The ways of free radical oxidation correction in the early posttraumatic period after the combined trauma of the chest and hips

Khudobyak, M. M.

doi:10.11603/mcch.2410-681x.2016.v0.i1.6285

Cited by 3 publications

(4 citation statements)

References 2 publications

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“…The results of our previous studies point to the development of tissue hypoxia in early posttraumatic period in case of combined trauma of the chest and both thighs in rats. We also have shown the increasing of reactive oxygen species contents in the blood leukocyte suspension in 24 hours of posttraumatic period with progressive dynamics 10 . Analyzing the own results and published data, we have suggested that damage of the cell plasma membrane is accompanied by their apoptotic death.…”

Section: Introductionmentioning

confidence: 61%

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Mitochondrial mechanisms of apoptosis in case of experimental combined trauma of the chest and both thighs

Krynytska¹,

KHUDOBIAK²,

Vernygorodskyi³

et al. 2018

Arch Balk Med Union

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Mécanismes mitochondriaux de l'apoptose en cas de traumatisme combiné expérimental de la poitrine et des deux cuisses L'objectif de l'étude. Cette étude vise à étudier les caractéristiques de l'apoptose des neutrophiles sanguins en cas de traumatisme combiné de la poitrine et des deux cuisses chez le rat. Méthodes. L'analyse des échantillons cellulaires pour déterminer les espèces réactives de l'oxygène a été évaluée par la méthode de la cytométrie en flux avec un diacétate de 2.7-dichlorodihydrofluorescéine. Le pourcentage de neutrophiles à faible potentiel mitochondrial transmembranaire et le pourcentage de neutrophiles apoptotiques ont été évalués par la méthode de cytométrie en flux avec des kits spécifiques.

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Section: Introductionmentioning

confidence: 61%

“…So, in case of experimental combined trauma of the chest and thighs a "vicious circle" has developed, which is associated with energy deficiency 10 and overproduction of free radicals, on the background of hypoxia. Superoxide anion is produced the most actively by mitochondria.…”

Section: Discussionmentioning

confidence: 99%

Mitochondrial mechanisms of apoptosis in case of experimental combined trauma of the chest and both thighs

Krynytska¹,

KHUDOBIAK²,

Vernygorodskyi³

et al. 2018

Arch Balk Med Union

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“…The results of the study of oxidative processes in the case of combined trauma of the chest and both thighs show that the most intense free-radical processes occur in the lungs on the 7 th and 14 th days of observation. On the 3 rd day of the experiment, the production of mitochondrial superoxide anion radical increases equally in the tissues of the heart and lungs, while in the tissues of the liver this rate increases to a maximum within 3-14 days [18][19][20]. It has been established that acute hyperproduction of reactive oxygen species by leukocytes in 1 day of the experiment causes disorganization of bioelectrical activity of mitochondrial membranes, which is characterized by a dynamic increase in the ratio of leukocyte cells suspension with reduced transmembrane potential and the ratio of leukocytes with signs of apoptosis with a maximum in 7 days [21].…”

Section: Discussionmentioning

confidence: 99%

Structural Organization of Rat Tissues in Experimental Combined Trauma of the Chest and Both Thighs

Mialiuk¹,

Kaskiv²,

Demianchuk³

et al. 2022

IJMMR

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Background. In cases of severe trauma, fractures of long bones are the most often combined with trauma of the chest, head, and abdomen, as well as development of hemorrhagic shock. Therefore, it is reasonable to study the combined trauma of the chest and lower extremities in details, as well as the post-traumatic multiple organ dysfunction especially in early manifestation stage. Objective. The aim of the study was to identify the features of structural organization of the lungs, heart and liver with underlying combined trauma of the chest and both thighs on the 7th day of the post-traumatic period. Methods. The experimental study involved 22 adult nonlinear white male rats with body mass of 200-210 g, kept on a standard diet at animal facility with food and drinking regimes recommended by the standards for laboratory animals. All animals were divided into 2 groups: the control group (1st, n=10), the experimental group (the 2nd) chest trauma and both thighs trauma, observation for 7 days (n=12). The animals of the experimental group were simulated for right-sided closed pneumothorax with a rib fracture by a trocar under thiopental-sodium anesthesia (40 mg/kg of body weight of the rat, intraperitoneally); it was combined with a fracture of the left and right femurs. Skeletal injury was modeled on each thigh that caused a closed fracture by a single dosed blow with a specially designed device. The blow energy was 0.375 J that corresponds to a severe injury. The associated injury was simulated by successive infliction of both injuries. Results. Examination of the liver of animals on the 7th day of the experiment revealed a rapid growth of lesions in their parenchyma. The central veins were poorly visible and contained single erythrocytes; the vessels of medium caliber of myocardial stroma were dilated and blood-filled, which was manifested by the development of perivascular oedema. In the lungs of experimental animals, a moderate decrease of interstitial tissue oedema of the interalveolar septa was present, while cellular infiltration of mast cells, macrophages and lymphocytes also decreased significantly. Conclusions. Multiple organ injuries, which are characterized by structural changes in the liver, heart and lungs in the combined trauma of the thorax and thighs on the 7th day of experiment were evidenced.

