The expression of miRNA-216b is negatively correlated with 18F-FDG uptake in non-small cell lung cancer

Zuo, Mingfei; Yao, Lan; Wen, Liang; Shen, Jianfei; Zhang, Na; Bai, Tian; Huang, Qicheng

doi:10.1186/s12957-021-02376-2

Cited by 8 publications

(7 citation statements)

References 59 publications

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“…LDCT and 18F-deoxyglucose positron emission tomography/computed tomography (18 F-FDG PET/CT) are common imaging methods, but circulating miRNA is highly speci c and less sensitive in differentiating BPNs from MPNs, implying that circulating miRNA helps other imaging methods in diagnosing benign and malignant lung nodules with precision [30]. miRNA combined with PET/CT has been shown to improve the accuracy of NSCLC diagnosis [31]. On the other hand, assays that evaluate circulating miRNA expression pro les are less physically harmful to patients than imaging methods and are successful at screening and diagnosing LC.…”

Section: Discussionmentioning

confidence: 99%

Meta-analysis of the Diagnostic Value of Circulating miRNAs for the Diagnosis of Benign and Malignant Lung Nodules

Hong

Cui

Bai

et al. 2023

Preprint

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Objective A meta-analysis was conducted to assess the impact of miRNAs in circulation on diagnosing benign and malignant pulmonary nodules (BPNs and MPNs). Methods Electronic databases such as Embase, PubMed, Web of Science, and The Cochrane Library were utilized for diagnostic tests of circulating miRNAs to diagnose BPNs and MPNs from the library creation to February 2023. Meta-analysis of the included literature was performed using Stata 16, Meta-Disc 1.4, and Review Manager 5.4 software. This study determined the combined sensitivity, specificity, diagnostic ratio (DOR), positive/negative likelihood ratios (PLR/NLR), as well as value of area under the receiver operating characteristic (ROC) curve. Results This meta-analysis included 14 publications and 17 studies. According to our findings, the pooled sensitivity for miRNA in diagnosing benign and malignant pulmonary nodules was 0.82 [95% CI (0.74, 0.88)], specificity was 0.84 [95% CI (0.79, 0.88)], whereas the DOR was 22.69 [95% CI (13.87, 37.13)], PLR was 5.00 [95% CI (3.87, 6.46)], NLR was 0.22 [95% CI (0.15, 0.32)], and an area under the subject working characteristic curve (AUC) of 0.89 [95% CI (0.86, 0.91)]. Conclusion Circulating miRNAs could be used with sensitivity, specificity, DOR, PLR, NLR, and AUC as biomarkers to diagnose pulmonary nodules (PNs). However, because of the significant heterogeneity of the studies included, more research is needed to determine the optimum miRNA combinations for diagnosing PNs.

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Section: Discussionmentioning

confidence: 99%

Meta-analysis of the Diagnostic Value of Circulating miRNAs for the Diagnosis of Benign and Malignant Lung Nodules

Hong

Cui

Bai

et al. 2023

Preprint

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“…Lung adenocarcinoma (LUAD) is one of the major subtypes of lung cancer that is associated with poor prognosis [ 14 , 15 ]. At present, the literature reports that the occurrence and development of lung adenocarcinoma are closely related to circular RNA [ 16 – 18 ], microRNA [ 19 , 20 ], and N6 methyladenosine [ 21 ]. Some scholars have also found some genes related to the differences and prognosis through biological information analysis [ 15 , 22 – 24 ], for example, EGFR, KRAS, PIK3CA, STEAP1/2, HOXA11, and TCN1.…”

Section: Discussionmentioning

confidence: 99%

UCHL1 acts as a potential oncogene and affects sensitivity of common anti-tumor drugs in lung adenocarcinoma

