The value of MMP-9 for breast and non-small cell lung cancer patients' survival

Schveigert, Diana; Cicėnas, Saulius; Bruz̆as, S.; Samalavicius, NE; Gudlevičienė, Živilė; Didžiapetrienė, Janina

doi:10.2478/v10039-012-0066-y

Cited by 27 publications

(19 citation statements)

References 24 publications

(23 reference statements)

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“…CAFs promote the growth of cancer cells by providing nutrition to tumor cells (23,24), and facilitate the invasion and metastasis of tumor cells by secreting matrix metalloprotease to resolve the matrix surrounding cancer cells (16,25–28). Additionally, CAFs activate tumor angiogenesis (29–31).…”

Section: Differential Expressions Of Certain Special Genes In Cafsmentioning

confidence: 99%

Genetic alterations and epigenetic alterations of cancer-associated fibroblasts

Che

2016

Oncology Letters

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Cancer-associated fibroblasts (CAFs) are one major type of component identified in the tumor microenvironment. Studies have focused on the genetic and epigenetic status of CAFs, since they are critical in tumor progression and differ phenotypically and functionally from normal fibroblasts. The present review summarizes the recent achievements in understanding the gene profiles of CAFs and pays special attention to their possible epigenetic alterations. A total of 7 possible genetic alterations and epigenetic changes in CAFs are discussed, including gene differential expression, karyotype analysis, gene copy number variation, loss of heterozygosis, allelic imbalance, microsatellite instability, post-transcriptional control and DNA methylation. These genetic and epigenetic characteristics are hypothesized to provide a deep understanding of CAFs and a perspective on their clinical significance.

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Section: Differential Expressions Of Certain Special Genes In Cafsmentioning

confidence: 99%

Genetic alterations and epigenetic alterations of cancer-associated fibroblasts

Che

2016

Oncology Letters

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“…The expression levels of MMP-9 have been reported in gastric carcinoma, bladder, breast, and lung cancers [13][14][15]. To better understand the relationship between RhoGDI2 and MMP-9 in lung cancer, the expression levels and activities of MMP-9 were tested in the A549, control shRNA, and RhoGDI2 shRNA cell lines.…”

Section: The Down-regulation Of Rhogdi2 Showed the Increased Invasivementioning

confidence: 99%

RNA interference-mediated knockdown of RhoGDI2 induces the migration and invasion of human lung cancer A549 cells via activating the PI3K/Akt pathway

Huang

Yang

et al. 2014

Tumor Biol.

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Rho GDP dissociation inhibitor 2 (RhoGDI2) has been identified as a tumor suppressor gene for cellular migration and invasion. However, the underlying mechanism and effector targets of RhoGDI2 in lung cancer are still not fully understood. In this study, a vector-expressed small hairpin RNA (shRNA) of RhoGDI2 was transfected into the human lung cancer cell line A549. After the successful transfection, the down-regulation of RhoGDI2 promoted the proliferation, migration, and invasion of lung cancer cells in vitro through the increasing expression and activities of the matrix metallopeptidase 9 (MMP-9) and PI3K/Akt pathways. Transiently transfecting the small interfering RNA (siRNA) of MMP-9 into the RhoGDI2 shRNA cells reduced the MMP-9 expression. Both transfecting the siRNA and adding the MMP-9 antibody into the RhoGDI2 shRNA cells led to a decrease in the invasion and migration of the lung cancer cells. The blockade of the PI3K/Akt pathway by LY294002 resulted in abolishment of the effects of RhoGDI2 shRNA in Akt phosphorylation and MMP-9 expression. This result suggests that the down-regulated RhoGDI2 contributed to the migration and invasion of the lung cancer cell line via activating the PI3K/Akt pathway and the ensuing increase in the expression and activity of MMP-9. In conclusion, we report that the shRNA-mediated knockdown of RhoGDI2 induces the invasion and migration of lung cancer due to cross-talk with the PI3K/Akt pathway and MMP-9. Verifying the role and molecular mechanism of the participation of RhoGDI2 in the migration and invasion of lung cancer may provide a target for better treatment.

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“…A study found that the expression of MMP9 is upergulated in cancer tissues (Benson et al, 2013). Another study found that MMP9, MMP11 and MMP28 mRNA expression was higher in breast cancer tissue, also the survival rate was lower for the patients with positive MMP9 expression (Schveigert et al, 2013). While a study on human brain glioma found that gelatinase-B (MMP9) is primarily involved in tumor neovascularization (Forsyth et al, 1999).…”

Section: Discussion:-mentioning

confidence: 99%

“…The Matrix metalloproteinases MMPs comprise a family of 24 zinc dependent endopeptidases with broad spectrum of enzymatic activity against all components of the extracellular matrix ( ECM ) and basement membrane (Schveigert et al, 2013). Based on their structure and substrate specificity.…”

Section: …………………………………………………………………………………………………… Introduction:-mentioning

confidence: 99%