The α-methylene-γ-butyrolactone moiety in dehydrocostus lactone is responsible for cytoprotective heme oxygenase-1 expression through activation of the nuclear factor E2-related factor 2 in HepG2 cells

Jeong, Gil‐Saeng; Pae, Hyun‐Ock; Jeong, Sang Ho; Kim, Youn‐Chul; Kwon, Tae-Oh; Lee, Ho Sub; Kim, Nam-Song; Park, Seok Don; Chung, Hun‐Taeg

doi:10.1016/j.ejphar.2007.02.053

Cited by 40 publications

(33 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It was reported that, the ethanolic extract of Costus species showed significant reduction in glucose, urea, total cholesterol in blood of Albino rats [53]. The elevation of glucose level in the present study could be attributed to destruction of hepatocytes induced by CCl 4 intoxication [54] or decreasing of glycogen contents in hepatocytes [55].…”

Section: Discussionsupporting

confidence: 47%

Hepatoprotective Effect of Costus Roots Extract against Carbon Tetrachloride (CCl4)-Induced Liver Injury in Guinea Pigs

Al-Duais¹,

Al-Awthan²

2017

JLS

View full text Add to dashboard Cite

SC (Saussurea costus) (Asteraceae) is a traditionally known and potent plant which is well considered for its medicinal uses in different indigenous Indian systems of medicine. This study was undertaken to investigate the hepatoprotective effect of SC against liver injury induced by CCl 4 intoxication. Animals were divided into five groups. Group I, served as normal control. Group II received 2 mL CCl 4 /kg b.w. diluted with olive oil, at 1:1 ratio on day 11. Groups III and IV were pre-treated orally respectively with 100 and 200 mg/kg b.w. aqueous roots extract of SC for 10 days followed by subcutaneous injection of CCl 4 (2 mL/kg b.w.), once on day 11. Group V were orally given Liv-52 (100 mg/kg b.w.) once daily for 10 days followed by subcutaneous injection of CCl 4 (2 mL/kg b.w.), once on day 11. Our results show that, the activity of serum hepatic enzymes (ALT, AST, and ALP) were significantly elevated in guinea pigs treated with CCl 4 , while both the SC root extract and Liv-52 reduced significantly these enzymes activity. Also, the levels of glucose, urea and cholesterol were decreased when compared with intoxicated control. Histopathological examination of intoxicated animals showed dilation, hemorrhage, vacuolization, inflammation and necrosis indicating liver damage, while the animals received SC or Liv-52 that showed less pathological effects or normal liver when compared to animals treated with CCl 4 alone. Biochemical and histological results confirm the hepatoprotective effect of aqueous extract of SC.

show abstract

Section: Discussionsupporting

confidence: 47%

Hepatoprotective Effect of Costus Roots Extract against Carbon Tetrachloride (CCl4)-Induced Liver Injury in Guinea Pigs

Al-Duais¹,

Al-Awthan²

2017

JLS

View full text Add to dashboard Cite

show abstract

“…1). 5,24 Opening of any of the three rings in the structure significantly reduced its potential for QR induction. In spite of structural similarity between isoalantolactone and alantolactone, a wide difference in cytotoxicity against several cancer cell lines was reported.…”

Section: Discussionmentioning

confidence: 99%

“…[21][22][23][24] Among the seven sesquiterpenes examined in our previous study, three compounds (alantolactone, isoalantolactone, and 5a-epoxyalantolactone) showed strong QR induction, whereas the other sesquiterpenes were inactive or relatively low in activity. In particular, reduced forms of alantolactone and isoalantolactone did not induce QR, suggesting a significant role of the methylene group in the lactone ring in the activity (Fig.…”

Section: Discussionmentioning

confidence: 99%

Isoalantolactone fromInula heleniumCaused Nrf2-Mediated Induction of Detoxifying Enzymes

Seo

Park

Kim

et al. 2009

Journal of Medicinal Food

View full text Add to dashboard Cite

Our previous study demonstrated that methanolic extract of Inula helenium (Elecampane) has the potential to induce detoxifying enzymes such as NAD(P)H:(quinone acceptor) oxidoreductase 1 (EC 1.6.99.2) (NQO1, QR) activity and glutathione S-transferase (GST) and found isoalantolactone and alantolactone as the active components. In this study we investigated the detoxifying enzyme-inducing potential of isoalantolactone, which is present in I. helenium and has a structure similar to that of alantolactone. The compound induced QR in a dose-dependent manner in both Hepa1c1c7 cells and its mutant BPRc1 cells lacking the arylhydrocarbon receptor translocator. Like with most phase 2 enzyme inducers, other phase 2 detoxifying enzymes, including GST, glutathione reductase, gamma-glutamylcysteine synthetase, and heme oxygenase-1, were also induced by isoalantolactone in a dose-dependent manner in the cultured cells. Furthermore, isoalantolactone caused a proportionate increase in luciferase activity depending upon concentration and exposure time in the reporter assay in which HepG2-C8 cells, transfectants carrying antioxidant response element-luciferase gene, were used. The nuclear translocation of nuclear factor-E2-related factor 2 (Nrf2) was stimulated by the compound and attenuated by phosphatidylinositol 3-kinase inhibitors such as LY294002 and wortmannin. In conclusion, isoalantolactone is a candidate for chemoprevention and acts as potent phase 2 enzyme inducer by stimulating the accumulation of Nrf2 in the nucleus.

show abstract

“…It has been demonstrated that the a-methylene-c-butyrolactone structural unit of dehydrocostus lactone (142) is the moiety responsible for increasing cellular resistance to oxidant injury in HepG2 cells, presumably through Nrf2/ARE-dependent HO-1 expression (Jeong et al, 2007).…”

Section: Other Activitiesmentioning

confidence: 99%

Sesquiterpenoids in subtribe Centaureinae (Cass.) Dumort (tribe Cardueae, Asteraceae): Distribution, 13C NMR spectral data and biological properties

et al. 2013

View full text Add to dashboard Cite

The α-methylene-γ-butyrolactone moiety in dehydrocostus lactone is responsible for cytoprotective heme oxygenase-1 expression through activation of the nuclear factor E2-related factor 2 in HepG2 cells

Cited by 40 publications

References 36 publications

Hepatoprotective Effect of Costus Roots Extract against Carbon Tetrachloride (CCl4)-Induced Liver Injury in Guinea Pigs

Hepatoprotective Effect of Costus Roots Extract against Carbon Tetrachloride (CCl4)-Induced Liver Injury in Guinea Pigs

Isoalantolactone fromInula heleniumCaused Nrf2-Mediated Induction of Detoxifying Enzymes

Sesquiterpenoids in subtribe Centaureinae (Cass.) Dumort (tribe Cardueae, Asteraceae): Distribution, 13C NMR spectral data and biological properties

Contact Info

Product

Resources

About