The Yeast Arf GTPase-activating Protein Age1 Is Regulated by Phospholipase D for Post-Golgi Vesicular Transport

Benjamin, Jeremy J. R.; Poon, Pak P.; Lewis, Stephen M.; Auger, Andréanne; Wong, Tania; Singer, Richard A.; Johnston, Gerald C.

doi:10.1074/jbc.m110.185108

Cited by 10 publications

(7 citation statements)

References 34 publications

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“…Arl1-His6 and Arf1-His6 were each coexpressed with N -myristoyltransferase in BL21(DE3) bacterial cells and purified following the procedure to isolate Arf1-His6 as described ( Benjamin et al , 2011 ). Myristoylated GTPase was loaded with radioactive GTP, and GAP activity was assessed as described ( Huber et al , 2001 ; Poon et al , 2001 ).…”

Section: Methodsmentioning

confidence: 99%

Dysregulated Arl1, a regulator of post-Golgi vesicle tethering, can inhibit endosomal transport and cell proliferation in yeast

Benjamin

Poon

Drysdale

et al. 2011

MBoC

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Section: Methodsmentioning

confidence: 99%

Dysregulated Arl1, a regulator of post-Golgi vesicle tethering, can inhibit endosomal transport and cell proliferation in yeast

Benjamin

Poon

Drysdale

et al. 2011

MBoC

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“…The Sorting nexin family (Table 3 ) regulates endosomal sorting of internalized proteins through modulating endosomal-to-lysosomal sorting (Carlton et al, 2004 ). Both elevated Arf GTPase (Tables 1 and 3 ) or attenuated Arf GTPase activating protein family (Table 4 ) expression may help maintain Arf GTPase activity which is essential for the recruitment of coat protein complex I and clathrin coat complexes needed for the generation of transport vesicles (Benjamin et al, 2011 ).…”

Section: Resultsmentioning

confidence: 99%

Murine Hyperglycemic Vasculopathy and Cardiomyopathy: Whole-Genome Gene Expression Analysis Predicts Cellular Targets and Regulatory Networks Influenced by Mannose Binding Lectin

Zou¹,

Bonte²,

Pavlov³

et al. 2012

Front. Immun.

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Hyperglycemia, in the absence of type 1 or 2 diabetes, is an independent risk factor for cardiovascular disease. We have previously demonstrated a central role for mannose binding lectin (MBL)-mediated cardiac dysfunction in acute hyperglycemic mice. In this study, we applied whole-genome microarray data analysis to investigate MBL’s role in systematic gene expression changes. The data predict possible intracellular events taking place in multiple cellular compartments such as enhanced insulin signaling pathway sensitivity, promoted mitochondrial respiratory function, improved cellular energy expenditure and protein quality control, improved cytoskeleton structure, and facilitated intracellular trafficking, all of which may contribute to the organismal health of MBL null mice against acute hyperglycemia. Our data show a tight association between gene expression profile and tissue function which might be a very useful tool in predicting cellular targets and regulatory networks connected with in vivo observations, providing clues for further mechanistic studies.

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“…Gcs1p acts together with other ARF-GAPs, Age1p and Age2p, to regulate the small GTPase Arf1p, 12 , 13 which in turn “primes” vesicle formation by recruiting cargo and other regulators of vesicle transport. 14 DAG stimulates the GAP activity of Age1p and Gcs1p in vitro, 13 , 15 and exocytosis defects in GCS1 and AGE2 mutant cells are rescued by the exogenous addition of DAG. 16 Based on these findings, it is proposed that DAG-induced membrane curvature might recruit and activate ARF-GAP activity at the Golgi.…”

Section: Lipid-dependent Vesicle Biogenesis At the Golgi Apparatusmentioning

confidence: 96%

“… 17 (Although this finding does not preclude the possibility that localized increases in DAG levels within the Golgi membrane induce vesicle biogenesis). PI4P levels might also affect the ARF-GAPs Gcs1p and Age1p, which contain general lipid-binding domains that bind PIPs including PI4P; 15 , 16 a homologous domain can also be found within Age2p. These results suggest that ARF-GAP activities might be affected by PIPs.…”

Section: Lipid-dependent Vesicle Biogenesis At the Golgi Apparatusmentioning

confidence: 99%

Vesicle trafficking from a lipid perspective

2012

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The protein cargo transported by specific types of vesicles largely defines the different secretory trafficking pathways operating within cells. However, mole per mole the most abundant cargo contained within transport vesicles is not protein, but lipid. Taking a “lipid-centric” point-of-view, we examine the importance of lipid signaling, membrane lipid organization and lipid metabolism for vesicle transport during exocytosis in budding yeast. In fact, the essential requirement for some exocytosis regulatory proteins can be bypassed by making simple manipulations of the lipids involved. During polarized exocytosis the sequential steps required to generate post-Golgi vesicles and target them to the plasma membrane (PM) involves the interplay of several types of lipids that are coordinately linked through PI4P metabolism and signaling. In turn, PI4P levels are regulated by PI4P kinases, the Sac1p PI4P phosphatase and the yeast Osh proteins, which are homologs of mammalian oxysterol-binding protein (OSBP). Together these regulators integrate the transitional steps required for vesicle maturation directly through changes in lipid composition and organization.

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The Yeast Arf GTPase-activating Protein Age1 Is Regulated by Phospholipase D for Post-Golgi Vesicular Transport

Cited by 10 publications

References 34 publications

Dysregulated Arl1, a regulator of post-Golgi vesicle tethering, can inhibit endosomal transport and cell proliferation in yeast

Dysregulated Arl1, a regulator of post-Golgi vesicle tethering, can inhibit endosomal transport and cell proliferation in yeast

Murine Hyperglycemic Vasculopathy and Cardiomyopathy: Whole-Genome Gene Expression Analysis Predicts Cellular Targets and Regulatory Networks Influenced by Mannose Binding Lectin

Vesicle trafficking from a lipid perspective

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