The water extract of Omija protects H9c2 cardiomyoblast cells from hydrogen peroxide through prevention of mitochondrial dysfunction and activation of caspases pathway

Park, Channy; So, Hong-Seob; Shin, Sae-Ron; Choi, Jin-Sung; Lee, In; Kim, Jin‐Kyung; Chung, Sung Tae; Park, Raekil

doi:10.1002/ptr.2028

Cited by 11 publications

(4 citation statements)

References 36 publications

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“…As one of the key effectors, caspase-3 is initiated by caspase-9 and involved in the mitochondria-mediated pathway. Previous studies have shown that oxidative stress induced the activation of caspase-9 and caspase-3 in cell lines [Park et al, 2007]. Consistent with these results, we demonstrated that the induction of apoptosis induced by A/R was accompanied by an increase of caspase-9 and caspase-3 activities.…”

Section: Discussionsupporting

confidence: 92%

Ganoderma atrum polysaccharide protects cardiomyocytes against anoxia/reoxygenation‐induced oxidative stress by mitochondrial pathway

Nie

Chen

et al. 2010

J of Cellular Biochemistry

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It is now well established that oxidative stress plays a causative role in the pathogenesis of anoxia/reoxygenation (A/R) injury. Ganoderma atrum polysaccharide (PSG-1), the most abundant component isolated from G. atrum, has been shown to possess potent antioxidant activity. The goals of this study were to investigate the effect of PSG-1 against oxidative stress induced by A/R injury and the possible mechanisms in cardiomyocytes. In this work, primary cultures of neonatal rat cardiomyocytes pretreated with PSG-1 were subjected to A/R and subsequently monitored for cell viability by the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay. The levels of intracellular reactive oxygen species (ROS), apoptosis, and mitochondrial membrane potential (Deltapsi(m)) were determined by flow cytometry. Western blot analysis was used to measure the expression of cytochrome c, Bcl-2 family, and manganese superoxide dismutase (MnSOD) proteins, and the activities of caspase-3 and caspase-9 were determined by a colorimetric method. The results showed that PSG-1 protected against cell death caused by A/R injury in cardiomyocytes. PSG-1 reduced the A/R-induced ROS generation, the loss of mitochondrial membrane potential (Deltapsi(m)), and the release of cytochrome c from the mitochondria into cytosol. PSG-1 inhibited the A/R-stimulated activation of caspase-9 and caspase-3 and alteration of Bcl-2 family proteins. Moreover, PSG-1 significantly increased the protein expression of MnSOD in cardiomyocytes. These findings suggest that PSG-1 significantly attenuates A/R-induced oxidative stress and improves cell survival in cardiomyocytes through mitochondrial pathway.

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Section: Discussionsupporting

confidence: 92%

Ganoderma atrum polysaccharide protects cardiomyocytes against anoxia/reoxygenation‐induced oxidative stress by mitochondrial pathway

Nie

Chen

et al. 2010

J of Cellular Biochemistry

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“…The antioxidant activity of many extracts and constituents from medicinal herbs has been widely documented in vivo and in vitro (Park et al, 2007;Piccinelli et al, 2004;Shao et al, 2001). Allium chinense G. Don (Liliaceae), one of the original plants of a famous Chinese crude drug 'Xiebai', has been used routinely in some traditional Chinese preparations for the treatment of stenocardia, arteriosclerosis, so-called stagnant blood (Peng et al, 1996a).…”

Section: Introductionmentioning

confidence: 99%

Protective effects of steroids from Allium chinense against H₂O₂‐induced oxidative stress in rat cardiac H9C2 cells

Ren

Qiao

Yang

et al. 2009

Phytotherapy Research

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Allium chinense, a traditional herbal medicine, has been used for the treatment of cardiovascular diseases for hundreds of years. In this study, A. chinense steroids (ACSs) including three steroidal glycosides and their parent aglycones were isolated from the bulbs of A. chinense. For the first time, their cardioprotective effects were evaluated in cultured rat cardiac H9C2 cells by pretreatment with ACSs for 24 h before exposure to 0.2 mm H(2)O(2). The results showed the cell viability decreased markedly when H9C2 cells were incubated with 0.2 mm H(2)O(2) alone for 2 h, while the cell lipid peroxidation (estimated by the excessive production of nitric oxide and malondialdehyde) and intracellular free calcium concentration ([Ca(2+)](i)) increased significantly. The addition of 20 microm (below the toxic concentration) of ACSs notably attenuated the cellular injury induced by H(2)O(2). The effects of ACSs in our experiments were similar to those of nimodipine, a clinically applied calcium channel blocker. Preliminary analysis of the structure-activity relationship indicated that ACSs with a spirostane-type skeleton exhibited stronger protection than that with a furostane-type skeleton, and glycosylation of the steroids could substantially lower the protective activities. The above results suggested the protective effects of steroids originated from A. chinense on the oxidative injury of H9C2 cells and ACSs may have potential for preventing cardiac injuries induced by oxidative stress.

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“…H9c2 is a clonal cell line derived from embryonic rat heart tissue (Kimes and Brandt, 1976) and exhibits many of the properties of cardiac muscle, including electrophysiology, ion channels and receptors (Fujita et al, 2006). Despite not being fully differentiated, H9c2 cells have been used frequently as a model of cardiac cells in several studies (Kim et al, 2006;Park et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Effect of Flavonoids on Daunorubicin‐induced Toxicity in H9c2 Cardiomyoblasts

Mojžišová

Šarišský

Mirossay

et al. 2008

Phytotherapy Research

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The water extract of Omija protects H9c2 cardiomyoblast cells from hydrogen peroxide through prevention of mitochondrial dysfunction and activation of caspases pathway

Cited by 11 publications

References 36 publications

Ganoderma atrum polysaccharide protects cardiomyocytes against anoxia/reoxygenation‐induced oxidative stress by mitochondrial pathway

Ganoderma atrum polysaccharide protects cardiomyocytes against anoxia/reoxygenation‐induced oxidative stress by mitochondrial pathway

Protective effects of steroids from Allium chinense against H₂O₂‐induced oxidative stress in rat cardiac H9C2 cells

Effect of Flavonoids on Daunorubicin‐induced Toxicity in H9c2 Cardiomyoblasts

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The water extract of Omija protects H9c2 cardiomyoblast cells from hydrogen peroxide through prevention of mitochondrial dysfunction and activation of caspases pathway

Cited by 11 publications

References 36 publications

Ganoderma atrum polysaccharide protects cardiomyocytes against anoxia/reoxygenation‐induced oxidative stress by mitochondrial pathway

Ganoderma atrum polysaccharide protects cardiomyocytes against anoxia/reoxygenation‐induced oxidative stress by mitochondrial pathway

Protective effects of steroids from Allium chinense against H2O2‐induced oxidative stress in rat cardiac H9C2 cells

Effect of Flavonoids on Daunorubicin‐induced Toxicity in H9c2 Cardiomyoblasts

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Protective effects of steroids from Allium chinense against H₂O₂‐induced oxidative stress in rat cardiac H9C2 cells