The Viability of Autologous Fat Grafts Harvested With the LipiVage System

Abstract

This study evaluates the viability of adipose aspirates harvested with the LipiVage system (Genesis Biosystems Inc, Lewisville, TX), a newly developed fat harvesting device, and determines a potentially preferred method for possible large-quantity fat graft harvesting. Adipose aspirates were harvested with the LipiVage system from the abdomen of 16 female patients (group 1, n = 8) according to the instruction by the manufacturer and with conventional liposuction (group 2, n = 8). Samples from conventional liposuction were spun at 50 g for 10 minutes and the resulting middle layer of fat was collected. All fat graft samples were evaluated with trypan blue vital staining for viable adipocyte count, glycerol-3-phosphatase dehydrogenase (G3PDH) assay for intracellular enzyme activity, and histology. In this study, group 1 had significantly higher viable adipocyte count than group 2 had (3.7 +/- 0.64 versus 2.37 +/- 0.56 x 10(6) /mL, P = 0.0021). G3PDH assay showed a marked increase of intracellular enzyme activity in group 1 compared with in group 2 (0.61 +/- 0.10 versus 0.34 +/- 0.13 U/mL, P = 0.00045). Histology revealed normal structures of fragmental fatty tissues in both groups. While adipose aspirates by both modalities maintain normal structure, the LipiVage system yields a greater number of viable adipocytes and sustains a higher level of intracellular enzyme activity within fat grafts and can potentially be a preferred method of choice for large-quantity fat graft harvesting.

Polymer Therapy: A Novel Treatment to Improve Fat Graft Viability

“…4,31 Others have suggested that spin speeds above 50 g may be injurious to adipocytes. 32 Other authors have found no differences in centrifugation speeds at all. 33 When Kurita et al 31 examined adipocytes with scanning electron microscopy, they noted membrane pore injury at all spin speeds.…”

Autologous Fat Grafting

“…Use of smaller syringes or low-vacuum devices has been corroborated by in vitro experiments showing greater adipocyte viability and metabolic function compared to conventional liposuction or larger syringe aspiration. 34,35 In a prospective cohort study, Pu et al 36 found that the Coleman technique produced higher numbers of viable adipocytes than conventional liposuction. Conversely, Smith et al 37 found no significant difference in adipocyte viability between syringe aspiration and liposuction methods; although the bolus injection method used in that study may be responsible for the low graft take and results may change with a more robust sample size.…”

Ist eine intramuskuläre Injektion bei autologer Fetttransplantation zur Mamma sinnvoll ? - Eine MRT-volumetrische Studie

“…[17] , verschiedener Lokalan ä sthetika [14] , unterschiedlicher Absaugmethoden [7] ebenso untersucht wie der Einfl uss einer Zentrifugation zur Aufbereitung des Fetttransplantates [4] . Hierbei wurden Vitalit ä tstests angewendet, die Aussagen ü ber die Qualit ä t des Fetttransplantates geben k ö nnen.…”