Quercetin Prevents Oxidative Stress-Induced Injury of Periodontal Ligament Cells and Alveolar Bone Loss in Periodontitis

Abstract

Purpose Emerging evidence has indicated that oxidative stress (OS) contributes to periodontitis. Periodontal ligament cells (PDLCs) are important for the regeneration of periodontal tissue. Quercetin, which is extracted from fruits and vegetables, has strong antioxidant capabilities. However, whether and how quercetin affects oxidative damage in PDLCs during periodontitis remains unknown. The aim of this study was to assess the effects of quercetin on oxidative damage in PDLCs and alveolar bone loss in periodontitis and underlying mechanisms. Materials and Methods The tissue block culture method was used to extract human PDLCs (hPDLCs). First, a cell counting kit 8 (CCK-8) assay was used to identify the optimal concentrations of hydrogen peroxide (H 2 O 2 ) and quercetin. Subsequently, a 2,7-dichlorodihydrofluorescein diacetate (DCFH-DA) probe, RT-qPCR, Western blotting and other methods were used to explore the effects of quercetin on OS in hPDLCs and the underlying mechanism. Finally, quercetin was administered to mice with periodontitis through gavage, and the effect of quercetin on the level of OS and alveolar bone resorption in these mice was observed by immunofluorescence, microcomputed tomography (micro-CT), hematoxylin and eosin staining (H&E) staining and so on. Results Quercetin at 5 μM strongly activated NF-E2–related factor 2 (NRF2) signaling, alleviated oxidative damage and enhanced the antioxidant capacity of hPDLCs. In addition, quercetin reduced cellular senescence and protected the osteogenic ability of hPDLCs. Finally, quercetin activated NRF2 signaling in the periodontal ligaments, reduced the OS level of mice with periodontitis, and slowed the absorption of alveolar bone in vivo. Conclusion Quercetin can increase the antioxidant capacity of PDLCs and reduce OS damage by activating the NRF2 signaling pathway, which alleviates alveolar bone loss in periodontitis.

Quercetin Preserves Oral Cavity Health by Mitigating Inflammation and Microbial Dysbiosis

“…These results further confirm the safety profile, efficacy and health benefits of Quercetin in the oral cavity. Our results concur with previous studies which delivered Quercetin through subcutaneous and intragastric delivery to halt periodontitis and reveal for the first time the effectiveness of oral delivery which is a more clinically plausible and practical application for supplemental use in periodontal practice (76)(77)(78). In fact, after just 2 weeks of Quercetin supplementation, mice exhibited decreased alveolar bone loss compared to vehicle-treated mice.…”

“…In fact, after just 2 weeks of Quercetin supplementation, mice exhibited decreased alveolar bone loss compared to vehicle-treated mice. Most recently, Quercetin was shown to prevent oxidative stress-induced injury of periodontal ligament cells and reduce alveolar bone loss in periodontitis after intragastric delivery (78). Similar beneficial effects of Quercetin have been consistently noted in other immune and inflammatory conditions which share common pathophysiological features with periodontitis (28)(29)(30)(79)(80)(81)(82).…”

PLA Nanofibers for Microenvironmental-Responsive Quercetin Release in Local Periodontal Treatment

“…Wei et al provided evidence of the ability of QUE to alleviate oxidative damage and enhance the antioxidant capacity of periodontal ligament cells through the activation of the NF-E2–related factor 2 (NRF2) signaling pathway. In addition, quercetin reduced the oxidative stress levels of mice with periodontitis [ 14 ].…”

“…Moreover, previous reports have found that quercetin can inhibit the release of inflammatory cytokines in human gingival fibroblasts (HGFs) stimulated by Porphyromonas gingivalis lipopolysaccharide in vitro and alleviate alveolar bone resorption in in vivo animal models [ 14 , 15 , 16 , 17 ]. Although several studies identify QUE as a promising phytochemical to sustain periodontal tissue health, the poor bioavailability, high metabolic rate, and rapid clearance from body of QUE limit its clinical application [ 18 ].…”

Network pharmacology combined with GEO database identifying the mechanisms and molecular targets of Polygoni Cuspidati Rhizoma on Peri-implants

“…Studies have reported the activation of Quercetin on osteoblast formation, as well as stimulating matrix mineralization, calcium deposition, and the expression of ALP, COL1, RUNx-2 and other osteogenic genes 43 , 44 . Yu Wei et al found that quercetin increased the antioxidant capacity of PDLCs by activating NRF2 signaling pathway, alleviated oxidative stress damage, and alleviated alveolar bone loss in periodontitis 45 . Luteolin, flavonoid plant, has potent anti-inflammatory effects both in vitro and in vivo that can effectively inhibit the production of TNF-α, IL-6 and NO in LPS induced macrophage-like cell lines, and luteolin's inhibition of inflammatory cytokines and/or ROS production may lead to the inhibition of osteoclast differentiation 46 .…”