Theaflavin-3, 3′-Digallate Suppresses RANKL-Induced Osteoclastogenesis and Attenuates Ovariectomy-Induced Bone Loss in Mice

Ai, Zexin; Wu, Yang’ou; Yu, Miao; Li, Jia; Li, Shengjiao

doi:10.3389/fphar.2020.00803

Cited by 11 publications

(9 citation statements)

References 47 publications

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“…OS was evaluated by detecting intracellular ROS generation, malondialdehyde (MDA) levels, and superoxide dismutase (SOD) activity. 25 Intracellular ROS were detected using an ROS Assay Kit (Beyotime) in this study. After treatment, cells in 24-well plates were incubated with 0.1% DCFH-DA diluted in α-MEM at 37°C for 25 min.…”

Section: Detection Of Osmentioning

confidence: 99%

Quercetin Prevents Oxidative Stress-Induced Injury of Periodontal Ligament Cells and Alveolar Bone Loss in Periodontitis

Yu¹,

Fu²,

Wu³

et al. 2021

DDDT

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Purpose Emerging evidence has indicated that oxidative stress (OS) contributes to periodontitis. Periodontal ligament cells (PDLCs) are important for the regeneration of periodontal tissue. Quercetin, which is extracted from fruits and vegetables, has strong antioxidant capabilities. However, whether and how quercetin affects oxidative damage in PDLCs during periodontitis remains unknown. The aim of this study was to assess the effects of quercetin on oxidative damage in PDLCs and alveolar bone loss in periodontitis and underlying mechanisms. Materials and Methods The tissue block culture method was used to extract human PDLCs (hPDLCs). First, a cell counting kit 8 (CCK-8) assay was used to identify the optimal concentrations of hydrogen peroxide (H 2 O 2 ) and quercetin. Subsequently, a 2,7-dichlorodihydrofluorescein diacetate (DCFH-DA) probe, RT-qPCR, Western blotting and other methods were used to explore the effects of quercetin on OS in hPDLCs and the underlying mechanism. Finally, quercetin was administered to mice with periodontitis through gavage, and the effect of quercetin on the level of OS and alveolar bone resorption in these mice was observed by immunofluorescence, microcomputed tomography (micro-CT), hematoxylin and eosin staining (H&E) staining and so on. Results Quercetin at 5 μM strongly activated NF-E2–related factor 2 (NRF2) signaling, alleviated oxidative damage and enhanced the antioxidant capacity of hPDLCs. In addition, quercetin reduced cellular senescence and protected the osteogenic ability of hPDLCs. Finally, quercetin activated NRF2 signaling in the periodontal ligaments, reduced the OS level of mice with periodontitis, and slowed the absorption of alveolar bone in vivo. Conclusion Quercetin can increase the antioxidant capacity of PDLCs and reduce OS damage by activating the NRF2 signaling pathway, which alleviates alveolar bone loss in periodontitis.

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Section: Detection Of Osmentioning

confidence: 99%

Quercetin Prevents Oxidative Stress-Induced Injury of Periodontal Ligament Cells and Alveolar Bone Loss in Periodontitis

Yu¹,

Fu²,

Wu³

et al. 2021

DDDT

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“…A previous study has demonstrated that theaflavins, especially TF-3, attenuate oxidative stress and inflammation (6). TF-3 also has been indicated to decrease the incidence of coronary heart disease, exhibit an advantageous effect on the bone mineral density and the potential to prevent cancer (7)(8)(9). However, the effect of TF-3 in protecting against cigarette smoke extract (CSE)-induced pulmonary emphysema and the mechanism of action remains unclear.…”

Section: Theaflavin-33'-digallate Attenuates Cigarette Smoke Extract-induced Pulmonary Emphysema In Mice By Suppressing Necroptosismentioning

confidence: 99%

Theaflavin‑3,3'‑digallate attenuates cigarette smoke extract‑induced pulmonary emphysema in mice by suppressing necroptosis

Luan

Zhu

et al. 2021

Exp Ther Med

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“…In the process of osteoporosis, the abnormality of bone metabolism is mainly caused by the decrease of bone formation and the increase of bone resorption. In Ai’s study, it reported that TFDG could reduce the activation of osteoclasts by inhibiting oxidative stress, ultimately improving osteoporosis ( Ai et al, 2020 ). However, the effect of TFDG on bone formation is not clear.…”

Section: Discussionmentioning

confidence: 99%

Theaflavin-3,3′-Digallate Promotes the Formation of Osteoblasts Under Inflammatory Environment and Increases the Bone Mass of Ovariectomized Mice

Yang

Hou

et al. 2021

Front. Pharmacol.

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Postmenopausal osteoporosis is a disease of bone mass reduction and structural changes due to estrogen deficiency, which can eventually lead to increased pain and fracture risk. Chronic inflammatory microenvironment leading to the decreased activation of osteoblasts and inhibition of bone formation is an important pathological factor that leads to osteoporosis. Theaflavin-3,3′-digallate (TFDG) is an extract of black tea, which has potential anti-inflammatory and antiviral effects. In our study, we found that TFDG significantly increased the bone mass of ovariectomized (OVX) mice by micro-CT analysis. Compared with OVX mice, TFDG reduced the release of proinflammatory cytokines and increased the expression of osteogenic markers in vivo. In vitro experiments demonstrated that TFDG could promote the formation of osteoblasts in inflammatory environment and enhance their mineralization ability. In this process, TFDG activated MAPK, Wnt/β-Catenin and BMP/Smad signaling pathways inhibited by TNF-α, and then promoted the transcription of osteogenic related factors including Runx2 and Osterix, promoting the differentiation and maturation of osteoblasts eventually. In general, our study confirmed that TFDG was able to promote osteoblast differentiation under inflammatory environment, enhance its mineralization ability, and ultimately increase bone mass in ovariectomized mice. These results suggested that TFDG might have the potential to be a more effective treatment of postmenopausal osteoporosis.

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Theaflavin-3, 3′-Digallate Suppresses RANKL-Induced Osteoclastogenesis and Attenuates Ovariectomy-Induced Bone Loss in Mice

Cited by 11 publications

References 47 publications

Quercetin Prevents Oxidative Stress-Induced Injury of Periodontal Ligament Cells and Alveolar Bone Loss in Periodontitis

Quercetin Prevents Oxidative Stress-Induced Injury of Periodontal Ligament Cells and Alveolar Bone Loss in Periodontitis

Theaflavin‑3,3'‑digallate attenuates cigarette smoke extract‑induced pulmonary emphysema in mice by suppressing necroptosis

Theaflavin-3,3′-Digallate Promotes the Formation of Osteoblasts Under Inflammatory Environment and Increases the Bone Mass of Ovariectomized Mice

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