A study is made of the characterization of the interfacial energy in glass/resin laminates by tensile and flexural tests. Glass/resin laminates, such as slide glass/resin/slide glass, LCD glass/resin/slide glass, and LCD glass/resin/silicon wafer layer structures, are constructed. The effects of the lubricant, mold/substrate materials and resin thickness on the interfacial energy are investigated. The application of lubricant in the laminates critically reduces the interfacial energy during the demolding process. The interfacial energy is determined not only by the mold/resin interface but also by the resin/substrate interface. Although the interfacial energy is influenced by increased surface of micro-sized patterns, the patterns are conserved after the mold is demolded by the introduction of lubricant. A higher interfacial energy increases the adhesive force between the mold and the resin when the resin is thinner, indicating the need for lubricant. In contrast, thinner resin is profitable for designing strong laminate applications.