The Variation of Soil Phosphorus Fractions and Microbial Community Composition under Consecutive Cucumber Cropping in a Greenhouse

Abstract

The distribution of phosphorus (P) fractions in soil plays a decisive role in soil P bioavailability; however, the characteristics of soil P fractions under consecutive cropping in a solar greenhouse remain unclear. To evaluate the effects of the long-term successive vegetable cropping on soil P fractions and the microbial community composition in greenhouse soil, a continuous long-term cropping experiment was conducted using cucumber (Cucumis sativus L.) in a solar greenhouse starting from 2006 to 2018. Soil P fractions and the microbial community composition were determined using the Hedley continuous extraction method and the phospholipid fatty acid (PLFA) method, respectively, in the 1st, 9th, 13th, and 21st rounds of cultivation. The soil total phosphorus (TP) content increased from 0.90 g·kg−1 in the 1st round to 3.07 g·kg−1 in the 21st round of cucumber cultivation. With an increase in continuous cropping rounds, soil available phosphorus (AP) increased and the phosphorus activation coefficient (PAC) decreased, with no significant difference between the 13th and 21st rounds. After 21 rounds of continuous cropping, the soil organic matter (SOM) content was 16.34 g·kg−1, 1.42 times that of the 1st round. The abundance of soil bacteria, actinomycetes, Gram-negative bacteria (G−), Gram-positive bacteria (G+), and total PLFAs initially increased with continuous cropping rounds, but then decreased significantly, and the ratios of fungi:bacteria (F/B) and G+/G− bacteria also increased significantly with continuous cropping rounds. The contents of soil labile P, moderately labile P, and non-labile P increased significantly over 21 continuous cropping rounds. Together, these results demonstrate that long-term continuous cropping can directly lead to the accumulation of P fractions, but it can also affect the abundance of actinomycetes through SOM enrichment, which indirectly leads to the accumulation of non-labile P. This study provides a theoretical basis for future soil P fertilizer management and vegetable production sustainability.

Unveiling the ecological significance of phosphorus fractions in shaping bacterial and archaeal beta diversity in mesotrophic lakes

“…More recently, such relationship between P fractions and microbes is widely concerned in terrestrial ecosystems ( Yang et al, 2023 ), but it is still largely missing in aquatic ecosystems. For example, microbial population have a crucial role in forming or releasing soil labile phosphorus across Tibetan alpine grasslands ( Li et al, 2022 ), while P fractions can also influence the abundance of Actinomycetes by governing the enrichment of soil organic matter ( Bian et al, 2022 ). Especially in rhizosphere soils, calcium-bound P can effectively inhibit the increase of microbial composition and biomass P ( Teng et al, 2018 ).…”