The Zebrafish Information Network (ZFIN): a resource for genetic, genomic and developmental research

Abstract

The Zebrafish Information Network, ZFIN, is a WWW community resource of zebrafish genetic, genomic and developmental research information (http://zfin.org). ZFIN provides an anatomical atlas and dictionary, developmental staging criteria, research methods, pathology information and a link to the ZFIN relational database (http://zfin. org/ZFIN/). The database, built on a relational, object-oriented model, provides integrated information about mutants, genes, genetic markers, mapping panels, publications and contact information for the zebrafish research community. The database is populated with curated published data, user submitted data and large dataset uploads. A broad range of data types including text, images, graphical representations and genetic maps supports the data. ZFIN incorporates links to other genomic resources that provide sequence and ortholog data. Zebrafish nomenclature guidelines and an automated registration mechanism for new names are provided. Extensive usability testing has resulted in an easy to learn and use forms interface with complex searching capabilities.

Genomic structure and embryonic expression of zebrafish lysyl hydroxylase 1 and lysyl hydroxylase 2

“…Consistent with their known roles in collagen biosynthesis, our in situ analyses of lh1 and lh2 demonstrate the expression of these genes in tissues shown previously to express various forms of collagen (Sprague et al, 2001). However, the dynamic patterns of lh1 and lh2 expression in a variety of tissues during zebrafish embryogenesis suggest that these genes do not simply play a housekeeping role, but instead participate in specific developmental events.…”

Analysis of origin and growth of the thyroid gland in zebrafish

“…Both the ctsb and the tg gene were deposited in the ZFIN Expression database (Sprague et al, 2001) as clones zgc:65809 and cb717, respectively. Clones were obtained from RZPD (ctsb; Berlin, Germany) and from the University of Oregon (tg; Eugene, OR).…”

Desarrollo embrionario-larval y tiempo de metamorfosis del pez tropical <i>Xenomelaniris brasiliensis</i> (Pisces: Atherinidae)

“…Se contabilizaron 18 somitos y se observó el canal de circulación vitelino (Fig. 3D), anteriormente reportado por Sprague et al (2001). A las 53 hr: 07 min, se observó completamente definida la circulación del fluido en los canales vitelinos.…”

“…Esta variación esta relacionada con la temperatura y la maduración de los ovocitos, durante el tiempo transcurrido desde la ovulación y a las diferencias entre ejemplares reproductores de una misma especie (Sprague et al 2001). Al respecto White et al (1983) refieren que pueden encontrarse numerosas gotas de aceite en el vitelo de la mayoría de las especies de Atheriniformes.…”

“…Sprague et al (2001), López et al (2002) afirman que las divisiones consecutivas de los blastómeros no están separadas por períodos de crecimiento y los blastómeros resultantes tienen sólo la mitad del tamaño original después de cada división. Durante el proceso de desarrollo se observó la vesícula de Kupffer.…”