Effect of pathogenic bacteria on reliability of CK-19, CK-20 and UPII as bladder cancer genetic markers: A molecular biology study

Abstract

a b s t r a c t Objectives: To evaluate the effect of the presence of pathogenic bacteria in urine on three bladder cancer genetic markers (Cytokeratin 19, Cytokeratin 20 and Uroplakin II mRNA), and to evaluate the reliability of each urine marker separately. Methods: Voided urine samples from 20 bladder cancer patients, 15 patients with urinary tract infection patients and 10 healthy volunteers were collected. Isolation and identification of bacteria was performed followed by determination of antimicrobial susceptibility of isolates. Evaluation of CK-19, CK-20 and UPII mRNA in urine by RT-PCR was carried out.Results: The most frequent organism isolated was Escherichia coli followed by Klebsiella pneumoniae. The overall sensitivity and specificity were 47.37% and 68.42%% for CK-19, 57.89% and 100% for CK-20 and 63.1% and 100%for UPII. Combined sensitivity and specificity of CK-20 and UPII biomarkers together was higher than that of each biomarker alone or even more than that of the three combined biomarkers. Conclusions: E. coli is the most predominant bacteria isolated from bladder cancer patients.Both CK-20 and UPII have different expression levels for both benign and malignant cases.Combined use of UPII and CK-20 may be a promising noninvasive tool for the detection of bladder cancer in urine for patients who have both symptoms of UTI and cancer.

Escherichia coli foster bladder cancer cell line progression via epithelial mesenchymal transition, stemness and metabolic reprogramming

“…Reduction of epithelial markers have been highlighted in our previous research among infected bladder cancer patients less than those without infection 18 . This finding goes hand in hand with study performed by Leone et al 20 as it was reported K. pneumoniae ability to promote EMT-like programs in airway epithelial cells through direct interaction.…”

“…This study reveals that bacterial infection triggers EMT features such as reduction of epithelial marker (CK19) and upregulation of mesenchymal marker (vimentin). Reduction of epithelial markers have been highlighted in our previous research among infected bladder cancer patients less than those without infection 18 . This finding goes hand in hand with study performed by Leone et al 20 as it was reported K .…”

“…Escherichia coli strain was isolated from urine sample of bladder cancer patient during our previous work 18 . Urine sample collection was done according to ethical committee approval from Mansoura University (RP/41).…”

Evaluation of cytokeratin 19 as a prognostic tumoral and metastatic marker with focus on improved detection methods

“…CK19 is one of these tumor markers as a result of its expression in the uroepithelial cells. CYFRA 21‐1 has been applied as a prognostic and diagnostic tumor marker for middle cell neoplasm of the urothelium (El Shobaky, Abbas, Raouf, Zakaria, & Ali‐El‐Dein, ).…”

The effects of biofilms on tumor progression in a 3D cancer-biofilm microfluidic model

“…The device was designed to fit a 100-mm dish, filled with a thin film of sterile deionized water and stored in a humidified chamber to minimize the evaporation of long-term cultures (Berthier et al 2008). Since UTI caused by UPEC infection is a contributing factor to the development of bladder cancer, and UPEC is similarly associated with bladder cancer (Chagneau et al 2020;El Shobaky et al 2015), we utilized bladder cancer cells (UMUC-3) and the UPEC strain (UTI89) in this proof-of-concept study. The cells were uniformly suspended in the growth medium and seeded into each microchannel.…”