The Way to High Volume Fabrication of Lab-on-a-Chip Devices—A Technological Approach for Polymer Based Microfluidic Systems with Integrated Active Valves and Pumps

Rupp, Jochen; Schmidt, Manuela; Günther, Bettina; Stumber, Michael; Zinober, Sven; Müller-Fiedler, Roland; Alabsi, Bashir; Rothacher, Peter; Müller, Claas; Reinecke, Holger; Zengerle, Roland; Daub, Martina

doi:10.4071/1551-4897-6.4.198

Cited by 7 publications

(12 citation statements)

References 10 publications

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“…24 This benchmark was exceeded by far and verifies sufficient bond strength of laser welded COP-TPS-COP multilayer stacks. Since the barrier properties of PC are not sufficient 11 for long-term storage, the reagent reservoirs were coated with layers of Al 2 O 3 in a PEALD (Plasma-Enhanced Atomic Layer Deposition) process.…”

Section: High Temperature Storage and Surface Coatingmentioning

confidence: 88%

“…Rupp et al reported a tensile strength of σ ≈ 2.0 N mm −2 for PC-TPU-PC composites. 24 This benchmark was exceeded by far and verifies sufficient bond strength of laser welded COP-TPS-COP multilayer stacks.…”

Section: Tensile Strengthmentioning

confidence: 88%

“…1. [22][23][24][25] In this work, this assembly concept is extended to the novel COP/TPS material combination. transparent PC, COP), a flexible membrane (e.g.…”

Section: Storage Concept and Assemblymentioning

confidence: 99%

“…22 Laser welding of PC/TPU composites was shown by Rupp et al in order to enable pressure driven microfluidic operations. [22][23][24][25] In this work, this assembly concept is extended to the novel COP/TPS material combination. COP was chosen because of its excellent barrier properties.…”

Section: Storage Concept and Assemblymentioning

confidence: 99%

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Liquids on-chip: direct storage and release employing micro-perforated vapor barrier films

et al. 2015

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Liquids on-chip describes a reagent storage concept for disposable pressure driven Lab-on-Chip (LoC) devices, which enables liquid storage in reservoirs without additional packaging. On-chip storage of liquids can be considered as one of the major challenges for the commercial break through of polymer-based LoC devices. Especially the ability for long-term storage and reagent release on demand are the most important aspects for a fully developed technology. On-chip storage not only replaces manual pipetting, it creates numerous advantages: fully automated processing, ease of use, reduction of contamination and transportation risks. Previous concepts for on-chip storage are based on liquid packaging solutions (e.g. stick packs, blisters, glass ampoules), which implicate manufacturing complexity and additional pick and place processes. That is why we prefer on-chip storage of liquids directly in reservoirs. The liquids are collected in reservoirs, which are made of high barrier polymers or coated by selected barrier layers. Therefore, commonly used polymers for LoC applications as cyclic olefin polymer (COP) and polycarbonate (PC) were investigated in the context of novel polymer composites. To ensure long-term stability the reservoirs are sealed with a commercially available barrier film by hot embossing. The barrier film is structured by pulsed laser ablation, which installs rated break points without affecting the barrier properties. A flexible membrane is actuated through pneumatic pressure for reagent release on demand. The membrane deflection breaks the barrier film and leads to efficient cleaning of the reservoirs in order to provide the liquids for further processing.

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Section: High Temperature Storage and Surface Coatingmentioning

confidence: 88%

Section: Tensile Strengthmentioning

confidence: 88%

“…1. [22][23][24][25] In this work, this assembly concept is extended to the novel COP/TPS material combination. transparent PC, COP), a flexible membrane (e.g.…”

Section: Storage Concept and Assemblymentioning

confidence: 99%

Section: Storage Concept and Assemblymentioning

confidence: 99%

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Liquids on-chip: direct storage and release employing micro-perforated vapor barrier films

et al. 2015

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“…The upper and lower bulk layers can be structured using high throughput fabrication processes such as injection molding. 20 All three layers are joined together in one laser welding step (Fig. 3) creating a mechanically stable assembly which shows no leakage even at a hydraulic pressure of 0.90 MPa, significantly higher than the control and hydraulic pressures used for operation (10-50 kPa).…”

Section: Micropump Concept and Assemblymentioning

confidence: 99%

Rapid microarray processing using a disposable hybridization chamber with an integrated micropump

et al. 2012

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We present a disposable microarray hybridization chamber with an integrated micropump to speed up diffusion based reaction kinetics by generating convective flow. The time-to-result for the hybridization reaction was reduced from 60 min (standard protocol) down to 15 min for a commercially available microarray. The integrated displacement micropump is pneumatically actuated. It includes two active microvalves and is designed for low-cost, high volume manufacturing. The setup is made out of two microstructured polymer parts realized in polycarbonate (PC) separated by a 25 μm thermoplastic elastomer (TPE) membrane. Pump rate can be controlled between 0.3 μl s(-1) and 5.7 μl s(-1) at actuation frequencies between 0.2 Hz and 8.0 Hz, respectively.

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Fusing MEMS technology with lab-on-chip: nanoliter-scale silicon microcavity arrays for digital DNA quantification and multiplex testing

et al. 2020

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We report on the development of a microfluidic multiplexing technology for highly parallelized sample analysis via quantitative polymerase chain reaction (PCR) in an array of 96 nanoliter-scale microcavities made from silicon. This PCR array technology features fully automatable aliquoting microfluidics, a robust sample compartmentalization up to temperatures of 95 °C, and an application-specific prestorage of reagents within the 25 nl microcavities. The here presented hybrid silicon–polymer microfluidic chip allows both a rapid thermal cycling of the liquid compartments and a real-time fluorescence read-out for a tracking of the individual amplification reactions taking place inside the microcavities. We demonstrate that the technology provides very low reagent carryover of prestored reagents < 6 × 10−2 and a cross talk rate < 1 × 10−3 per PCR cycle, which facilitate a multi-targeted sample analysis via geometric multiplexing. Furthermore, we apply this PCR array technology to introduce a novel digital PCR-based DNA quantification method: by taking the assay-specific amplification characteristics like the limit of detection into account, the method allows for an absolute gene target quantification by means of a statistical analysis of the amplification results.

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The Way to High Volume Fabrication of Lab-on-a-Chip Devices—A Technological Approach for Polymer Based Microfluidic Systems with Integrated Active Valves and Pumps

Cited by 7 publications

References 10 publications

Liquids on-chip: direct storage and release employing micro-perforated vapor barrier films

Liquids on-chip: direct storage and release employing micro-perforated vapor barrier films

Rapid microarray processing using a disposable hybridization chamber with an integrated micropump

Fusing MEMS technology with lab-on-chip: nanoliter-scale silicon microcavity arrays for digital DNA quantification and multiplex testing

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