Rapid microarray processing using a disposable hybridization chamber with an integrated micropump

Rupp, Jochen; Schmidt, Manuela; Münch, Susanne; Cavalar, Markus; Steller, Ulf; Steigert, Jürgen; Stumber, Michael; Dorrer, Christian; Rothacher, Peter; Zengerle, Roland; Daub, Martina

doi:10.1039/c2lc21110f

Cited by 30 publications

(32 citation statements)

References 21 publications

(22 reference statements)

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“…1. [22][23][24][25] In this work, this assembly concept is extended to the novel COP/TPS material combination. transparent PC, COP), a flexible membrane (e.g.…”

Section: Storage Concept and Assemblymentioning

confidence: 99%

Liquids on-chip: direct storage and release employing micro-perforated vapor barrier films

et al. 2015

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Liquids on-chip describes a reagent storage concept for disposable pressure driven Lab-on-Chip (LoC) devices, which enables liquid storage in reservoirs without additional packaging. On-chip storage of liquids can be considered as one of the major challenges for the commercial break through of polymer-based LoC devices. Especially the ability for long-term storage and reagent release on demand are the most important aspects for a fully developed technology. On-chip storage not only replaces manual pipetting, it creates numerous advantages: fully automated processing, ease of use, reduction of contamination and transportation risks. Previous concepts for on-chip storage are based on liquid packaging solutions (e.g. stick packs, blisters, glass ampoules), which implicate manufacturing complexity and additional pick and place processes. That is why we prefer on-chip storage of liquids directly in reservoirs. The liquids are collected in reservoirs, which are made of high barrier polymers or coated by selected barrier layers. Therefore, commonly used polymers for LoC applications as cyclic olefin polymer (COP) and polycarbonate (PC) were investigated in the context of novel polymer composites. To ensure long-term stability the reservoirs are sealed with a commercially available barrier film by hot embossing. The barrier film is structured by pulsed laser ablation, which installs rated break points without affecting the barrier properties. A flexible membrane is actuated through pneumatic pressure for reagent release on demand. The membrane deflection breaks the barrier film and leads to efficient cleaning of the reservoirs in order to provide the liquids for further processing.

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“…1. [22][23][24][25] In this work, this assembly concept is extended to the novel COP/TPS material combination. transparent PC, COP), a flexible membrane (e.g.…”

Section: Storage Concept and Assemblymentioning

confidence: 99%

Liquids on-chip: direct storage and release employing micro-perforated vapor barrier films

et al. 2015

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“…PDMS is a common valve material because it is easy to mold; however, it is prone to nonspecific adsorption of proteins and permeation of hydrophobic molecules [22], which is problematic for bioanalytical applications and nonideal for valves. In response to this disadvantage, other materials (fluoroelastomers [23–25] and thermoplastic elastomer [26]) have been explored as valve membranes in conjunction with rigid fluidic substrates of cyclic olefin copolymer, poly(methyl methacrylate), or glass. Fluoroelastomers, while resistant to nonspecific adsorption, are normally opaque and difficult to bond.…”

Section: Introductionmentioning

confidence: 99%

Microfluidic valves made from polymerized polyethylene glycol diacrylate

Rogers

Oxborrow

Anderson

et al. 2014

Sensors and Actuators B: Chemical

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Pneumatically actuated, non-elastomeric membrane valves fabricated from polymerized polyethylene glycol diacrylate (poly-PEGDA) have been characterized for temporal response, valve closure, and long-term durability. A ~100 ms valve opening time and a ~20 ms closure time offer valve operation as fast as 8 Hz with potential for further improvement. Comparison of circular and rectangular valve geometries indicates that the surface area for membrane interaction in the valve region is important for valve performance. After initial fabrication, the fluid pressure required to open a closed circular valve is ~50 kPa higher than the control pressure holding the valve closed. However, after ~1000 actuations to reconfigure polymer chains and increase elasticity in the membrane, the fluid pressure required to open a valve becomes the same as the control pressure holding the valve closed. After these initial conditioning actuations, poly-PEGDA valves show considerable robustness with no change in effective operation after 115,000 actuations. Such valves constructed from non-adsorptive poly-PEGDA could also find use as pumps, for application in small volume assays interfaced with biosensors or impedance detection, for example.

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“…7 The main advantage of these technologies (namely, CD, DVD, and Blu-Ray) is that they are mass-produced for the consumer electronic market with highquality standards and cost-effective prices. The procedures include ow hybridization in various types of reservoirs, such as double-spiral, 11 channel, 12,13 or chamber, 14 and all of them were combined with uorescence detection. These systems show a higher sensitivity and working capacity (e.g.…”

Section: Introductionmentioning

confidence: 99%

Isothermal solid-phase recombinase polymerase amplification on microfluidic digital versatile discs (DVDs)

et al. 2015

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A new advancement in massive DNA-based screening in limited-resource settings is demonstrated through the incorporation of easy-to-fabricate microfluidic chambers on digital versatile discs (DVDs) to perform isothermal recombinase polymerase amplification (RPA) in a microarray format. Standard un-modified DVD discs and commercial drives are used for the low-cost detection method. DNA primers were printed in a microarray format on the polycarbonate surfaces of DVDs with integrated control spots to guarantee the absence of false-negatives and false-positives. The solid-phase amplification assay, including the washing protocols and development reaction, was performed by the dispensation of solutions through the inlet and by controlling the flow-movement by DVD drive centrifugation. The final disc with reaction products was inserted into a DVD player and microarray images were captured and automatically processed. This simple approach was applied for the screening of genetically modified organisms (GMOs) in food samples. The limit of detection was 7 mg g À1 , which is well below the EU regulation limit for GMOs in food products. Therefore, the only required materials for food safety monitoring were standard store-bought DVDs, plastic chambers, tips, pipettes, an oven, and a standard DVD drive. The proposed strategy allows an integrated microarray system with low manipulation, reduced sample volume, and portability, which are beneficial for low-resource settings.

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Rapid microarray processing using a disposable hybridization chamber with an integrated micropump

Cited by 30 publications

References 21 publications

Liquids on-chip: direct storage and release employing micro-perforated vapor barrier films

Liquids on-chip: direct storage and release employing micro-perforated vapor barrier films

Microfluidic valves made from polymerized polyethylene glycol diacrylate

Isothermal solid-phase recombinase polymerase amplification on microfluidic digital versatile discs (DVDs)

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