The Why, Where, Who, How, and What of the vesicular delivery systems

Kapoor, Bhupinder; Gupta, Reena; Gulati, Monica; Singh, Sachin Kumar; Khursheed, Rubiya; Gupta, Manohar Lal

doi:10.1016/j.cis.2019.07.006

Cited by 63 publications

(33 citation statements)

References 167 publications

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“…Often, the initial amount of lipids used in the preparation may be used as an indication of the weight concentration, thus assuming that the loss of materials during the various steps of sample preparation (extrusion, size exclusion chromatography, etc.) could be neglected [ 34 ].…”

Section: Discussionmentioning

confidence: 99%

Light Scattering as an Easy Tool to Measure Vesicles Weight Concentration

2020

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Over the last few decades, liposomes have emerged as promising drug delivery systems and effective membrane models for studying biophysical and biological processes. For all applications, knowing their concentration after preparation is crucial. Thus, the development of methods for easily controlling vesicles concentration would be of great utility. A new assay is presented here, based on a suitable analysis of light scattering intensity from liposome dispersions. The method, tested for extrusion preparations, is precise, easy, fast, non-destructive and uses a tiny amount of sample. Furthermore, the scattering intensity can be measured indifferently at different angles, or even by using the elastic band obtained from a standard spectrofluorimeter. To validate the method, the measured concentrations of vesicles of different matrix compositions and sizes, measured by light scattering with different angles and instruments, were compared to the data obtained by the standard Stewart assay. Consistent results were obtained. The light scattering assay is based on the assessment of the mass fraction lost in the preparation, and can be applied for methods such as extrusion, homogenization, French press and other microfluidic procedures.

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Section: Discussionmentioning

confidence: 99%

Light Scattering as an Easy Tool to Measure Vesicles Weight Concentration

2020

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“…The most common form of vesicular nanoparticle is the liposome (Bunker et al, 2016), a vesicle formed from naturally occurring phospholipids. Other amphiphilic molecules formed into vesicles are, however, also used in drug delivery, including ethosomes (Touitou et al, 2000), niosomes (Marianecci et al, 2014;Khan and Irchhaiya, 2016;Chen S. et al, 2019;Kapoor et al, 2019;Khalkhali et al, 2019;Inglut et al, 2020), polymersomes (Aibani et al, 2020;Khan et al, 2020), exosomes (Antimisiaris et al, 2018;Villa et al, 2019;Chung et al, 2020;Rahmati et al, 2020), ufasomes (Han, 2013), and drimersomes (Nummelin et al, 2017), comprehensive reviews have been written about vesicle formation (Šegota and Durdica, 2006) and application in drug delivery (Kapoor et al, 2019) in a general context. Polymers and lipids can be formed into other structures than micelles or vesicles, for example two different polymers can be used to form core-shell structures (Ramli et al, 2013;Abbott et al, 2017;Chen G. et al, 2018), for example a solid outer shell with a liquid polymer with drug encapsulated inside; solid lipid nanoparticles (Beloqui et al, 2016;Gordillo-Galeano and Mora-Huertas, 2018;Subramaniam et al, 2020), chitosan (Bernkop-Schnürch and Dünnhaupt, 2012), lipoplex (Scheideler et al, 2020) and other lipid-polymer nanoparticles (Date et al, 2018) have also been proposed.…”

Section: Nanomedicinementioning

confidence: 99%

Mechanistic Understanding From Molecular Dynamics Simulation in Pharmaceutical Research 1: Drug Delivery

Bunker

Róg

2020

Front. Mol. Biosci.

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In this review, we outline the growing role that molecular dynamics simulation is able to play as a design tool in drug delivery. We cover both the pharmaceutical and computational backgrounds, in a pedagogical fashion, as this review is designed to be equally accessible to pharmaceutical researchers interested in what this new computational tool is capable of and experts in molecular modeling who wish to pursue pharmaceutical applications as a context for their research. The field has become too broad for us to concisely describe all work that has been carried out; many comprehensive reviews on subtopics of this area are cited. We discuss the insight molecular dynamics modeling has provided in dissolution and solubility, however, the majority of the discussion is focused on nanomedicine: the development of nanoscale drug delivery vehicles. Here we focus on three areas where molecular dynamics modeling has had a particularly strong impact: (1) behavior in the bloodstream and protective polymer corona, (2) Drug loading and controlled release, and (3) Nanoparticle interaction with both model and biological membranes. We conclude with some thoughts on the role that molecular dynamics simulation can grow to play in the development of new drug delivery systems.

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“…The thin layer evaporation technique is one of the simplest and most used methods [120] to prepare liposomes by hydrating lipid films [85,121,122], which involves the encapsulation of active principles in the organic phase (with lipophilic actives) or in the aqueous phase (with hydrophilic ones) during the initial steps of liposomal preparation. However, using this technique, the The most common technique for the preparation of PLGA NP that is able to encapsulate small hydrophilic molecules is the double emulsion technique (w/o/w), which is a modification of the emulsification-solvent evaporation technique [108].…”

Section: Liposomesmentioning

confidence: 99%

Antioxidant and Anti-Inflammatory Properties of Cherry Extract: Nanosystems-Based Strategies to Improve Endothelial Function and Intestinal Absorption

Beconcini

Felice

Fabiano

et al. 2020

Foods

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Cherry fruit has a high content in flavonoids. These are important diet components protecting against oxidative stress, inflammation, and endothelial dysfunction, which are all involved in the pathogenesis of atherosclerosis, which is the major cause of cardiovascular diseases (CVD). Since the seasonal availability of fresh fruit is limited, research has been focused on cherry extract (CE), which also possesses a high nutraceutical potential. Many clinical studies have demonstrated the nutraceutical efficacy of fresh cherries, but only a few studies on CE antioxidant and anti-inflammatory activities have been carried out. Here, the results concerning the antioxidant and anti-inflammatory activities of CE are reviewed. These were obtained by an in vitro model based on Human Umbilical Vein Endothelial Cells (HUVEC). To clarify the CE mechanism of action, cells were stressed to induce inflammation and endothelial dysfunction. Considering that antioxidants’ polyphenol compounds are easily degraded in the gastrointestinal tract, recent strategies to reduce the degradation and improve the bioavailability of CE are also presented and discussed. In particular, we report on results obtained with nanoparticles (NP) based on chitosan derivatives (Ch-der), which improved the mucoadhesive properties of the chitosan polymers, as well as their positive charge, to favor high cellular interaction and polyphenols intestinal absorption, compared with a non-mucoadhesive negative surface charged poly(lactic-co-glycolic) acid NP. The advantages and safety of different nanosystems loaded with natural CE or other nutraceuticals are also discussed.

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The Why, Where, Who, How, and What of the vesicular delivery systems

Cited by 63 publications

References 167 publications

Light Scattering as an Easy Tool to Measure Vesicles Weight Concentration

Light Scattering as an Easy Tool to Measure Vesicles Weight Concentration

Mechanistic Understanding From Molecular Dynamics Simulation in Pharmaceutical Research 1: Drug Delivery

Antioxidant and Anti-Inflammatory Properties of Cherry Extract: Nanosystems-Based Strategies to Improve Endothelial Function and Intestinal Absorption

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