Therapeutic Delivery of miR-143 Targeting Tumor Metabolism in Poorly Differentiated Thyroid Cancer Xenografts and Efficacy Evaluation Using <sup>18</sup>F-FDG MicroPET-CT

Miao, Ying; Zhang, Ling-Fei; Zhang, Min; Guo, Rui; Lin, Li; Li, Biao

doi:10.1089/hum.2018.160

Cited by 11 publications

(7 citation statements)

References 36 publications

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“…Looking into energy metabolic regulations, we proceeded to investigate glycometabolism. The results of glucose tolerance, pyruvate tolerance, and glucose uptake experiments showed that betaine significantly promoted glucose utilization and inhibited gluconeogenesis, which were consistent with previous studies. ,, Also consistent with previous studies, − glucose utilization was promoted by miR-143 knockout. Furthermore, we are the first to report that miR-143 knockout significantly inhibited gluconeogenesis.…”

Section: Discussionsupporting

confidence: 91%

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miR-143-Mediated Responses to Betaine Supplement Repress Lipogenesis and Hepatic Gluconeogenesis by Targeting MAT1a and MAPK11

Chen

Luo

Yang

et al. 2022

J. Agric. Food Chem.

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The liver as the central organ is responsible for lipogenesis, gluconeogenesis and one-carbon metabolism. Methyl donors (e.g., betaine) modulate metabolic homeostasis and gene regulation through one-carbon metabolism. MiR-143 regulates DNA methylation by targeting DNMT3A, thereby suggesting that this miRNA participates in one-carbon metabolic pathways. However, the effect and mechanism that regulate glucose and lipid metabolism via the methyl group metabolism pathway remain elusive. In this study, we found that a betaine supplement and miR-143 KO significantly promoted lipolysis and glucose utilization and repressed lipogenesis and gluconeogenesis through enhancing energy consumption and thermogenesis, repressing GPNMB and targeting MAPK11, respectively. We further explored the relationship between miR-143 and a methyl donor (betaine) and the miR-143-mediated responses to the betaine supplement regulating the mechanism of the glucose and lipid metabolism. The results showed that betaine significantly down-regulated the expression of miR-143 that subsequently increased SAM levels in the liver by targeting MAT1a. In brief, the regulations of glucose and lipid metabolism are related to the miR-143-regulation of one-carbon units, and the relationship between betaine and miR-143 in the methionine cycle is a typical yin−yang type of regulation. Thus, betaine and miR-143 function together as key regulators and biomarkers for preventing and diagnosing metabolic diseases such as fatty liver disease, obesity, and diabetes.

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Section: Discussionsupporting

confidence: 91%

“…Recently, studies have shown that miR-143 is extensively involved in the regulation of lipid and glucose metabolism . It can promote lipid synthesis, − impair glycolysis and reduce utilization of glucose, − and decrease insulin sensitivity. − …”

Section: Introductionmentioning

confidence: 99%

miR-143-Mediated Responses to Betaine Supplement Repress Lipogenesis and Hepatic Gluconeogenesis by Targeting MAT1a and MAPK11

Chen

Luo

Yang

et al. 2022

J. Agric. Food Chem.

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“…Studies in vitro and in vivo demonstrated that miR-143 directly targets Hexokinase 2 (HK2) and thereby down-regulates its expression. miR-143-mediated HK2 downregulation resulted in suppressed glycolysis and therefore, decreased proliferation and migration (90). These support the tumor-suppressive role of miR-143; however, the expression profile of this miRNA remained to be investigated in clinical thyroid cancer samples.…”

Section: Glucose Metabolism-mediated Tumorigenic Effects Of Dysregulamentioning

confidence: 83%

“…The higher activity of glycolytic pathway has been shown in thyroid cancer to impose tumorigenesis (88,89). Of note, miR-143 and miR-125a-5p have been reported to affect thyroid tumorigenesis via regulating glucose metabolism (90,91). Studies in vitro and in vivo demonstrated that miR-143 directly targets Hexokinase 2 (HK2) and thereby down-regulates its expression.…”

Section: Glucose Metabolism-mediated Tumorigenic Effects Of Dysregulamentioning

confidence: 99%

Non-Coding RNAs: Uncharted Mediators of Thyroid Cancer Pathogenesis

Tabatabaeian¹,

Yang²,

Tay³

2020

Preprint

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Thyroid cancer is the most prevalent malignancy of the endocrine system and the ninth most common cancer globally. Despite the advances in the management of thyroid cancer, there are critical issues with the diagnosis and treatment of thyroid cancer that result in the poor overall survival of undifferentiated and metastatic thyroid cancer patients. Recent studies have revealed the role of different non-coding RNAs (ncRNAs), such as microRNAs (miRNAs), long non-coding RNAs (lncRNAs) and circular RNAs (circRNAs) that are dysregulated during thyroid cancer development or the acquisition of resistance to therapeutics, and may play key roles in treatment failure and poor prognosis of the thyroid cancer patients. Here, we systematically review the emerging roles and molecular mechanisms of ncRNAs that regulate thyroid tumorigenesis and drug response. We then propose the potential clinical implications of ncRNAs as novel diagnostic and prognostic biomarkers for thyroid cancer.

