Therapeutic drug monitoring of topiramate by liquid chromatography-tandem mass spectrometry

Multiplexed quantification of 63 proteins in human urine by multiple reaction monitoring-based mass spectrometry for discovery of potential bladder cancer biomarkers

“…In this technique, specific transitions from precursor peptides to fragment product ions, produced under collisioninduced dissociation, are selected. MRM-MS has been widely used as a "gold standard" approach for pharmaceuticals as well as for other low-molecular-weight compounds in a variety of fields [22][23][24][25][26]. Given the worldwide focus on biomarker discovery, MRM-MS is now being used as an effective tool for accurate quantitation of candidate proteins.…”

Therapeutic drug monitoring and LC–MS/MS

“…Topiramate can also be ionised in positive ion mode, but sensitivity is increased if negative ionisation is used [77][78][79][80][81][82]. A C18 analytical column is commonly used for quantification of these drugs.…”

Simple and sensitive high performance liquid chromatography method with fluorescence detection for therapeutic drug monitoring of topiramate

“…For various reasons, including insufficient analytical sensitivity 12,23,24,26,28,29,31,35 and linearity, 24,25,[32][33][34]36 previously reported liquid chromatography techniques coupled with various detection systems can not accurately determine full therapeutic range of TPM concentrations. These methods either lack adequate sensitivity, and consequently cannot be applied in pharmacokinetic studies, or the method range is to narrow and therefore they are not practical for routine TDM.…”

“…1,10,11 Drug interactions and individual factors such as age and renal function can markedly affect TPM concentra-Milosheska et al : Simple and Sensitive High Performance Liquid ... tion, suggesting that there is some room for improvement in its clinical use. 6,12 Therapeutic drug monitoring (TDM) may be useful in dose optimization on the initially prescribed treatment, in ascertaining drug compliance, in attributing toxicity to drug treatment, and in managing overdoses and drug-drug interactions. Nevertheless, the value of TDM of TPM has not been established due to lack of documented correlation between serum concentration and drug effects.…”

“…15 Various analytical methods have been reported for measurement of TPM in biological fluids, including immunoassays, 16,17 gas chromatography (GC) coupled to flame ionization detection (FID) 18 or nitrogen phosphorous detection (NPD), [19][20][21][22] high performance liquid chromatography with UV (HPLC-UV) 23 or fluorescence detection (HPLC-FLD), [24][25][26] capillary electrophoresis with UV detection, 27 liquid chromatography coupled to mass spectrometry (LC-MS), 28,29 and more recently, liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS). 12,[30][31][32][33][34][35][36] The analysis of TPM in biological fluids is complicated, because like most other carbohydrates and their derivatives, TPM does not contain any chromophores that absorb above 190 nm. Consequently, TPM has extremely low UV-visible absorbance and no spontaneous fluorescence, and cannot be directly analyzed by conventional HPLC with UV or fluorescence detection.…”