditional Chinese medicine (Xue et al, 2016, Duan et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

The protective role of verbenalin in rat model of focal cerebral ischemia reperfusion

Cao

Miao

Qiao

et al. 2018

Saudi Journal of Biological Sciences

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To investigate the protective mechanism of verbenalin on cerebral ischemia-reperfusion injury. Middle cerebral artery occlusion in the left hemisphere was induced in rats by filament insertion, and rat model of focal cerebral ischemia-reperfusion was established. The high, medium and low dose of verbenalin groups were injected in the tail vein of corresponding drugs 10 min before reperfusion, and submitted for 22 h of reperfusion after the operation. Mortality rate was then calculated, and neurological deficits of rats were scored. The serum of rats was got to determine the S-100β protein level, and the brain tissue was removed to determine the levels of Bax, Bcl-2, Caspase-3 and ATPase. TTC staining was performed on the brain tissue to calculate the percentage of cerebral infarct size. Changes in brain tissue morphology were observed. Rat model of focal cerebral ischemia-reperfusion was successfully replicated. In groups that have taken different doses of verbenalin, the mortality rate, neurological deficit score and the percentage of cerebral infarction size were significantly reduced, and the levels of Bax, Caspase-3, S-100β level of the serum in the brain tissue were also significantly reduced. Increases in the levels of Bcl-2 and ATPase in brain tissue and improvement of pathological damage of hippocampus and cortex were observed. Verbenalin can inhibit the expression of apoptosis genes, promote the expression of anti-apoptosis genes, improve brain microcirculation and energy metabolism, hence reducing cerebral ischemia-reperfusion injury.

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“…The mechanisms of cerebral ischemia reperfusion injury are complex, in light of these mechanisms, the effective ingredients of Chinese traditional medicine and single Chinese traditional medicine have made certain achievements for prevention and treatment of cerebral ischemia reperfusion injury (Zhang et al, 2016, Yang et al, 2016,). At present, most of researches on the prevention and treatment of cerebral ischemia are oriented in the active parts or extraction of traditional Chinese medicine, and there are more researches on compound prescription than on single Chinese traditional medicine (Xue et al, 2016, Duan et al, 2017) In particular, there is lack of research on single active ingredient, resulting in obscure mechanism for prevention and treatment of cerebral ischemia reperfusion injury by traditional Chinese medicine and low pertinency.…”

Section: Discussionmentioning

confidence: 99%

Protective effect of chlorogenic acid on the focal cerebral ischemia reperfusion rat models

Miao

Cao

et al. 2017

Saudi Pharmaceutical Journal

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Therapeutic Effect Analysis of Sinomenine on Rat Cerebral Ischemia–Reperfusion Injury

Cited by 28 publications

References 26 publications

A NMR-Based Metabonomics Approach to Determine Protective Effect of a Combination of Multiple Components Derived from Naodesheng on Ischemic Stroke Rats

A NMR-Based Metabonomics Approach to Determine Protective Effect of a Combination of Multiple Components Derived from Naodesheng on Ischemic Stroke Rats

The protective role of verbenalin in rat model of focal cerebral ischemia reperfusion

Protective effect of chlorogenic acid on the focal cerebral ischemia reperfusion rat models

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