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“…It should be noted that in 14 days of observation mitochondrial apoptosis mechanisms are devalued, but increased percentage of FITC Annexin V-positive cells is remained. So, in case of experimental combined trauma of the chest and thighs "vicious circle" have developed, which is associated with energy deficiency 12 and overproduction of free radicals on the background of hypoxia. Superoxide anion the most actively is produced by mitochondria.…”

Section: Indexmentioning

confidence: 99%

Features of leukocytes’ apoptosis and emoxypine succinate efficacy in case of combined trauma of the chest and both thighs in rats

Krynytska

Holovatiuk²,

Shkrobot³

et al. 2019

Bangladesh J Med Sci

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Objective: This study aims to establish features of blood leukocytes' apoptosis and substantiate the efficacy of emoxypine succinate applying in case of combined trauma of the chest and both thighs in rats. Materials and Methods: Analysis of cell samples to determine reactive oxygen species was evaluated by the flow laser cytometry method, using 2.7-dichlorodihydrofluorescein diacetate (Sigma Aldrich, Germany). The number of leukocytes with low mitochondrial transmembrane potential was evaluated by the flow laser cytometry method, using a kit of reagents «MitoScreen» («BD Pharmingen», USA). The number of apoptotic leukocytes was evaluated by the flow laser cytometry method, using a kit of reagents "ANNEXIN V FITC" ("Beckman Coulter", USA). Emoxypine succinate to animals was injected intraperitoneally 1 time per day during 14 days from the first day of experiment in the dosage of 40 mg/kg. Results and Discussion: It was established the progressive, statistically significant increasing of Annexin V-positive cells percentage from the first day of the combined trauma of the chest and both thighs in rats with the highest values within 7-14 days of observation. On 28 day of experiment the reduction of apoptotic white blood cells percentage by 7.7% than the findings on 14 day was observed, but it remained 33.3% higher than control. The analysis of data in case of emoxypine succinate applying indicates that production of reactive oxygen species by leukocytes began to decline after 3 days of experiment and continued to decrease with maximum of action on 7 day. On 28 day of experiment the production of reactive oxygen species by leukocytes has decreased by 39.8 %; the percentage of leukocytes with low transmembrane potential has decreased by 34.6 % vs rats without medical treatment. At the same time the dynamics of FITC Annexin V-positive leukocytes changes in case of combined trauma of the chest and both thighs in rats and emoxypine succinate applying on 28 day of experiment has decreased by 16.7 % vs rats without medical treatment. Conclusion: One of important signaling pathways of apoptosis triggering in case of experimental combined trauma of the chest and both thighs is reactive oxygen species overproduction and disruption of the mitochondrial inner membrane due to the decreasing of transmembrane potential in 3-7 days of observation. Emoxypine succinate applying in post-traumatic period has a positive effect, characterized by decreasing of the production of reactive oxygen species, the percentage of leukocyte with low mitochondrial transmembrane potential and the percentage of FITC Annexin V-positive cells of leukocyte suspension. But the dynamics of FITC Annexin V-positive leukocytes changes leads us to believe that in the initiation and implementation of cell death in case of combined trauma apart mitochondrial, there are other mechanisms.

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The ways of free radical oxidation correction in the early posttraumatic period after the combined trauma of the chest and hips

Cited by 3 publications

References 2 publications

Mitochondrial mechanisms of apoptosis in case of experimental combined trauma of the chest and both thighs

Mitochondrial mechanisms of apoptosis in case of experimental combined trauma of the chest and both thighs

Structural Organization of Rat Tissues in Experimental Combined Trauma of the Chest and Both Thighs

Features of leukocytes’ apoptosis and emoxypine succinate efficacy in case of combined trauma of the chest and both thighs in rats

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