The vascular and glandular organoprotective properties of metronidazole in the rodent stomach

Abstract

INTRODUCTIONHelicobacter pylori is known to play a signi®cant aetiopathogenetic role in peptic ulcer disease. Triple therapy using colloidal bismuth subcitrate in combination with amoxycillin and metronidazole has been shown not only to heal peptic ulcers but also to prevent their recurrence. 1, 2 In a previous study, we demonstrated that amoxycillin, in addition to its antimicrobial action, imparted gastric cytoprotection through the action of prostaglandins in the gastric mucosa. 3 The present study aimed to determine whether metronidazole, one of the most commonly used antimicrobials for anti-Helicobacter pylori therapy, has similar organoprotective properties. In order to test this hypothesis, both ethanol and indomethacin ulcer models were employed in the rats. Evaluation of gastric mucosal mucus, prostaglandin E 2 (PGE 2 ) content, blood¯ow and potential difference across the mucosa were performed because these parameters have been shown to be the key factors in the maintenance of gastric mucosal integrity. 4±8 Furthermore, the effects of metronidazole on the vascular and glandular integrity of the stomachs of SUMMARY Background: The gastroprotective action of metronidazole, an antimicrobial used in the therapy against Helicobacter pylori infection, is unclear. Thus, the aim of the present investigation was to study the organoprotective action and antiulcer mechanisms of this drug in rodents. Methods and results: Metronidazole (10 mg/kg), given either per os or intraperitoneally, 30 min beforehand, reduced ethanol (40%, 10 mL/kg, p.o.)-induced gastric mucosal damage in male rats. Likewise, oral administration of metronidazole dose-dependently attenuated the indomethacin (30 mg/kg, p.o.)-induced gastric lesion formation and the concurrent depletion of mucosal mucus. However, metronidazole did not affect the basal mucosal prostaglandin E 2 content. In an ex vivo gastric chamber preparation, 40% ethanol incubation markedly lowered transmucosal potential

Unravelling the In Vitro and In Vivo Anti-Helicobacter pylori Effect of Delphinidin-3-O-Glucoside Rich Extract from Pomegranate Exocarp: Enhancing Autophagy and Downregulating TNF-α and COX2

“…Treat.) group (infected rats were given metronidazole at a dose of 10 mg/kg orally and daily after 7 days of the last H. pylori inoculation and the treatment continued for one week) [ 23 ]; and pomegranate and metronidazole co-treated (Pom + Met. Treat.)…”

Metronidazole may display anti‐inflammatory features in periodontitis treatment: A scoping review

“…In the 14 animal studies, 11 experiments were performed on rats (Acikan et al, 2022;Arndt et al, 1994;Cardoso et al, 2011;Davies & Jamali, 1997;Fararjeh et al, 2008;Ganrotnorlin et al, 1981;Garrido-Mesa et al, 2011;Ko et al, 1997;Lehmann et al, 2006;Leite et al, 2001;Naumov et al, 2015) and 5 of them on different animal models such as mice (Becker et al, 2016;Fararjeh et al, 2008;Nishimuta & Ito, 2003;Seidler Stangova et al, 2019) and rabbits (Ko et al, 1997).…”

“…Seven of the experimental designs used oral gavage as the method of administration (Arndt et al, 1994;Becker et al, 2016;Davies & Jamali, 1997;Ganrotnorlin et al, 1981;Garrido-Mesa et al, 2011;Leite et al, 2001;Naumov et al, 2015), two intraperitoneal injections (Fararjeh et al, 2008;Ko et al, 1997), one orogastric tube (Ko et al, 1997), one IV short infusion (Lehmann et al, 2006), one drinking water (Seidler Stangova et al, 2019), one oinment (Nishimuta & Ito, 2003), one MTZ paste (Cardoso et al, 2011), and one gel (Acikan et al, 2022). The systemic conditions used for the experiments were heterogeneous and included induced arthritis (Ganrotnorlin et al, 1981), induced intestinal inflammation (Arndt et al, 1994), induced damage to the gastric mucosa (Ko et al, 1997) Arndt et al (1994) To evaluate the anti-inflammatory action of MTZ inhibiting leukocyte-endothelial cell adhesion in postcapillary venules Rats MTZ (by oral gavage) at 100 mg/kg 24 h and 12 h before the experiment MTZ may exert an anti-inflammatory effect by altering the ability of leukocytes to adhere to microvascular endothelium and/or subsequently migrate into the extravascular compartment. Ko et al (1997) To evaluate the anti-inflammatory effect of MTZ in peptic ulcer treatment…”

“…The systemic conditions used for the experiments were heterogeneous and included induced arthritis (Ganrotnorlin et al, 1981), induced intestinal inflammation (Arndt et al, 1994), induced damage to the gastric mucosa (Ko et al, 1997) Arndt et al (1994) To evaluate the anti-inflammatory action of MTZ inhibiting leukocyte-endothelial cell adhesion in postcapillary venules Rats MTZ (by oral gavage) at 100 mg/kg 24 h and 12 h before the experiment MTZ may exert an anti-inflammatory effect by altering the ability of leukocytes to adhere to microvascular endothelium and/or subsequently migrate into the extravascular compartment. Ko et al (1997) To evaluate the anti-inflammatory effect of MTZ in peptic ulcer treatment…”

“…If MTZ plays a role in controlling inflammation, it is possible that this agent might also influence tissue repair. Multiple in vitro (Rizzo et al, 2010;Siawasch et al, 2022), in vivo (animal) (Acikan et al, 2022;Cardoso et al, 2011;Ganrotnorlin et al, 1981;Ko et al, 1997;Naumov et al, 2015), and human studies (Buyuk & Kavala, 2000;Tanga et al, 1975) have explored the activity of MTZ as a modulator of inflammation in scarring and reparative processes. Cardoso et al, evaluated the effect of an MTZ paste on the healing of dry sockets with or without infection in rats.…”