The value of immunofluorescence techniques in the diagnosis of bullous disorders: a review

Black, Martin M.; Thomas, Rhian; Bhogal, B.

doi:10.1111/j.1365-2230.1983.tb01792.x

Cited by 10 publications

(6 citation statements)

References 86 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The technique of immunofluorescence (IF) is widely used in the diagnosis of primary bullous diseases of the skin (PBD) and the methods and characteristic findings in various conditions have been reviewed recently (Black, Meyrick Thomas & Bhogal, 1983;Allen & Reeves, 1983)-We have used direct IF of skin biopsies and examination of sera by indirect IF throughout the last 9 years. Our initial experience in a series of no patients has been reported (Reeves et al, 1978) and we now present data obtained in a total of 279 consecutive patients, in order to review the value of IF in the investigation and management of PBD.…”

mentioning

confidence: 99%

Skin immunofluorescence in the diagnosis of primary bullous diseases-a review of 279 cases

et al. 1986

View full text Add to dashboard Cite

Summary Immunofluorescence (IF) findings are reviewed in 279 consecutive patients with a suspected primary bullous disorder (PBD), IF substantiated the diagnosis of PBD in 51%. The most frequent disorders were bullous pemphigoid (44%), dermatitis herpetiformis (25%) and pemphigus (13%) A diagnosis of PBD was refuted in 29% of cases with bullae; whereas 18% of cases presenting with an itchy papular eruption were shown to have a PBD. All 19 patients with intercellular epidermal IgG ± C3 on direct IF had a clinical and histological diagnosis of pemphigus. Thirty‐five patients with granular sub‐epidermal IgA ± C3 had dermatitis herpetiformis. Of the 72 with linear C3 ± IgG at the dermo‐epidermal junction (DEJ), 62 had bullous pemphigoid, seven herpes gestationis, two cicatricial pemphigoid, and one epidermolysis bullosa acquisita. The 13 patients with linear IgA as the predominant immunoglobulin at the DEJ appeared to form a distinct clinical group, i.e. linear IgA disease. Deposition of IgA at the DEJ appeared to be a marker for mucosal disease in the series as a whole. The presence of a second immunoglobulin at the DEJ (in addition to IgG) in patients with bullous pemphigoid was associated with more severe disease. In 50%, of the biopsies IF was either negative or showed non‐specific patterns of staining which did not associate with any particular clinical feature: of these, the most common were diffuse dermal IgG, dermal fibrinogen, granular C3 at the DEJ and vascular deposits. IF examination is of considerable value in the diagnosis and management of PBD provided that non‐specific patterns of deposition are interpreted appropriately.

show abstract

mentioning

confidence: 99%

Skin immunofluorescence in the diagnosis of primary bullous diseases-a review of 279 cases

et al. 1986

View full text Add to dashboard Cite

show abstract

“…[5] 1. Direct immunofluorescence (DIF): DIF is a one step procedure that involves application of fluoresceinated antibodies to a frozen section of the skin.…”

Section: Immunodermatological Methodsmentioning

confidence: 99%

Immunofluorescence in dermatology

Chhabra¹,

Minz²,

Saikia³

2012

Indian J Dermatol Venereol Leprol

View full text Add to dashboard Cite

Direct immunofluorescence (DIF) and indirect immunofluorescence (IIF) tests on skin biopsy are being done mostly in academic teaching hospitals. These tests provide a useful diagnostic aid to dermatologists. Immunohistology and serology can, in conjunction with histology, provide considerable help in delineation and diagnosis of various skin disorders as well as systemic diseases with skin involvement, e.g. systemic lupus erythematosus. Immunofluorescence (IF) studies have now become an invaluable supplement to clinical and histological examination in a variety of dermatological diseases. These skin diseases now include not only bullous and connective tissue disorders, vasculitides, and conditions such as lichen planus, but also the scaling dermatoses, notably psoriasis. In this review article, we share our experience of providing such a diagnostic facility for more than 30 years in a large tertiary care health center in North India and also help to outline the conditions, which can be diagnosed confidently, and others where IF can help in confirming a diagnosis or the immune component of the disease. The article also deals with handling of skin biopsy specimens and interpretation of biopsy findings on DIF and IIF examination.

show abstract

“…Although DIF is the gold standard for the diagnosis of pemphigus the exact pemphigus subtype can be determined by histology of early vesicles. 7,9,10 A previous study by Diya et al found positive predictive value of 100% by DIF in the pemphigus group of disorders and negative predictive value of 85-90%. 1 In our study a positive predictive value of 100% and negative predictive value of 100% was found.…”

Section: Paraneoplastic Pemphigusmentioning

confidence: 94%

Clinicopathological and immunofluorescence study of vesiculobullous disorders

2019

View full text Add to dashboard Cite

INTRODUCTIONPatients with vesiculobullous diseases form a significant percentage of both outpatient and inpatient cases attending the Department of Dermatology and Venereology. The diagnois of vesiculobullous diseases is based on clinical correlation and histopathological findings. Majority of vesiculobullous disease sareimmune mediated, hence immunofluorescence as a diagnostic aid in dermatology has revolutionized the diagnostic capability as well as understanding of the pathogenic mechanisms of vesiculobullous diseases. Although both direct and indirect immunofluorescence can be used, it is the former which helps in identifying the pattern and exact site of immunoglobulin deposition. 1 AimThe aim was to identify the immunofluorescence pattern in vesiculobullous diseases and study the correlation between clinical histopathological and immunofluorescence patterns. ABSTRACTBackground: Vesiculobullous diseases are mostly immune mediated and diagnosed based on the clinical features, histology and Immunofluorescence. The aim of the study was to identify the immunofluorescence pattern in auto immune vesiculobullous diseases and correlate it with the clinical profile and histology. Methods: Patients attending the dermatology outpatient department in a tertiary hospital with vesiculobullous diseases, suggestive of auto immune aetiology were evaluated clinically. Histopathology and direct immunofluorescence were done in all patients. Results: During the one year period from June 2008 to July 2009, 40 patients with vesiculobullous disorders clinically suggestive of auto immune aetiology attended the outpatient department. Out of the 40 patients, 22 (55%) patients were diagnosed to have intraepidermal with female preponderance and 18 patients (45%) sub epidermal blistering diseases. Bullous pemphigoid was the commonest sub epidermal disease, seen in 8 patients. Conclusions:In all cases diagnosed clinically as pemphigus a histological diagnosis of pemphigus was made (100%). The clinical variants of pemphigus could also be diagnosed in all cases histologically (100%). The positive and negative predictive value was 100% in pemphigus group cases. Histology of all patients showed subepidermal bulla (100%). A specific diagnosis could be made in 18 patients with sub epidermal disease (100%). DIF was found to be an invaluable tool in diagnosing different diseases belonging to the sub epidermal group, but it was not of much help in sub classifying variants of pemphigus.

show abstract

The value of immunofluorescence techniques in the diagnosis of bullous disorders: a review

Cited by 10 publications

References 86 publications

Skin immunofluorescence in the diagnosis of primary bullous diseases-a review of 279 cases

Skin immunofluorescence in the diagnosis of primary bullous diseases-a review of 279 cases

Immunofluorescence in dermatology

Clinicopathological and immunofluorescence study of vesiculobullous disorders

Contact Info

Product

Resources

About