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“…The results showed that the uptake of 18F-FDG in tumors was reduced, which corresponded to the downregulation of Hexokinase 2 (HK2) expression in tissues. Results suggested that miR-143 could be used to specifically assess the therapeutic efficacy of advanced thyroid cancer xenografts by 18F-FDG-microPET/CT [ 18 ]. Therefore, we considered whether the expression of miRNA-216b in NSCLC patients was related to the uptake of 18F-FDG.…”

Section: Introductionmentioning

confidence: 99%

The expression of miRNA-216b is negatively correlated with 18F-FDG uptake in non-small cell lung cancer

et al. 2021

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Background This study aimed to investigate the correlation between miRNA-216b expression in patients with non-small cell lung cancer (NSCLC) and 18F-fluorodeoxyglucose (FDG) uptake by PET/CT and to explore the clinical application value of 18F-FDG PET/CT in miRNA-216b based on therapy for NSCLC. Methods Eighty patients with NSCLC and 40 healthy subjects were enrolled in our study. The SUVmax of the lesion area by PET/CT imaging was calculated. SUVmax represented the highest concentration of 18F-FDG in the lesion. The expression of miRNA-216b in the plasma and fiber bronchoscopic puncture of NSCLC patients was detected by RT qPCR. Then Pearson correlation analysis was used to analyze the correlation between miRNA-216b expression and 18F-FDG uptake in patients with different types of NSCLC. Results Compared with healthy subjects, SUVmax of early adenocarcinoma and advanced adenocarcinoma were increased. Compared with healthy subjects, SUVmax of early squamous and advanced squamous were increased. And the SUVmax content of advanced adenocarcinoma and squamous cell carcinoma was higher than that of early adenocarcinoma and squamous cell carcinoma. Compared with healthy subjects, the expression of miRNA-216b in the plasma of patients with early and advanced adenocarcinoma was reduced, and the expression of miRNA-216b in the plasma of patients with early and advanced squamous cell carcinoma was reduced. Compared with adjacent tissues, the expression of miRNA-216b in early adenocarcinoma tissues and advanced adenocarcinoma tissues was reduced, and the expression in early squamous cell carcinoma and advanced squamous cell carcinoma was reduced. Pearson correlation analysis showed a negative correlation between SUVmax and miRNA-216b (plasma and tissue) in patients with four types of NSCLC. Conclusion miRNA-216b expression was negatively correlated with 18F-FDG uptake in NSCLC. miRNA-216b could be used for the classification and staging of non-small cell lung cancer. 18F-FDG PET/CT may be used to evaluate the therapeutic response in application of miRNA-216b-based cancer treatment.

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Therapeutic Delivery of miR-143 Targeting Tumor Metabolism in Poorly Differentiated Thyroid Cancer Xenografts and Efficacy Evaluation Using ¹⁸F-FDG MicroPET-CT

Cited by 11 publications

References 36 publications

miR-143-Mediated Responses to Betaine Supplement Repress Lipogenesis and Hepatic Gluconeogenesis by Targeting MAT1a and MAPK11

miR-143-Mediated Responses to Betaine Supplement Repress Lipogenesis and Hepatic Gluconeogenesis by Targeting MAT1a and MAPK11

Non-Coding RNAs: Uncharted Mediators of Thyroid Cancer Pathogenesis

The expression of miRNA-216b is negatively correlated with 18F-FDG uptake in non-small cell lung cancer

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Therapeutic Delivery of miR-143 Targeting Tumor Metabolism in Poorly Differentiated Thyroid Cancer Xenografts and Efficacy Evaluation Using 18F-FDG MicroPET-CT

Cited by 11 publications

References 36 publications

miR-143-Mediated Responses to Betaine Supplement Repress Lipogenesis and Hepatic Gluconeogenesis by Targeting MAT1a and MAPK11

miR-143-Mediated Responses to Betaine Supplement Repress Lipogenesis and Hepatic Gluconeogenesis by Targeting MAT1a and MAPK11

Non-Coding RNAs: Uncharted Mediators of Thyroid Cancer Pathogenesis

The expression of miRNA-216b is negatively correlated with 18F-FDG uptake in non-small cell lung cancer

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Therapeutic Delivery of miR-143 Targeting Tumor Metabolism in Poorly Differentiated Thyroid Cancer Xenografts and Efficacy Evaluation Using ¹⁸F-FDG MicroPET-CT