et al. 2022

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Background Lung adenocarcinoma is the leading cause of cancer death worldwide. Recently, ubiquitin C-terminal hydrolase L1 (UCHL1) has been demonstrated to be highly expressed in many tumors and plays the role of an oncogene. However, the functional mechanism of UCHL1 is unclear in lung adenocarcinoma progression. Methods We analyzed the differential expression of the UCHL1 gene in lung adenocarcinoma and normal lung tissues, and the correlation between the UCHL1 gene and prognosis was also analyzed by the bioinformatics database TCGA. Meanwhile, we detected and analyzed the expression of UCHL1 and Ki-67 protein in a tissue microarray (TMA) containing 150 patients with lung adenocarcinoma by immunohistochemistry (IHC) and clinicopathological characteristics by TCGA database. In vitro experiments, we knocked down the UCHL1 gene of A549 cells and detected the changes in cell migration, invasion, and apoptosis. At the same time, we analyzed the effect of UCHL1 on anti-tumor drug sensitivity of lung adenocarcinoma by a bioinformatics database. In terms of the detection rate of lung adenocarcinoma indicators, we analyzed the impact of UCHL1 combined with common clinical indicators on the detection rate of lung adenocarcinoma through a bioinformatics database. Results In this study, the analysis of UCHL1 protein expression in lung adenocarcinoma proved that obviously higher UCHL1 protein level was discovered in lung adenocarcinoma tissues. The expression of UCHL1 was closely related to poor clinical outcomes. Interestingly, a significantly positive correlation between the expression of UCHL1 and Ki-67-indicated UCHL1 was associated with tumor migration and invasion. Through executing loss of function tests, we affirmed that silencing of UCHL1 expression significantly inhibited migration and invasion of lung adenocarcinoma cells in vitro. Furthermore, lung adenocarcinoma cells with silenced UCHL1 showed a higher probability of apoptosis. In terms of the detection rate of lung adenocarcinoma indicators, we discovered UCHL1 could improve the detection rate of clinical lung adenocarcinoma and affect drug sensitivity. Conclusion In lung adenocarcinoma, UCHL1 promotes tumor migration, invasion, and metastasis by inhibiting apoptosis and has an important impact on the clinical drug treatment of lung adenocarcinoma. In addition, UCHL1 can improve the detection rate of clinical lung adenocarcinoma. Above all, UCHL1 may be a new marker for the diagnosis of lung adenocarcinoma and provide a new target for the treatment of clinical diseases.

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“…LncRNADLGAP1-AS2 regulates miR-503/cyclinD1 to promote nonsmall cell lung cancer cell proliferation [ 35 ]. MiRNA-216b expression was negatively correlated with 18F-FDG uptake in NSCLC [ 36 ]. The overexpression of microRNA-939-5p contributes to cell proliferation and is associated with poor prognosis in glioma [ 37 ].…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Screening and Biological Function Analysis of miRNA and mRNA Related to Lung Adenocarcinoma Based on Bioinformatics Technology

Jia¹,

Ren²,

Liu³

et al. 2022

Journal of Oncology

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Objective. To screen the differentially expressed miRNAs (DEMs) and the differentially expressed gene mRNAs (DEGs) in lung adenocarcinoma (LUAD) from the TCGA database and to explore the relationship between miRNAs and the prognosis of lung adenocarcinoma and their biological functions. Methods. The RNA-seq and miRNA-seq data of lung adenocarcinoma samples were downloaded from the TCGA database for analysis, and the R program was used to screen for differentially expressed miRNAs and mRNAs. Then, the molecular functions, biological processes, cellular components, and signaling pathways involved in the occurrence and development of LUAD were analyzed using the functional accumulation analysis software of GSEA. The relationship between the integrated differentially expressed RNAs was analyzed by miRcode, TargetScan, and miRTarbase databases, and the miRNA-mRNA network was constructed. Result. A total of 516 differentially expressed miRNAs and 5464 differentially expressed mRNAs were identified in LUAD. The GSEA enrichment analysis showed that miRNAs and mRNAs were mainly enriched in extracellular structure organization, external encapsulating structure organization, extracellular matrix organization, and gated channel activity. They were mainly involved in neuroactive ligand-receptor interaction signaling pathway. Some miRNAs and mRNAs in clustering modules were found to be associated with the prognosis of LUAD. Four targeting networks consisting of 22 miRNAs and 531 mRNAs were constructed. Conclusion. The miRNA and mRNA related to the prognosis of LUAD were screened out, which provided a valuable preliminary basis for the follow-upin-depth clinical research and basic experimental research of LUAD.

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The expression of miRNA-216b is negatively correlated with 18F-FDG uptake in non-small cell lung cancer

Cited by 8 publications

References 59 publications

Meta-analysis of the Diagnostic Value of Circulating miRNAs for the Diagnosis of Benign and Malignant Lung Nodules

Meta-analysis of the Diagnostic Value of Circulating miRNAs for the Diagnosis of Benign and Malignant Lung Nodules

UCHL1 acts as a potential oncogene and affects sensitivity of common anti-tumor drugs in lung adenocarcinoma

Screening and Biological Function Analysis of miRNA and mRNA Related to Lung Adenocarcinoma Based on Bioinformatics Technology

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