The value of fast blastocoele re-expansion in the selection of a viable thawed blastocyst for transfer

Shu, Yaqing; Watt, J.; Gebhardt, J.; Dasig, J.; Appling, Julie; Behr, Barry

doi:10.1016/j.fertnstert.2007.11.083

Cited by 54 publications

(53 citation statements)

References 28 publications

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“…The fact that post-warm blastocysts with faster developmental kinetics resulted in higher implantation outcomes has been reported in humans [21,26,27] and sheep [28]. The criterion of fast-developing embryos employed in the present study (≥50% increase in diameter at 2-h culture) has been adopted from Shu et al [21].…”

Section: Discussionmentioning

confidence: 99%

“…Their cryosurvival was assessed by the presence of the blastocoelic cavity 2 h after the in vitro culture. Simultaneously, the survivors were morphologically graded according to Gardner's criteria [20] and were classified into two categories based on their developmental kinetics [21]. Surviving blastocysts with≥50% increase in their embryonic diameter were defined as fast-developing embryos.…”

Section: Culture and Transfer Of Post-warm Blastocystsmentioning

confidence: 99%

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In vitro and in vivo viability of human blastocysts collapsed by laser pulse or osmotic shock prior to vitrification

Iwayama¹,

Hochi

Yamashita³

2010

J Assist Reprod Genet

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Purpose This study was designed to investigate whether artificial shrinkage, induced by a laser pulse or hyperosmotic sucrose solutions, improves in vitro survival and/or implantation of vitrified-warmed human expanded blastocysts. Methods Before Cryotop vitrification, the blastocoelic cavity was collapsed either by a laser pulse or sucrose solutions. Non-treated blastocysts were used as control. Post-warm blastocyst survival and implantation after transfer were examined. Implantation rate outcome was retrospectively analyzed by morphological grading and developmental kinetics of post-warm blastocysts. Results Survival rates in the three groups were high. Implantation rates in the laser-pulse group (59.7%) were comparable with those in the sucrose group (49.3%), and were significantly higher than those in the control group (34.2%). The proportion of blastocysts showing fast development tended to be higher when the blastocysts underwent artificial shrinkage treatment before vitrification. There was no clear correlation between morphology of post-warm blastocysts and implantation rate. Conclusion Artificial shrinkage treatment before vitrification is associated with an increased probability of fast-developing embryos, resulting in higher implantation rates.

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Section: Discussionmentioning

confidence: 99%

Section: Culture and Transfer Of Post-warm Blastocystsmentioning

confidence: 99%

In vitro and in vivo viability of human blastocysts collapsed by laser pulse or osmotic shock prior to vitrification

Iwayama¹,

Hochi

Yamashita³

2010

J Assist Reprod Genet

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“…The re-expansion grade after warming has been used to indicate blastocyst survivability and quality after vitrification [21][22][23][24][25][26] and the clinical pregnancy rate of rapidly re-expanding blastocysts (within 2-4 h) was more than double the rate of slowly re-expanding blastocysts [21]. In this study, blastocyst degeneration was not observed after TE biopsy and only euploid blastocysts were selected for embryo transfer.…”

Section: Discussionmentioning

confidence: 92%

The optimal timing of blastocyst vitrification after trophectoderm biopsy of preimplantation genetic screening

Lee

2016

Fertility and Sterility

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“…The remaining good-quality blastocysts on day 5 and day 6 were frozen and thawed using the Menezo two-step slow freezing and two-step rapid thawing protocols as previously described [3]. No pregnancy was achieved from her fresh embryo transfer.…”

Section: Casementioning

confidence: 99%

Transfer of IVF-contaminated blastocysts with removal of the zona pellucida resulted in live births

Shu

Prokai

Berga

et al. 2016

J Assist Reprod Genet

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Purpose Bacterial contamination may cause loss or damage to cultured oocytes or embryos, resulting in cancelation or delaying of a fresh embryo transfer. While live births have been reported following the transfer of embryos contaminated with yeast, very little information is available on how to handle embryos with bacterial contamination. We report two cases of successful pregnancy in patients with bacterial contamination of embryo culture dishes. Methods We retrospectively reviewed 878 oocyte retrievals performed between January 2011 and December 2014. Bacterial contamination was recorded in two split IVF/ICSI cases, where contamination occurred in embryo culture drops containing embryos from conventional insemination but not from ICSI on day 3. Results To minimize the adverse effects of bacterial contamination on transfer outcomes, we removed the zona pellucida of contaminated frozen blastocysts and successfully obtained clinical pregnancies following transfer of zona-free blastocysts that were previously contaminated during IVF culture. Conclusions Removal of the zona pellucida is an appropriate approach to handle blastocysts contaminated with bacteria during in vitro culture.

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The value of fast blastocoele re-expansion in the selection of a viable thawed blastocyst for transfer

Cited by 54 publications

References 28 publications

In vitro and in vivo viability of human blastocysts collapsed by laser pulse or osmotic shock prior to vitrification

In vitro and in vivo viability of human blastocysts collapsed by laser pulse or osmotic shock prior to vitrification

The optimal timing of blastocyst vitrification after trophectoderm biopsy of preimplantation genetic screening

Transfer of IVF-contaminated blastocysts with removal of the zona pellucida resulted in live births

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