The zymographic evaluation of gelatinase (MMP-2 and -9) levels in acute and chronic periapical abscesses

Buzoğlu, Hatice Doğan; Ünal, Hasan; Ülger, Celal; Mert, Safak; Küçükyıldırım, Sibel; Er, Nuray

doi:10.1016/j.tripleo.2009.07.014

Cited by 27 publications

(18 citation statements)

References 31 publications

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“…MMP2 participates in the remodeling and resolution of tissue injury (11) and infection clearance (12). On the other hand, MMP9 is a multidomain enzyme functioning in acute and chronic inflammatory condition (13). It is essential for initiating the osteoclastic resorption process by removing the collagenous layer from the bone demineralization (14).…”

Section: Introductionmentioning

confidence: 99%

MMP2 and MMP9 are Associated with Apical Periodontitis Progression and Might be Modulated by TLR2 and MyD88

et al. 2018

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The aim of this study was to evaluate the expression of MMP2 and MMP9 during apical periodontitis (AP) progression in TLR2 (TLR2 KO) and in MyD88 (MyD88 KO) knockout mice compared to wild type (WT) mice. AP was induced in mandibular first molars of TLR2 KO (n= 18), MyD88 KO (n= 18), and WT mice (n= 18). After 7, 21, and 42 days, the animals were euthanized and the jaws were dissected and subjected to histotechnical processing. Subsequent sections were stained by immunohistochemistry and evaluated for detection of MMP2 and MMP9. Statistical analysis of the semi-quantitative analysis of immunohistochemistry was performed using chi-square test (α = 0.05). In the initial periods of AP progression, an increased expression of MMP9 in the TLR2 KO and MyD88 KO mice was observed. In the final periods of AP progression, a reduction of MMP2 expression and an increase of MMP9 expression in the TLR2 KO mice were observed. MMP2 and MMP9 production was modulated for TLR2 and MyD88 during apical periodontitis progression. MMP2 and MMP9 are Associated w i t h A p i c a l P e r i o d o n t i t i s P r o g r e s s i o n a n d M i g h t b e Modulated by TLR2 and MyD88

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Section: Introductionmentioning

confidence: 99%

MMP2 and MMP9 are Associated with Apical Periodontitis Progression and Might be Modulated by TLR2 and MyD88

et al. 2018

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“…MMP-9 and MMP-2 have been identified through immunohistochemistry in experimentally-induced apical periodontitis in animal models where they were proposed to play a role in both, the initiation and progression of apical periodontitis [11,12]. Previous works have demonstrated increased mRNA expression levels of MMP-9 in apical granulomas in comparison to cysts [13,14], as well as MMP-9 activity levels in apical exudates from acute versus apical abscesses [15]. Furthermore, recent preliminary studies reported for the first time that gingival crevicular fluid (GCF) composition changes in AAP-affected teeth, showing increments in MMP-9 activity, frequency of detection of MMP-2 and total protein content in comparison to healthy controls [16,17].…”

Section: Introductionmentioning

confidence: 99%

Pro-oxidant status and matrix metalloproteinases in apical lesions and gingival crevicular fluid as potential biomarkers for asymptomatic apical periodontitis and endodontic treatment response

Dezerega¹,

Madrid

Mundi³

et al. 2012

J Inflamm

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BackgroundOxidative stress and matrix metalloproteinases -9 and -2 are involved in periodontal breakdown, whereas gingival crevicular fluid has been reported to reflect apical status. The aim of this study was to characterize oxidant balance and activity levels of MMP -2 and -9 in apical lesions and healthy periodontal ligament; and second, to determine whether potential changes in oxidant balance were reflected in gingival crevicular fluid from asymptomatic apical periodontitis (AAP)-affected teeth at baseline and after endodontic treatment.MethodsPatients with clinical diagnosis of AAP and healthy volunteers having indication of tooth extraction were recruited. Apical lesions and healthy periodontal ligaments, respectively, were homogenized or processed to obtain histological tissue sections. Matrix metalloproteinase -9 and -2 levels and/or activity were analyzed by Immunowestern blot, zymography and consecutive densitometric analysis, and their tissue localization was confirmed by immunohistochemistry. A second group of patients with AAP and indication of endodontic treatment was recruited. Gingival crevicular fluid was extracted from AAP-affected teeth at baseline, after endodontic treatment and healthy contralateral teeth. Total oxidant and antioxidant status were determined in homogenized tissue and GCF samples. Statistical analysis was performed using STATA v10 software with unpaired t test, Mann-Whitney test and Spearman's correlation.ResultsActivity of MMP-2 and MMP-9 along with oxidant status were higher in apical lesions (p < 0.05). Total oxidant status correlated positively with matrix metalloproteinase-2 and lesion size (p < 0.05). Gingival crevicular fluid showed significantly lower levels of total antioxidant status in diseased teeth at baseline compared to controls and endodontically-treated groups.ConclusionsApical lesions display an oxidant imbalance along with increased activity of matrix metalloproteinase-2 and -9 and might contribute to AAP progression. Oxidant imbalance can also be reflected in GCF from AAP-affected teeth and was restored to normal levels after conservative endodontic treatment. These mediators might be useful as potential biomarkers for chair-side complementary diagnostic of apical status in GCF.

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“…The MMPs are a family of zinc-dependent endopeptidases collectively capable of degrading all extracellular matrix components, including collagen and proteoglycans. The MMPs have been suggested to play an important role in inflammatory conditions of periodontal, pulpal and periapical tissues, as well as dentin mineralization [1]. Tjäderhane et al demonstrated that in periapical inflamemation MMP inhibition increases the lesion growth rate, indicating that MMPs may have previously unknown anti-infective and/or anti-inflammatory properties [17].…”

Section: Introductionmentioning

confidence: 99%

Matrix Matalloproteinase-8 Gene Polymorphism in Chronic Periapical Lesions

Evrosimovska¹,

Dimova²,

Popovska³

et al. 2015

Prilozi

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Background/Aim: Increased levels of matrix metalloproteinase-8 (MMP-8) have been associated with risk of developing chronic periapical lesion (CPL). Gene polymorphisms are contributing factors in the pathogenesis of the inflammation. The aim of this study was to analyze polymorphism in the gene MMP-8 and its association and influence on clinical manifestation of CPL. Methods: A total of 240 unrelated Macedonian subjects were included in the present study. Polymorphism -799 C/T in the gene MMP-8 detected with restriction enzyme BgIII was studied in 120 patients with CPL and 120 controls without any signs of chronic or acute inflammatory process in the jaw. The amplification of the region of selected gene was made with polymerase chain reactionrestriction fragment length polymorphism (PCR-RFLP). Results: Our results showed that there were differences in the allele and genotype frequencies of the MMP8 -799 C/T polymorphisms between patients with CPL and controls (p < 0.05). Also this study suggests that MMP-8 polymorphism -799 C/T was a risk for expression of CPL (OR=8.27<3.44

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The zymographic evaluation of gelatinase (MMP-2 and -9) levels in acute and chronic periapical abscesses

Cited by 27 publications

References 31 publications

MMP2 and MMP9 are Associated with Apical Periodontitis Progression and Might be Modulated by TLR2 and MyD88

MMP2 and MMP9 are Associated with Apical Periodontitis Progression and Might be Modulated by TLR2 and MyD88

Pro-oxidant status and matrix metalloproteinases in apical lesions and gingival crevicular fluid as potential biomarkers for asymptomatic apical periodontitis and endodontic treatment response

Matrix Matalloproteinase-8 Gene Polymorphism in Chronic Periapical Lesions

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