The Virulence of Spirochaeta Pallida in Culture

Gammel, John A.

doi:10.1001/archderm.1931.03880210032003

Cited by 13 publications

(4 citation statements)

References 1 publication

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“…Steinhardt observed motile treponemes for 25 days in a hanging drop preparation of rabbit testicular tissue (13). Similar extensions of survival were reported by Shaffer, using normal rabbit tissue, and by Gammel and Ecker, using syphilitic rabbit tissue (3,11).…”

supporting

confidence: 75%

Treponema pallidum (Nichols strain) in tissue cultures: cellular attachment, entry, and survival

Fitzgerald¹,

Miller²,

Sykes³

1975

Infect Immun

102

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The interaction of Treponema pallidum (Nichols strain) with cultured cells was investigated under aerobic conditions. Cell monolayers derived from rabbit testicular tissue extended the survival of treponemes as indicated by active motility. Large numbers of organisms rapidly attached to cultured cells. Within 3 h, one to twelve actively motile treponemes were attached to 25 to 50% of the cells. In addition, T. pallidum attained intracellularity as early as 30 min after inoculation of the cell monolayers. In sharp contrast, T. phagedenis biotype Reiter and T. denticola did not attach and did not enter cultured cells. Most importantly, intracellular and/or attached T. pallidum retained virulence for at least 24 h. Similar observations of attachment and retention of virulence were detected with ME-180, a cell line derived from a human cervical carcinoma. Preliminary studies with superoxide dismutase indicated that this enzyme prolonged treponemal motility and retention of virulence in the presence of cultured cells. These data provide guidelines for further investigations of in vitro cultivation of T. pallidum.

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supporting

confidence: 75%

Treponema pallidum (Nichols strain) in tissue cultures: cellular attachment, entry, and survival

Fitzgerald¹,

Miller²,

Sykes³

1975

Infect Immun

102

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“…Borrelia is very difficult to cultivate in initial culture and cannot consistently be maintained in serial passage. Although there are many so-called culture strains of T. pallidum, it is doubtful whether virulent strains of the organism have ever been cultured successfully in vitro (63,93). L. icterohaemorrhagiae (140) and Borrelia (152) have been grown without loss of virulence in the chick embryo.…”

Section: Cultivation and Preservationmentioning

confidence: 99%

“…Variations in virulence. There are strains of spirochetes of different degrees of virulence (63,143,219). As with other microorganisms, virulence is gradually lost upon cultivation in vitro and often regained upon animal passage.…”

mentioning

confidence: 99%

Characteristics of Pathogenic Spirochetes and Spirochetoses With Special Reference to the Mechanisms of Host Resistance

Stavitsky¹

1948

Bacteriological Reviews

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Unless otherwise indicated, data for leptospiras or leptospirosis refer to the species Leptospira icterohaemorrhagiae and diseases of man and animals caused by this species. 3 The reader unacquainted with spirochetes and spirochetoses is referred to any standard textbook of bacteriology for a brief description of these organisms and diseases. Following are the genera and major species of spirochetes and the diseases they cause: Treponema pallidum-syphilis; Treponema cuniculi-rabbit venereal spirochetosis; Treponema pertenue-yaws; Lepto8pira icterohaemorrhagiae-infectious jaundice of Weil's disease in man and animals; Leptospira canicola-leptospirosis in dogs and man; Borrelia duttoni, novyi, and recurrentis, African, American and European relapsing fevers, respectively, in man; Borrelia anserinum-avian spirochetosis. 204 [VOL. 12

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“…Bessemans and de Geest (1928) grew T. pallidum in media with rabbit serum and in ascitic fluid albeit virulence was lost quickly. Extensive work was carried out by Gammel and Ecker (1931) to maintain the survival of the organisms under variable conditions. Although the motility of the organisms was observed for several days, virulence disappeared in 48 hours.…”

mentioning

confidence: 99%

Investigations on in vitro survival and virulence of T. pallidum under aerobiosis.

Rathlev¹

1975

Sexually Transmitted Infections

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The Virulence of Spirochaeta Pallida in Culture

Cited by 13 publications

References 1 publication

Treponema pallidum (Nichols strain) in tissue cultures: cellular attachment, entry, and survival

Treponema pallidum (Nichols strain) in tissue cultures: cellular attachment, entry, and survival

Characteristics of Pathogenic Spirochetes and Spirochetoses With Special Reference to the Mechanisms of Host Resistance

Investigations on in vitro survival and virulence of T. pallidum under aerobiosis.

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