The value of combined use of survivin, cytokeratin 20 and mucin 7 mRNA for bladder cancer detection in voided urine

Abstract

Combined use of the three markers can improve the sensitivity for detecting bladder cancer.

Effect of pathogenic bacteria on reliability of CK-19, CK-20 and UPII as bladder cancer genetic markers: A molecular biology study

“…Similarly, El-Salahy [15] reported a significant correlation between CK-20 mRNA expression and tumor stage. However, there was no correlation with the tumor grade in the present study, a finding which is not coping with the results of ElSalahy [15] but is coping with Pu, Wang [8]. Uroplakins (UPs) are integral membrane proteins that are synthesized as the major differentiation products of mammalian urothelium.…”

“…However, cystoscopy is invasive and may be ineffective in patients with an indwelling catheter or active inflammation due to the presence of abnormal appearance of the bladder mucosa, whereas cytology has low sensitivity in low-grade papillary disease. Additional lab-based markers are needed to aid in the evaluation of these lesions [8]. Genetic marker development represents a promising direction for the diagnosis of bladder cancer [27].…”

“…However, up to 25% of bladder cancer patients may not have hematuria, even when they have a known bladder tumor [8,9]. Urine cytology is a microscopic examination for urine samples to search for any cancer or precancer cells.…”

“…Another cytokeratin is "CK-20", which is expressed in urothelium of the bladder cancer patients. It can be considered as a marker of urothelial differentiation [8]. However, despite the fact that malignant cells generally retain the intermediate filaments of the progenitor, normal cells do not express the CK-20 gene.…”

“…Screening for bladder cancer, in patients with previous symptoms to look for different substances or cancer cells in the urine is currently done with urinalysis, urinary cytology and cystoscopy [8].…”