The water-deficit stress- and red-rot-related genes in sugarcane

Abstract

Sugarcane is an important international commodity as a valuable agricultural crop especially in developing countries. Sequencing was carried out to generate >35,000 expressed sequence tags (ESTs) from healthy as well as red-rot-infected tissue of Indian subtropical variety of sugarcane. Subsequent clustering with existing sugarcane ESTs in public databases identified 4,087 clusters, including 85 clusters that preferentially express upon Colletotrichum falcatum (red-rot) infection, which were previously unreported. Real-time reverse transcription-PCR profiling of selected EST clusters identified several sugarcane clusters that show differential expression in response to biotic and abiotic stress conditions. Twenty-five stress-related clusters showed >2-fold relative expression during water-deficit stress in sugarcane. Similarly, EST clusters could be identified, which exhibit association with red-rot disease when assessed in red-rot-susceptible and red-rot-resistant varieties of sugarcane. Such EST clusters are good candidates for in-depth analysis to elucidate stress-responsive pathways in sugarcane and facilitate genetic manipulation to tailor this crop for tolerance to various stresses.

Differential gene expression in drought-tolerant sugarcane roots

“…In sugarcane under water stress, kinase receptors are involved in the signal transduction response to stress (Gupta et al, 2010). In Arabidopsis under some abiotic, biotic, and hormonal stress treatments, these receptors are involved in various signaling processes (Chae et al, 2009).…”

Identification of Water Deficit Stress Upregulated Genes in Sugarcane

“…In sugarcane, suppression subtractive hybridization (SSH), cDNA microarrays, qRT-PCR, and SUCEST data mining have been used to study responses to oxidative (Kurama et al 2002), aluminum (Watt 2003), cold (Nogueira et al 2003(Nogueira et al , 2005, drought stress (Rocha et al 2007), and water deficit and biotic stress (Gupta et al 2009). …”

Transcriptomics of Sugarcane Osmoprotectants Under Drought

“…Rocha et al (2007) in a cDNA microarrays approach to profile expression of 1,545 genes involved in signaling processes in plants submitted to drought, phosphate starvation, herbivory and N 2 -fixing endophytic bacteria, identified 485 differentially expressed candidates after exposition of the plants to water shortage. In another approach Gupta et al (2010) using cDNA libraries associated to the RTqPCR validation of drought related genes, revealed differences greater than 2-fold regarding the expression of given genes during dehydration stress. The most recent report was carried out by Iskandar et al (2011) that investigated whether the degree of expression of eight stress-related genes -P5CS, OAT, AS, PST5, TF1, LEA, POX and dehydrin -was correlated with the sucrose content in the sugarcane culm, and whether such genes were also responsive to water deficit stress.…”